Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103221
Name   oriT1_C6770|unnamed in_silico
Organism   Klebsiella pneumoniae strain C6770
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_ON111448 (49548..49597 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  TGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT1_C6770|unnamed
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2409 GenBank   WP_012561166
Name   TrwC_PRZ70_RS00475_C6770|unnamed insolico UniProt ID   D7RU03
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB D7RU03


Auxiliary protein

ID   1024 GenBank   WP_001749975
Name   WP_001749975_C6770|unnamed insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6

ID   1025 GenBank   WP_001254386
Name   WP_001254386_C6770|unnamed insolico UniProt ID   A0A3Z6KJB5
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9005.11 Da        Isoelectric Point: 10.1422

>WP_001254386.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Gammaproteobacteria]
MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVTEESES
TFKEL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-61)


  Protein structure


Source ID Structure
AlphaFold DB A0A3Z6KJB5

ID   1026 GenBank   WP_000124981
Name   WP_000124981_C6770|unnamed insolico UniProt ID   A0A630FTW9
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14542.59 Da        Isoelectric Point: 5.0278

>WP_000124981.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]
MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLELGLRVYDAQMERKESAFNQTEFNKLL
LECVVKTQSTVAKILGIESLSPHVSGNPKFEYASMVDDIREKVSVEMDRFFPKNDDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A630FTW9


T4CP

ID   2175 GenBank   WP_021740568
Name   t4cp2_PRZ70_RS00480_C6770|unnamed insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57738.89 Da        Isoelectric Point: 9.6550

>WP_021740568.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacteriaceae]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVILWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 18037..28362

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PRZ70_RS00110 13936..14187 - 252 Protein_22 IS30 family transposase -
PRZ70_RS00115 (KAKMAGNC_00020) 14220..15611 - 1392 WP_014839878 ISKra4-like element ISKpn19 family transposase -
PRZ70_RS00120 (KAKMAGNC_00021) 15648..16220 - 573 WP_001493762 recombinase family protein -
PRZ70_RS00125 (KAKMAGNC_00022) 16357..16902 - 546 WP_001493763 plasmid pRiA4b ORF-3 family protein -
PRZ70_RS01295 17043..17330 + 288 Protein_26 DUF6710 family protein -
PRZ70_RS00135 (KAKMAGNC_00023) 17504..18037 - 534 WP_000792636 phospholipase D family protein -
PRZ70_RS00140 (KAKMAGNC_00024) 18037..19032 - 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
PRZ70_RS00145 (KAKMAGNC_00025) 19074..20234 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
PRZ70_RS00150 (KAKMAGNC_00026) 20234..21118 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
PRZ70_RS00155 (KAKMAGNC_00027) 21129..21827 - 699 WP_000646594 type IV secretion system protein virB8
PRZ70_RS01300 21817..21975 - 159 WP_012561180 hypothetical protein -
PRZ70_RS00160 (KAKMAGNC_00028) 22046..23086 - 1041 WP_001749958 type IV secretion system protein virB6
PRZ70_RS00165 (KAKMAGNC_00029) 23102..23329 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
PRZ70_RS00170 (KAKMAGNC_00030) 23337..24101 - 765 WP_013149461 type IV secretion system protein virB5
PRZ70_RS00175 (KAKMAGNC_00031) 24068..26668 - 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
PRZ70_RS00180 (KAKMAGNC_00032) 26668..26985 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
PRZ70_RS00185 (KAKMAGNC_00033) 27035..27328 - 294 WP_001749962 hypothetical protein virB2
PRZ70_RS00190 (KAKMAGNC_00034) 27338..27619 - 282 WP_000440698 transcriptional repressor KorA -
PRZ70_RS00195 (KAKMAGNC_00035) 27628..28362 - 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
PRZ70_RS00200 (KAKMAGNC_00036) 28426..28776 + 351 WP_024129965 H-NS family nucleoid-associated regulatory protein -
PRZ70_RS00205 (KAKMAGNC_00037) 28792..29136 + 345 WP_001749964 hypothetical protein -
PRZ70_RS00210 (KAKMAGNC_00038) 29133..29447 + 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
PRZ70_RS00215 (KAKMAGNC_00039) 29561..29794 + 234 WP_001191790 hypothetical protein -
PRZ70_RS00220 (KAKMAGNC_00040) 29844..30491 + 648 WP_015344958 restriction endonuclease -
PRZ70_RS00225 (KAKMAGNC_00041) 30496..30702 + 207 WP_001749967 hypothetical protein -
PRZ70_RS00230 30713..30985 - 273 Protein_47 IS1 family transposase -
PRZ70_RS00235 (KAKMAGNC_00042) 31084..32517 + 1434 WP_001288432 DNA cytosine methyltransferase -

Host bacterium

ID   3664 GenBank   NZ_ON111448
Plasmid name   C6770|unnamed Incompatibility group   IncN
Plasmid size   184378 bp Coordinate of oriT [Strand]   49548..49597 [+]; 67702..67802 [+]
Host baterium   Klebsiella pneumoniae strain C6770

Cargo genes

Drug resistance gene   fosA3, blaNDM-1, dfrA14, blaCTX-M-65, qnrS1, blaSHV-12, blaKPC-2, rmtB
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9