Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103223
Name   oriT_C6661|unnamed in_silico
Organism   Klebsiella pneumoniae strain C6661
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_ON111447 (68940..69040 [-], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_C6661|unnamed
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2411 GenBank   WP_012561166
Name   TrwC_PRZ73_RS00515_C6661|unnamed insolico UniProt ID   D7RU03
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB D7RU03


Auxiliary protein

ID   1028 GenBank   WP_001749975
Name   WP_001749975_C6661|unnamed insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6

ID   1029 GenBank   WP_001254386
Name   WP_001254386_C6661|unnamed insolico UniProt ID   A0A3Z6KJB5
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9005.11 Da        Isoelectric Point: 10.1422

>WP_001254386.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Gammaproteobacteria]
MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVTEESES
TFKEL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-61)


  Protein structure


Source ID Structure
AlphaFold DB A0A3Z6KJB5

ID   1030 GenBank   WP_000124981
Name   WP_000124981_C6661|unnamed insolico UniProt ID   A0A630FTW9
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14542.59 Da        Isoelectric Point: 5.0278

>WP_000124981.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]
MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLELGLRVYDAQMERKESAFNQTEFNKLL
LECVVKTQSTVAKILGIESLSPHVSGNPKFEYASMVDDIREKVSVEMDRFFPKNDDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A630FTW9


T4CP

ID   2177 GenBank   WP_021740568
Name   t4cp2_PRZ73_RS00510_C6661|unnamed insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57738.89 Da        Isoelectric Point: 9.6550

>WP_021740568.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacteriaceae]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVILWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 91079..101404

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PRZ73_RS00610 86978..87229 - 252 Protein_122 IS30 family transposase -
PRZ73_RS00615 (CCDJINMC_00120) 87262..88653 - 1392 WP_014839878 ISKra4-like element ISKpn19 family transposase -
PRZ73_RS00620 (CCDJINMC_00121) 88690..89262 - 573 WP_001493762 recombinase family protein -
PRZ73_RS00625 (CCDJINMC_00122) 89399..89944 - 546 WP_001493763 plasmid pRiA4b ORF-3 family protein -
PRZ73_RS01285 (CCDJINMC_00123) 90085..90372 + 288 Protein_126 DUF6710 family protein -
PRZ73_RS00635 (CCDJINMC_00124) 90546..91079 - 534 WP_000792636 phospholipase D family protein -
PRZ73_RS00640 (CCDJINMC_00125) 91079..92074 - 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
PRZ73_RS00645 (CCDJINMC_00126) 92116..93276 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
PRZ73_RS00650 (CCDJINMC_00127) 93276..94160 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
PRZ73_RS00655 (CCDJINMC_00128) 94171..94869 - 699 WP_000646594 type IV secretion system protein virB8
PRZ73_RS01290 94859..95017 - 159 WP_012561180 hypothetical protein -
PRZ73_RS00660 (CCDJINMC_00129) 95088..96128 - 1041 WP_001749958 type IV secretion system protein virB6
PRZ73_RS00665 (CCDJINMC_00130) 96144..96371 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
PRZ73_RS00670 (CCDJINMC_00131) 96379..97092 - 714 WP_001749960 type IV secretion system protein virB5
PRZ73_RS00675 (CCDJINMC_00132) 97110..99710 - 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
PRZ73_RS00680 (CCDJINMC_00133) 99710..100027 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
PRZ73_RS00685 (CCDJINMC_00134) 100077..100370 - 294 WP_001749962 hypothetical protein virB2
PRZ73_RS00690 (CCDJINMC_00135) 100380..100661 - 282 WP_000440698 transcriptional repressor KorA -
PRZ73_RS00695 (CCDJINMC_00136) 100670..101404 - 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
PRZ73_RS00700 (CCDJINMC_00137) 101468..101818 + 351 WP_024129965 H-NS family nucleoid-associated regulatory protein -
PRZ73_RS00705 (CCDJINMC_00138) 101834..102178 + 345 WP_001749964 hypothetical protein -
PRZ73_RS00710 (CCDJINMC_00139) 102175..102489 + 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
PRZ73_RS00715 (CCDJINMC_00140) 102603..102836 + 234 WP_001191790 hypothetical protein -
PRZ73_RS00720 (CCDJINMC_00141) 102886..103533 + 648 WP_015344958 restriction endonuclease -
PRZ73_RS00725 (CCDJINMC_00142) 103538..103744 + 207 WP_001749967 hypothetical protein -
PRZ73_RS00730 103755..104027 - 273 Protein_147 IS1 family transposase -
PRZ73_RS00735 (CCDJINMC_00143) 104126..105559 + 1434 WP_001288432 DNA cytosine methyltransferase -

Host bacterium

ID   3666 GenBank   NZ_ON111447
Plasmid name   C6661|unnamed Incompatibility group   IncFII
Plasmid size   183190 bp Coordinate of oriT [Strand]   68940..69040 [-]
Host baterium   Klebsiella pneumoniae strain C6661

Cargo genes

Drug resistance gene   fosA3, blaCTX-M-65, blaKPC-2, blaSHV-12, qnrS1, blaNDM-1, dfrA14, rmtB
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9