Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103205 |
| Name | oriT_pEC019  |
| Organism | Escherichia coli strain EC019 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KY471145 (15068..15120 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pEC019
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2157 | GenBank | WP_015059539 |
| Name | t4cp2_HTL15_RS00235_pEC019 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 33386..50728
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTL15_RS00170 (P019_00035) | 28804..29457 | - | 654 | WP_170855322 | hypothetical protein | - |
| HTL15_RS00175 (P019_00036) | 29469..30593 | - | 1125 | WP_000486716 | site-specific integrase | - |
| HTL15_RS00180 | 30915..31070 | - | 156 | WP_001358489 | hypothetical protein | - |
| HTL15_RS00185 (P019_00038) | 31494..32734 | - | 1241 | Protein_38 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTL15_RS00190 (P019_00039) | 32747..33382 | - | 636 | WP_000934977 | A24 family peptidase | - |
| HTL15_RS00195 (P019_00040) | 33386..33868 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTL15_RS00200 (P019_00041) | 33935..34492 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| HTL15_RS00205 (P019_00042) | 34537..35646 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTL15_RS00210 (P019_00043) | 35637..37175 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTL15_RS00215 (P019_00044) | 37200..37694 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTL15_RS00220 (P019_00045) | 37678..39000 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTL15_RS00225 (P019_00046) | 39039..40682 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| HTL15_RS00230 (P019_00047) | 40675..41211 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HTL15_RS00235 (P019_00048) | 41258..43216 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HTL15_RS00240 (P019_00049) | 43232..44287 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTL15_RS00245 (P019_00050) | 44306..45444 | - | 1139 | Protein_50 | TrbI/VirB10 family protein | - |
| HTL15_RS00250 (P019_00051) | 45434..46135 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTL15_RS00255 (P019_00052) | 46201..46935 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTL15_RS00260 (P019_00054) | 47101..49458 | - | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| HTL15_RS00265 (P019_00055) | 49464..49784 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTL15_RS00405 | 49855..50145 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HTL15_RS00275 (P019_00056) | 50030..50728 | - | 699 | WP_236918851 | lytic transglycosylase domain-containing protein | virB1 |
| HTL15_RS00280 (P019_00057) | 50749..51147 | - | 399 | WP_001153665 | hypothetical protein | - |
| HTL15_RS00285 (P019_00058) | 51266..51703 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HTL15_RS00290 (P019_00059) | 51709..52944 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| HTL15_RS00295 (P019_00060) | 52947..53246 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTL15_RS00300 (P019_00061) | 53314..53595 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTL15_RS00305 (P019_00062) | 53585..53836 | - | 252 | WP_000121741 | hypothetical protein | - |
| HTL15_RS00310 (P019_00063) | 53936..54571 | - | 636 | WP_015059536 | hypothetical protein | - |
| HTL15_RS00315 (P019_00064) | 54644..54931 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| HTL15_RS00320 (P019_00065) | 54944..55198 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
Host bacterium
| ID | 3648 | GenBank | NZ_KY471145 |
| Plasmid name | pEC019 | Incompatibility group | IncI2 |
| Plasmid size | 61572 bp | Coordinate of oriT [Strand] | 15068..15120 [-] |
| Host baterium | Escherichia coli strain EC019 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |