Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103251 |
| Name | oriT_pR150626  |
| Organism | Salmonella enterica subsp. enterica serovar Typhimurium strain R150626 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KY120366 (47490..47542 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pR150626
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2195 | GenBank | WP_015059539 |
| Name | t4cp2_HTK57_RS00155_pR150626 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 6254..29711
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTK57_RS00015 (AAIOCJHD_00001) | 2112..2618 | + | 507 | WP_001326595 | CaiF/GrlA family transcriptional regulator | - |
| HTK57_RS00020 (AAIOCJHD_00002) | 2611..2826 | + | 216 | WP_001127357 | DUF1187 family protein | - |
| HTK57_RS00025 (AAIOCJHD_00003) | 2819..2995 | + | 177 | WP_000753050 | hypothetical protein | - |
| HTK57_RS00030 (AAIOCJHD_00004) | 3022..3975 | + | 954 | WP_072089442 | SPFH domain-containing protein | - |
| HTK57_RS00035 (AAIOCJHD_00005) | 4349..4792 | + | 444 | WP_000964330 | NfeD family protein | - |
| HTK57_RS00040 (AAIOCJHD_00006) | 4796..4966 | + | 171 | WP_000550720 | hypothetical protein | - |
| HTK57_RS00045 (AAIOCJHD_00007) | 4977..5423 | + | 447 | WP_001243165 | hypothetical protein | - |
| HTK57_RS00050 (AAIOCJHD_00009) | 5643..5996 | + | 354 | WP_223286767 | hypothetical protein | - |
| HTK57_RS00055 (AAIOCJHD_00010) | 5993..6250 | + | 258 | WP_000739144 | hypothetical protein | - |
| HTK57_RS00060 (AAIOCJHD_00011) | 6254..7249 | + | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| HTK57_RS00065 (AAIOCJHD_00012) | 7255..7896 | + | 642 | WP_001425343 | type IV secretion system protein | - |
| HTK57_RS00070 (AAIOCJHD_00013) | 7898..8152 | + | 255 | WP_001043555 | EexN family lipoprotein | - |
| HTK57_RS00075 (AAIOCJHD_00014) | 8165..8452 | + | 288 | WP_001032611 | EexN family lipoprotein | - |
| HTK57_RS00080 (AAIOCJHD_00015) | 8525..9160 | + | 636 | WP_015059536 | hypothetical protein | - |
| HTK57_RS00085 (AAIOCJHD_00016) | 9260..9511 | + | 252 | WP_000121741 | hypothetical protein | - |
| HTK57_RS00090 (AAIOCJHD_00017) | 9501..9782 | + | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTK57_RS00095 (AAIOCJHD_00018) | 9850..10149 | + | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTK57_RS00100 (AAIOCJHD_00019) | 10152..11387 | + | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| HTK57_RS00105 (AAIOCJHD_00020) | 11393..11830 | + | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HTK57_RS00110 (AAIOCJHD_00021) | 11949..12347 | + | 399 | WP_001153665 | hypothetical protein | - |
| HTK57_RS00115 (AAIOCJHD_00022) | 12368..12952 | + | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| HTK57_RS00380 (AAIOCJHD_00023) | 12952..13242 | + | 291 | WP_000865479 | conjugal transfer protein | - |
| HTK57_RS00125 (AAIOCJHD_00024) | 13313..13633 | + | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTK57_RS00130 (AAIOCJHD_00025) | 13639..15996 | + | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| HTK57_RS00135 (AAIOCJHD_00027) | 16162..16896 | + | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTK57_RS00140 (AAIOCJHD_00028) | 16962..17663 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTK57_RS00145 (AAIOCJHD_00029) | 17653..18792 | + | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| HTK57_RS00150 (AAIOCJHD_00030) | 18811..19866 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTK57_RS00155 (AAIOCJHD_00031) | 19882..21840 | + | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HTK57_RS00160 (AAIOCJHD_00032) | 21887..22423 | + | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HTK57_RS00165 (AAIOCJHD_00033) | 22416..24059 | + | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| HTK57_RS00170 (AAIOCJHD_00034) | 24098..25420 | + | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTK57_RS00175 (AAIOCJHD_00035) | 25404..25898 | + | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTK57_RS00180 (AAIOCJHD_00036) | 25923..27461 | + | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTK57_RS00185 (AAIOCJHD_00037) | 27452..28561 | + | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTK57_RS00190 (AAIOCJHD_00038) | 28606..29163 | + | 558 | WP_000095048 | type 4 pilus major pilin | - |
| HTK57_RS00195 (AAIOCJHD_00039) | 29229..29711 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTK57_RS00200 (AAIOCJHD_00040) | 29715..30350 | + | 636 | WP_000934977 | A24 family peptidase | - |
| HTK57_RS00205 (AAIOCJHD_00041) | 30363..31679 | + | 1317 | WP_021500066 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTK57_RS00210 (AAIOCJHD_00042) | 31658..31966 | - | 309 | WP_001199277 | hypothetical protein | - |
| HTK57_RS00215 (AAIOCJHD_00043) | 32015..33139 | + | 1125 | WP_000486716 | site-specific integrase | - |
| HTK57_RS00220 (AAIOCJHD_00044) | 33151..33804 | + | 654 | WP_170855322 | hypothetical protein | - |
| HTK57_RS00225 (AAIOCJHD_00045) | 33834..34366 | + | 533 | Protein_44 | thermonuclease family protein | - |
Host bacterium
| ID | 3694 | GenBank | NZ_KY120366 |
| Plasmid name | pR150626 | Incompatibility group | IncI2 |
| Plasmid size | 59651 bp | Coordinate of oriT [Strand] | 47490..47542 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Typhimurium strain R150626 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |