Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103245 |
| Name | oriT_pP111  |
| Organism | Salmonella enterica subsp. enterica serovar Typhimurium strain P111 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KY120365 (48204..48256 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pP111
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2191 | GenBank | WP_015059539 |
| Name | t4cp2_HTK64_RS00135_pP111 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 6246..29076
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTK64_RS00010 (LMCCDMEO_00001) | 2104..2610 | + | 507 | WP_001326595 | CaiF/GrlA family transcriptional regulator | - |
| HTK64_RS00015 (LMCCDMEO_00002) | 2603..2818 | + | 216 | WP_001127357 | DUF1187 family protein | - |
| HTK64_RS00020 (LMCCDMEO_00003) | 2811..2987 | + | 177 | WP_000753050 | hypothetical protein | - |
| HTK64_RS00025 (LMCCDMEO_00004) | 3014..3967 | + | 954 | WP_072089442 | SPFH domain-containing protein | - |
| HTK64_RS00030 (LMCCDMEO_00005) | 4341..4784 | + | 444 | WP_000964330 | NfeD family protein | - |
| HTK64_RS00035 (LMCCDMEO_00006) | 4788..4958 | + | 171 | WP_000550720 | hypothetical protein | - |
| HTK64_RS00040 (LMCCDMEO_00007) | 4969..5415 | + | 447 | WP_001243165 | hypothetical protein | - |
| HTK64_RS00045 (LMCCDMEO_00009) | 5635..5988 | + | 354 | WP_223286767 | hypothetical protein | - |
| HTK64_RS00050 (LMCCDMEO_00010) | 5985..6242 | + | 258 | WP_000739144 | hypothetical protein | - |
| HTK64_RS00055 (LMCCDMEO_00011) | 6246..7241 | + | 996 | WP_001028540 | type IV secretion system protein | virB6 |
| HTK64_RS00060 (LMCCDMEO_00012) | 7247..7891 | + | 645 | WP_001310442 | type IV secretion system protein | - |
| HTK64_RS00065 (LMCCDMEO_00013) | 7900..8124 | + | 225 | WP_000713562 | EexN family lipoprotein | - |
| HTK64_RS00070 (LMCCDMEO_00014) | 8347..9156 | - | 810 | WP_024237698 | DUF5710 domain-containing protein | - |
| HTK64_RS00075 (LMCCDMEO_00015) | 9204..9503 | + | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTK64_RS00080 (LMCCDMEO_00016) | 9506..10741 | + | 1236 | WP_034169417 | toxin co-regulated pilus biosynthesis Q family protein | - |
| HTK64_RS00085 (LMCCDMEO_00017) | 10747..11184 | + | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| HTK64_RS00090 (LMCCDMEO_00018) | 11303..11701 | + | 399 | WP_001153669 | hypothetical protein | - |
| HTK64_RS00095 (LMCCDMEO_00019) | 11722..12306 | + | 585 | WP_001177117 | lytic transglycosylase domain-containing protein | virB1 |
| HTK64_RS00375 (LMCCDMEO_00020) | 12306..12596 | + | 291 | WP_000865479 | conjugal transfer protein | - |
| HTK64_RS00105 (LMCCDMEO_00021) | 12667..12987 | + | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTK64_RS00110 (LMCCDMEO_00022) | 12993..15350 | + | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| HTK64_RS00115 (LMCCDMEO_00024) | 15516..16250 | + | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTK64_RS00120 (LMCCDMEO_00025) | 16316..17017 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTK64_RS00125 (LMCCDMEO_00026) | 17007..18146 | + | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| HTK64_RS00130 (LMCCDMEO_00027) | 18165..19220 | + | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTK64_RS00135 (LMCCDMEO_00028) | 19236..21194 | + | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HTK64_RS00140 (LMCCDMEO_00029) | 21241..21777 | + | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HTK64_RS00145 (LMCCDMEO_00030) | 21770..23413 | + | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| HTK64_RS00150 (LMCCDMEO_00031) | 23452..24774 | + | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTK64_RS00155 (LMCCDMEO_00032) | 24758..25252 | + | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTK64_RS00160 (LMCCDMEO_00033) | 25277..26815 | + | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTK64_RS00165 (LMCCDMEO_00034) | 26806..27915 | + | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTK64_RS00170 (LMCCDMEO_00035) | 27965..28528 | + | 564 | WP_034169414 | type 4 pilus major pilin | - |
| HTK64_RS00175 (LMCCDMEO_00036) | 28594..29076 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTK64_RS00180 (LMCCDMEO_00037) | 29080..29715 | + | 636 | WP_000934977 | A24 family peptidase | - |
| HTK64_RS00185 (LMCCDMEO_00038) | 29728..31002 | + | 1275 | WP_235883108 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTK64_RS00400 | 31021..31239 | + | 219 | Protein_37 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTK64_RS00195 | 31610..31894 | + | 285 | WP_235883107 | pilus assembly protein | - |
| HTK64_RS00200 | 32252..32407 | + | 156 | WP_001358489 | hypothetical protein | - |
| HTK64_RS00205 (LMCCDMEO_00041) | 32730..33854 | + | 1125 | WP_000486716 | site-specific integrase | - |
Host bacterium
| ID | 3688 | GenBank | NZ_KY120365 |
| Plasmid name | pP111 | Incompatibility group | IncI2 |
| Plasmid size | 60960 bp | Coordinate of oriT [Strand] | 48204..48256 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Typhimurium strain P111 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |