Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103215
Name   oriT_pMbl488 in_silico
Organism   Escherichia coli strain Mbl488
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KY565558 (21275..21347 [+], 73 nt)
oriT length   73 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 73 nt

>oriT_pMbl488
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2406 GenBank   WP_000991384
Name   Relaxase_HTP15_RS00115_pMbl488 insolico UniProt ID   A0A8H8Z9E5
Length   903 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 903 a.a.        Molecular weight: 104595.99 Da        Isoelectric Point: 7.6377

>WP_000991384.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGY
TTVQCESLIEDMIAGGNFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNADRCVILCEPGGTPLYEDTGVLEARLQKDGS
VRFRDRRNGELVCVDYGDRVVFYHHQDRNELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAW
HNAAGITGSGHFVISRPDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure



No available structure.




Auxiliary protein

ID   1020 GenBank   WP_001291058
Name   WP_001291058_pMbl488 insolico UniProt ID   A0A7I0L241
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12730.52 Da        Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L241


T4CP

ID   2170 GenBank   WP_172692493
Name   trbC_HTP15_RS00080_pMbl488 insolico UniProt ID   _
Length   767 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 767 a.a.        Molecular weight: 86935.97 Da        Isoelectric Point: 7.3008

>WP_172692493.1 F-type conjugative transfer protein TrbC [Escherichia coli]
MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIWPLALPVCLLFTLVTSVIFMLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHII
FFSTTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEV
IQSGDKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSMLLARDLGYRLE
GTDAQTLEVKKYKGSFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLTASPPHKKNVAQTPSGGKQESRKKRF

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 66709..99789

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTP15_RS00390 61940..63559 + 1620 WP_172692728 PilN family type IVB pilus formation outer membrane protein -
HTP15_RS00395 63580..64875 + 1296 WP_172692729 type 4b pilus protein PilO2 -
HTP15_RS00400 64865..65317 + 453 WP_021532698 type IV pilus biogenesis protein PilP -
HTP15_RS00405 65366..65920 + 555 WP_021532699 hypothetical protein -
HTP15_RS00410 66016..66612 + 597 WP_029391917 hypothetical protein -
HTP15_RS00415 66709..68217 + 1509 WP_106631238 ATPase, T2SS/T4P/T4SS family virB11
HTP15_RS00420 68219..69319 + 1101 WP_086795366 type II secretion system F family protein -
HTP15_RS00425 69369..69905 + 537 WP_172692504 type 4 pilus major pilin -
HTP15_RS00430 69964..70440 + 477 WP_053287759 lytic transglycosylase domain-containing protein virB1
HTP15_RS00435 70400..71107 + 708 WP_236918856 A24 family peptidase -
HTP15_RS00440 71104..72363 + 1260 WP_172692730 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTP15_RS00445 73364..74533 + 1170 WP_134233607 site-specific integrase -
HTP15_RS00450 74574..74855 + 282 WP_029391927 hypothetical protein -
HTP15_RS00455 74974..75519 + 546 WP_072674828 hypothetical protein -
HTP15_RS00460 75770..76591 + 822 WP_000804089 hypothetical protein traE
HTP15_RS00465 76681..77883 + 1203 WP_172692731 conjugal transfer protein TraF -
HTP15_RS00590 77873..78004 - 132 WP_257792844 hypothetical protein -
HTP15_RS00470 78242..78694 + 453 WP_001081199 DotD/TraH family lipoprotein -
HTP15_RS00475 78691..79527 + 837 WP_000848138 type IV secretory system conjugative DNA transfer family protein traI
HTP15_RS00480 79511..80659 + 1149 WP_097313008 plasmid transfer ATPase TraJ virB11
HTP15_RS00485 80656..80946 + 291 WP_000817799 hypothetical protein traK
HTP15_RS00490 81010..85071 + 4062 WP_097313009 LPD7 domain-containing protein -
HTP15_RS00495 85088..85438 + 351 WP_021532713 hypothetical protein traL
HTP15_RS00500 85450..86145 + 696 WP_029391932 DotI/IcmL family type IV secretion protein traM
HTP15_RS00505 86156..87130 + 975 WP_021532715 DotH/IcmK family type IV secretion protein traN
HTP15_RS00510 87134..88465 + 1332 WP_097313011 conjugal transfer protein TraO traO
HTP15_RS00515 88462..89175 + 714 WP_097313012 conjugal transfer protein TraP traP
HTP15_RS00520 89172..89702 + 531 WP_227316726 conjugal transfer protein TraQ traQ
HTP15_RS00525 89749..90147 + 399 WP_021532718 DUF6750 family protein traR
HTP15_RS00595 90204..90455 + 252 WP_001277575 hypothetical protein -
HTP15_RS00535 90562..91188 + 627 WP_097487265 conjugal transfer protein TraT traT
HTP15_RS00540 91198..91602 + 405 WP_236918857 hypothetical protein -
HTP15_RS00545 91602..91982 + 381 WP_065898666 hypothetical protein -
HTP15_RS00550 92158..95202 + 3045 WP_172692506 ATP-binding protein traU
HTP15_RS00555 95202..95819 + 618 WP_029391943 hypothetical protein traV
HTP15_RS00560 95780..96985 + 1206 WP_029391944 conjugal transfer protein TraW traW
HTP15_RS00565 96982..97551 + 570 WP_058100845 conjugal transfer protein TraX -
HTP15_RS00570 97624..99789 + 2166 WP_029391945 DotA/TraY family protein traY
HTP15_RS00575 99877..100530 + 654 WP_087526029 plasmid IncI1-type surface exclusion protein ExcA -

Host bacterium

ID   3658 GenBank   NZ_KY565558
Plasmid name   pMbl488 Incompatibility group   IncB/O/K/Z
Plasmid size   101065 bp Coordinate of oriT [Strand]   21275..21347 [+]
Host baterium   Escherichia coli strain Mbl488

Cargo genes

Drug resistance gene   mcr-1.1, sul2, blaTEM-1B
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -