Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103227
Name   oriT_pSE_AH228 in_silico
Organism   Salmonella sp. strain AH228
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_ON960346 (214881..215135 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pSE_AH228
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2413 GenBank   WP_012477394
Name   TraI_2_PRZ79_RS01185_pSE_AH228 insolico UniProt ID   A0A2Z2E7B6
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118067.47 Da        Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Z2E7B6


T4CP

ID   2179 GenBank   WP_001284073
Name   traD_PRZ79_RS01190_pSE_AH228 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 116945..126152

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PRZ79_RS00635 (CPHELBEB_03572) 112383..112595 - 213 WP_001285422 Hha/YmoA family nucleoid-associated regulatory protein -
PRZ79_RS00640 (CPHELBEB_03573) 112766..113839 + 1074 WP_272487357 tyrosine-type recombinase/integrase -
PRZ79_RS00645 113886..114590 + 705 WP_001067858 IS6-like element IS26 family transposase -
PRZ79_RS00650 (CPHELBEB_03575) 114643..115392 + 750 Protein_129 RepB family plasmid replication initiator protein -
PRZ79_RS00655 (CPHELBEB_03577) 116541..116894 + 354 WP_000423602 hypothetical protein -
PRZ79_RS00660 (CPHELBEB_03578) 116912..117262 + 351 WP_001447770 type IV conjugative transfer system protein TraL -
PRZ79_RS00665 (CPHELBEB_03579) 117274..118062 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
PRZ79_RS00670 (CPHELBEB_03580) 118062..119333 + 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
PRZ79_RS00675 (CPHELBEB_03581) 119335..119796 + 462 WP_000521242 plasmid transfer protein HtdO -
PRZ79_RS00680 (CPHELBEB_03582) 119786..121141 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
PRZ79_RS00685 (CPHELBEB_03583) 121149..121631 + 483 WP_000377632 hypothetical protein -
PRZ79_RS00690 (CPHELBEB_03584) 121750..122502 + 753 WP_182911723 protein-disulfide isomerase HtdT -
PRZ79_RS00695 (CPHELBEB_03585) 122512..123462 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
PRZ79_RS00700 (CPHELBEB_03586) 123471..126152 + 2682 WP_000387412 TraC family protein virb4
PRZ79_RS00705 127249..127404 + 156 WP_001371891 hypothetical protein -
PRZ79_RS00710 128701..128862 + 162 WP_001371949 hypothetical protein -

Host bacterium

ID   3670 GenBank   NZ_ON960346
Plasmid name   pSE_AH228 Incompatibility group   -
Plasmid size   283086 bp Coordinate of oriT [Strand]   214881..215135 [-]
Host baterium   Salmonella sp. strain AH228

Cargo genes

Drug resistance gene   blaTEM-1B, aph(3')-Ia, aph(6)-Id, sul1, qacE, ere(A), aac(6')-IIc, catA2, tet(D)
Virulence gene   spvC, spvB
Metal resistance gene   pcoE, pcoC, pcoB, pcoA, silP, silA, silB, silF, silC, silR, silS, silE, terE, terD, terC, terB, terA, terZ, terW
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -