Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103241
• Name: oriT_GW-Imi-1b1|unnamed3
• Organism: Citrobacter braakii strain GW-Imi-1b1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP115730 (11748..11884 [-], 137 nt)
• oriT length: 137 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 137 nt

>oriT_GW-Imi-1b1|unnamed3
CGAATGGTCTGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGCCATCCTGTTAAGCTCATCTGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACGTGCTTTGCAACAAGCATAAAAGGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_PGH06_RS00080_GW-Imi-1b1|unnamed3

• ID: 2423
• GenBank: WP_272487428
• Name: Relaxase_PGH06_RS00080_GW-Imi-1b1|unnamed3
• UniProt ID: _
• Length: 889 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 889 a.a.
• Molecular weight: 101339.83 Da
• Isoelectric Point: 9.8030

>WP_272487428.1 TraI/MobA(P) family conjugative relaxase [Citrobacter braakii]
MIVKKVPNSKTKHKTGSIRDLADYIREPHNQNPDEKVLYANGRGFLCDGHAAQREEMVALAAESVRSKNP
VNHYIMSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNG
FDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAIE
DGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQ
QVAQREPEPIKPDVPGWKDYITGRKAHYAAKNAAKLALDKQQEQERKQLAEQQKARRDELMRGNWNGKGE
VLNAMRSVVAAEQAAEKAALKEKHKKQREQWRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGA
SGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGEGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSF
TVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRVT
SIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKKHHILIDDMNREKLE
RLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRK
PKHQREDGSYPEVRLLKVERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAY
QRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARYA
YSAAGDRQAAELGKYRQQWEKVEGREPVRQQEQAKAQKIERDNSPGMSR

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (17-272)

• Large polyvalent protein-associated domain 7 (LPD7) (438-526)

• RepB DNA-primase from phage plasmid (RepB_primase) (548-806)

Protein structure

No available structure.

Host bacterium

• ID: 3684
• GenBank: NZ_CP115730
• Plasmid name: GW-Imi-1b1|unnamed3
• Incompatibility group: IncP6
• Plasmid size: 28609 bp
• Coordinate of oriT [Strand]: 11748..11884 [-]
• Host baterium: Citrobacter braakii strain GW-Imi-1b1

Cargo genes

• Drug resistance gene: catB2, blaOXA-2, blaOXA-10, qacE, sul1
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -