Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103253
• Name: oriT_pMCR16_P053
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain YL14P053
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_KY352406 (21206..21305 [+], 100 nt)
• oriT length: 100 nt
• IRs (inverted repeats): 25..32, 35..42 (TAACCTGT..ACAGGTTA)
• Location of nic site: 61..62
• Conserved sequence flanking the nic site: TCCTGCCCGG
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 100 nt

>oriT_pMCR16_P053
GAATAAGGGGAGAGTAAAGTTTGATAACCTGTTTACAGGTTAGCTAACTTTACATGTCCTGCCCGGCTTTCGCCGGTGTTTACGCCAAGGAAAGTTTACA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_BTN48_RS00115_pMCR16_P053

• ID: 2431
• GenBank: WP_077789841
• Name: Relaxase_BTN48_RS00115_pMCR16_P053
• UniProt ID: _
• Length: 870 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 870 a.a.
• Molecular weight: 96964.93 Da
• Isoelectric Point: 10.5259

>WP_077789841.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MRNVKKSSFVELAKYLQDPQNKQERVGVIKVTNCHQENILDAARYDIEPTQRRNTRSEGDKTYHLVISFR
PGEKPSQEVLDAAEERICAAMGYADHQRVSAVHYDTDNVHIHIAINKIHPQTHNIHTPYNDYKTRSAVCL
ALEKEFGLESDNHRASKTRSENNAADMERHSGRESLLGWIKRECADQLKSAANWQELHQAMRQNGLELRQ
KGNGFVITGPDGLAVKASSVDRSLSRAALEKKLGSFESSVPDTKPSRPSPEAIKQRFGSAAHVKRPGDRP
PPMRENRQVNLGDVPVMQINSGKRYEGKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAKVKRDRLI
ENAKRSGRLKRATIKLLKGPGVNKRYLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWADWLQFKA
AGGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGKRLDVS
RDAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKKAAREREY
IHIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDAGRERSGN
GNATGANEPGVTGRSTSADAGNGYGTSSSRRRTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPNGERGHSK
PNVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGAGGITTPA
EVKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIVVMPVSDY
TARRLSNLKVGESVTLTPSGSVRTTKGRSR

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (5-244)

• Domain of unknown function (DUF5710) (DUF5710) (558-599)

• Large polyvalent protein-associated domain 7 (LPD7) (464-548)

Protein structure

No available structure.

T4CP

t4cp2_BTN48_RS00105_pMCR16_P053

• ID: 2197
• GenBank: WP_032310210
• Name: t4cp2_BTN48_RS00105_pMCR16_P053
• UniProt ID: _
• Length: 648 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 648 a.a.
• Molecular weight: 71711.82 Da
• Isoelectric Point: 8.3842

>WP_032310210.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAVAANDDASNEAIAI

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (119-418)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (446-629)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 35167..45485

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTN48_RS00200	31100..31567	-	468	WP_048207657	antirestriction protein	-
BTN48_RS00310	31657..31926	-	270	WP_228719037	hypothetical protein	-
BTN48_RS00315	32028..32240	-	213	WP_223307920	hypothetical protein	-
BTN48_RS00210	32237..32389	-	153	WP_172689268	hypothetical protein	-
BTN48_RS00215	33310..34188	-	879	WP_048207660	plasmid replication initiator TrfA	-
BTN48_RS00330	34326..34472	-	147	WP_048207679	single-stranded DNA-binding protein	-
BTN48_RS00225	34578..34982	+	405	WP_048207662	transcriptional regulator	-
BTN48_RS00230	35167..36123	+	957	WP_112933759	P-type conjugative transfer ATPase TrbB	virB11
BTN48_RS00235	36237..36620	+	384	WP_003019650	TrbC/VirB2 family protein	virB2
BTN48_RS00240	36623..36949	+	327	WP_003019648	conjugal transfer protein TrbD	virB3
BTN48_RS00245	36946..39489	+	2544	WP_112933760	VirB4 family type IV secretion/conjugal transfer ATPase	virb4
BTN48_RS00250	39486..40193	+	708	WP_112933762	VirB8/TrbF family protein	virB8
BTN48_RS00255	40209..41099	+	891	WP_003019641	P-type conjugative transfer protein TrbG	virB9
BTN48_RS00260	41102..41554	+	453	WP_032194780	conjugal transfer protein TrbH	-
BTN48_RS00265	41558..42952	+	1395	WP_048207667	TrbI/VirB10 family protein	virB10
BTN48_RS00270	42965..43735	+	771	WP_046960647	P-type conjugative transfer protein TrbJ	virB5
BTN48_RS00275	43748..43921	+	174	WP_024252956	entry exclusion lipoprotein TrbK	-
BTN48_RS00280	43926..45485	+	1560	WP_197747621	P-type conjugative transfer protein TrbL	virB6
BTN48_RS00285	45496..45801	+	306	WP_032310194	lipoprotein	-
BTN48_RS00290	45817..46587	+	771	WP_048207671	conjugal transfer protein	-
BTN48_RS00320	46604..47071	+	468	WP_250645731	TraX family protein	-
BTN48_RS00325	47026..47289	+	264	WP_250645732	TraX family protein	-

Host bacterium

• ID: 3696
• GenBank: NZ_KY352406
• Plasmid name: pMCR16_P053
• Incompatibility group: IncP1
• Plasmid size: 47824 bp
• Coordinate of oriT [Strand]: 21206..21305 [+]
• Host baterium: Salmonella enterica subsp. enterica serovar Typhimurium strain YL14P053

Cargo genes

• Drug resistance gene: mcr-1.6
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -