Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103211 |
| Name | oriT_pMcp0221  |
| Organism | Escherichia coli strain Mcp0221 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KY565557 (38279..38331 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pMcp0221
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2165 | GenBank | WP_063268641 |
| Name | t4cp2_HTL88_RS00055_pMcp0221 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73418.87 Da Isoelectric Point: 9.2394
>WP_063268641.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGYTAGYNLRFVFILQNEGQGQKSDMYGQEGWTTFTENSGVVLY
YPPKSKNSLAKKISEEIGVRDMKISKRSVSSGGGNTGTSRTRNDEIIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGYTAGYNLRFVFILQNEGQGQKSDMYGQEGWTTFTENSGVVLY
YPPKSKNSLAKKISEEIGVRDMKISKRSVSSGGGNTGTSRTRNDEIIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 751..17998
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTL88_RS00010 | 326..730 | + | 405 | WP_236918862 | hypothetical protein | - |
| HTL88_RS00015 | 751..1335 | + | 585 | WP_063268637 | lytic transglycosylase domain-containing protein | virB1 |
| HTL88_RS00020 | 1335..1625 | + | 291 | WP_063268638 | conjugal transfer protein | - |
| HTL88_RS00025 | 1696..2016 | + | 321 | WP_000362081 | VirB3 family type IV secretion system protein | virB3 |
| HTL88_RS00030 | 2022..4379 | + | 2358 | WP_063268639 | VirB4 family type IV secretion system protein | virb4 |
| HTL88_RS00035 | 4541..5275 | + | 735 | WP_015057166 | type IV secretion system protein | virB8 |
| HTL88_RS00040 | 5341..6042 | + | 702 | WP_049824867 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTL88_RS00045 | 6032..7171 | + | 1140 | WP_015057165 | TrbI/VirB10 family protein | virB10 |
| HTL88_RS00050 | 7190..8245 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTL88_RS00055 | 8261..10219 | + | 1959 | WP_063268641 | type IV secretory system conjugative DNA transfer family protein | - |
| HTL88_RS00060 | 10266..10709 | + | 444 | WP_089623547 | ATP-binding protein | virb4 |
| HTL88_RS00065 | 10702..12345 | + | 1644 | WP_045146416 | PilN family type IVB pilus formation outer membrane protein | - |
| HTL88_RS00070 | 12384..13706 | + | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTL88_RS00075 | 13690..14184 | + | 495 | WP_045146418 | type IV pilus biogenesis protein PilP | - |
| HTL88_RS00080 | 14209..15747 | + | 1539 | WP_045146419 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTL88_RS00085 | 15738..16847 | + | 1110 | WP_000974900 | type II secretion system F family protein | - |
| HTL88_RS00090 | 16892..17449 | + | 558 | WP_097728049 | type 4 pilus major pilin | - |
| HTL88_RS00095 | 17570..17998 | + | 429 | WP_001326801 | lytic transglycosylase domain-containing protein | virB1 |
| HTL88_RS00100 | 18002..18637 | + | 636 | WP_000934978 | A24 family peptidase | - |
| HTL88_RS00105 | 18650..19933 | + | 1284 | WP_172690226 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTL88_RS00110 | 20257..20517 | + | 261 | WP_198965019 | pilus assembly protein | - |
| HTL88_RS00450 | 20800..21021 | + | 222 | Protein_22 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTL88_RS00120 | 21434..22558 | + | 1125 | WP_172690227 | site-specific integrase | - |
Host bacterium
| ID | 3654 | GenBank | NZ_KY565557 |
| Plasmid name | pMcp0221 | Incompatibility group | IncI2 |
| Plasmid size | 64664 bp | Coordinate of oriT [Strand] | 38279..38331 [+] |
| Host baterium | Escherichia coli strain Mcp0221 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |