Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103232
Name   oriT_GW-Imi-1b1|unnamed5 in_silico
Organism   Citrobacter braakii strain GW-Imi-1b1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP115732 (10002..10138 [-], 137 nt)
oriT length   137 nt
IRs (inverted repeats)      28..33, 37..42  (ACATGC..GCATGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT_GW-Imi-1b1|unnamed5
CGAATGGGCAGGCGGGGATTGGCAGGAACATGCGTAGCATGTGTATGACAATCCGCATCCTGTATTGCTCAACCACTCTACTATCATATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCATAAAAGGCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2416 GenBank   WP_047353284
Name   Relaxase_PGH06_RS00065_GW-Imi-1b1|unnamed5 insolico UniProt ID   A0A2H5CQB3
Length   890 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 890 a.a.        Molecular weight: 101914.42 Da        Isoelectric Point: 9.7193

>WP_047353284.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Pseudomonadota]
MIVKKVPNPKKSASKAQRIGQLTSYVRSPESESPQEKCLYAGARGFMMDDPKSQTAEMIALSQEAVRSKD
TINHYVLSWREGEQPSPEQVEEAVSIFMDELGWKDHQAIYGLHSDTDNIHLHIVINRVHPETLKIVEKNR
GFDIELAHKAIARIEHAQGWQREQNGRYQVLENGELGRAPYDPEKPRQPDQKKRDMENRTGEKSAHRIAI
EDGAAIIKQAQTWEQLHRELAAKGMRYEKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQR
QQVAPREPEPIKPDVPGWKDYITGRKAHYAEKNADKLAQDKRQEQERKQLAEQQKARRDELMRGNWKGKG
EVLNAMRSVIAAEQAAEKAALKEKHQKEREQHRQRFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEG
DRSEPPTPRDIRAYQPEIVGQQVHYSRKEEAGRGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGS
FTVMGNDEYKAMCGKLAAEHGFKITNPELQESIQQERQRIQQERVQAMKSEQLKQFERYAEAVGAERYRV
TSIKMREDGSKQTFILDKKDGITRGFTPQEIEQRTPEMQRLQRRGENLYYTPLSDKKHHILIDDMNREKL
ERLIRDGYQPAAVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR
KPKHQREDGSYPEVRLLKAERRECIKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDA
YQRHYRDVLKRQRGGEVDLSRLDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARY
AYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSL

  Protein domains


Predicted by InterproScan.

RepB DNA-primase from phage plasmid (RepB_primase)

(548-807)

Relaxase/Mobilisation nuclease domain (Relaxase)

(45-274)

Large polyvalent protein-associated domain 7 (LPD7)

(439-527)


  Protein structure


Source ID Structure
AlphaFold DB A0A2H5CQB3


Host bacterium

ID   3675 GenBank   NZ_CP115732
Plasmid name   GW-Imi-1b1|unnamed5 Incompatibility group   IncQ2
Plasmid size   14211 bp Coordinate of oriT [Strand]   10002..10138 [-]
Host baterium   Citrobacter braakii strain GW-Imi-1b1

Cargo genes

Drug resistance gene   aph(3')-Ia
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -