Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103220
Name   oriT_pMbl536 in_silico
Organism   Escherichia coli strain Mbl536
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KY689635 (21736..21808 [+], 73 nt)
oriT length   73 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 73 nt

>oriT_pMbl536
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2408 GenBank   WP_000991384
Name   Relaxase_HTN93_RS00120_pMbl536 insolico UniProt ID   A0A8H8Z9E5
Length   903 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 903 a.a.        Molecular weight: 104595.99 Da        Isoelectric Point: 7.6377

>WP_000991384.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGY
TTVQCESLIEDMIAGGNFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNADRCVILCEPGGTPLYEDTGVLEARLQKDGS
VRFRDRRNGELVCVDYGDRVVFYHHQDRNELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAW
HNAAGITGSGHFVISRPDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure



No available structure.




Auxiliary protein

ID   1023 GenBank   WP_001291058
Name   WP_001291058_pMbl536 insolico UniProt ID   A0A7I0L241
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12730.52 Da        Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L241


T4CP

ID   2174 GenBank   WP_172692493
Name   trbC_HTN93_RS00085_pMbl536 insolico UniProt ID   _
Length   767 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 767 a.a.        Molecular weight: 86935.97 Da        Isoelectric Point: 7.3008

>WP_172692493.1 F-type conjugative transfer protein TrbC [Escherichia coli]
MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIWPLALPVCLLFTLVTSVIFMLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHII
FFSTTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEV
IQSGDKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSMLLARDLGYRLE
GTDAQTLEVKKYKGSFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLTASPPHKKNVAQTPSGGKQESRKKRF

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 65873..98954

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTN93_RS00385 61104..62723 + 1620 WP_021532696 PilN family type IVB pilus formation outer membrane protein -
HTN93_RS00390 62744..64039 + 1296 WP_077250483 type 4b pilus protein PilO2 -
HTN93_RS00395 64029..64481 + 453 WP_021532698 type IV pilus biogenesis protein PilP -
HTN93_RS00400 64530..65084 + 555 WP_021532699 hypothetical protein -
HTN93_RS00405 65180..65776 + 597 WP_029391917 hypothetical protein -
HTN93_RS00410 65873..67381 + 1509 WP_106631238 ATPase, T2SS/T4P/T4SS family virB11
HTN93_RS00415 67383..68483 + 1101 WP_086795366 type II secretion system F family protein -
HTN93_RS00420 68533..69069 + 537 WP_172692504 type 4 pilus major pilin -
HTN93_RS00425 69128..69604 + 477 WP_053287759 lytic transglycosylase domain-containing protein virB1
HTN93_RS00430 69564..70271 + 708 WP_215421957 A24 family peptidase -
HTN93_RS00435 70268..71476 + 1209 WP_172692505 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTN93_RS00440 72528..73697 + 1170 WP_134233607 site-specific integrase -
HTN93_RS00445 73726..74019 + 294 WP_236918885 hypothetical protein -
HTN93_RS00450 74138..74683 + 546 WP_072674828 hypothetical protein -
HTN93_RS00455 74934..75756 + 823 Protein_89 conjugal transfer protein TraE -
HTN93_RS00460 75846..77048 + 1203 WP_077896580 conjugal transfer protein TraF -
HTN93_RS00465 77407..77859 + 453 WP_001081199 DotD/TraH family lipoprotein -
HTN93_RS00470 77856..78692 + 837 WP_000848138 type IV secretory system conjugative DNA transfer family protein traI
HTN93_RS00475 78676..79824 + 1149 WP_097313008 plasmid transfer ATPase TraJ virB11
HTN93_RS00480 79821..80111 + 291 WP_000817799 hypothetical protein traK
HTN93_RS00485 80175..84236 + 4062 WP_097313009 LPD7 domain-containing protein -
HTN93_RS00490 84253..84603 + 351 WP_021532713 hypothetical protein traL
HTN93_RS00495 84615..85310 + 696 WP_029391932 DotI/IcmL family type IV secretion protein traM
HTN93_RS00500 85321..86295 + 975 WP_021532715 DotH/IcmK family type IV secretion protein traN
HTN93_RS00505 86299..87630 + 1332 WP_097313011 conjugal transfer protein TraO traO
HTN93_RS00510 87627..88340 + 714 WP_097313012 conjugal transfer protein TraP traP
HTN93_RS00515 88337..88867 + 531 WP_227316726 conjugal transfer protein TraQ traQ
HTN93_RS00520 88914..89312 + 399 WP_021532718 DUF6750 family protein traR
HTN93_RS00530 89535..90353 + 819 WP_157921211 conjugal transfer protein TraT traT
HTN93_RS00535 90363..90767 + 405 WP_236918857 hypothetical protein -
HTN93_RS00540 90767..91147 + 381 WP_065898666 hypothetical protein -
HTN93_RS00545 91323..94367 + 3045 WP_172692506 ATP-binding protein traU
HTN93_RS00550 94367..94984 + 618 WP_029391943 hypothetical protein traV
HTN93_RS00555 94945..96150 + 1206 WP_029391944 conjugal transfer protein TraW traW
HTN93_RS00560 96147..96716 + 570 WP_058100845 conjugal transfer protein TraX -
HTN93_RS00565 96789..98954 + 2166 WP_029391945 DotA/TraY family protein traY
HTN93_RS00570 99042..99695 + 654 WP_087526029 plasmid IncI1-type surface exclusion protein ExcA -

Host bacterium

ID   3663 GenBank   NZ_KY689635
Plasmid name   pMbl536 Incompatibility group   IncB/O/K/Z
Plasmid size   100230 bp Coordinate of oriT [Strand]   21736..21808 [+]
Host baterium   Escherichia coli strain Mbl536

Cargo genes

Drug resistance gene   mcr-1.1, sul2, blaTEM-1B
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -