Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103195 |
| Name | oriT_pE15017_00  |
| Organism | Escherichia coli strain E15017_00 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KX772778 (18441..18493 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pE15017_00
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2147 | GenBank | WP_015059539 |
| Name | t4cp2_HTI99_RS00265_pE15017_00 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 36691..60148
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTI99_RS00200 (pE15017_040) | 32016..32669 | - | 654 | WP_170852113 | hypothetical protein | - |
| HTI99_RS00205 (pE15017_041) | 32681..33805 | - | 1125 | WP_000486716 | site-specific integrase | - |
| HTI99_RS00450 | 33868..34089 | + | 222 | Protein_44 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTI99_RS00215 | 34753..36039 | - | 1287 | WP_100783610 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTI99_RS00220 (pE15017_044) | 36052..36687 | - | 636 | WP_000934977 | A24 family peptidase | - |
| HTI99_RS00225 (pE15017_045) | 36691..37173 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTI99_RS00230 (pE15017_046) | 37239..37796 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| HTI99_RS00235 (pE15017_047) | 37841..38950 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTI99_RS00240 (pE15017_048) | 38941..40479 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTI99_RS00245 (pE15017_049) | 40504..40998 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTI99_RS00250 (pE15017_050) | 40982..42304 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTI99_RS00255 (pE15017_051) | 42343..43986 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| HTI99_RS00260 (pE15017_052) | 43979..44515 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HTI99_RS00265 (pE15017_053) | 44562..46520 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HTI99_RS00270 (pE15017_054) | 46536..47591 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTI99_RS00275 (pE15017_055) | 47610..48749 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| HTI99_RS00280 (pE15017_056) | 48739..49440 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTI99_RS00285 (pE15017_057) | 49506..50240 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTI99_RS00290 (pE15017_058) | 50406..52763 | - | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| HTI99_RS00295 (pE15017_059) | 52769..53089 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTI99_RS00435 (pE15017_060) | 53160..53450 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HTI99_RS00305 (pE15017_061) | 53450..54034 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| HTI99_RS00310 (pE15017_062) | 54055..54453 | - | 399 | WP_072643816 | hypothetical protein | - |
| HTI99_RS00315 (pE15017_063) | 54572..55009 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HTI99_RS00320 (pE15017_064) | 55015..56250 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| HTI99_RS00325 (pE15017_065) | 56253..56552 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTI99_RS00330 (pE15017_066) | 56620..56901 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTI99_RS00335 (pE15017_067) | 56891..57142 | - | 252 | WP_000121741 | hypothetical protein | - |
| HTI99_RS00340 (pE15017_068) | 57242..57877 | - | 636 | WP_015059536 | hypothetical protein | - |
| HTI99_RS00345 (pE15017_069) | 57950..58237 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| HTI99_RS00350 (pE15017_070) | 58250..58504 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| HTI99_RS00355 (pE15017_071) | 58506..59147 | - | 642 | WP_001425343 | type IV secretion system protein | - |
| HTI99_RS00360 (pE15017_072) | 59153..60148 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| HTI99_RS00365 (pE15017_073) | 60152..60409 | - | 258 | WP_000739144 | hypothetical protein | - |
| HTI99_RS00370 (pE15017_074) | 60406..60759 | - | 354 | WP_223286767 | hypothetical protein | - |
| HTI99_RS00375 (pE15017_076) | 60979..61425 | - | 447 | WP_001243165 | hypothetical protein | - |
| HTI99_RS00380 (pE15017_077) | 61436..61606 | - | 171 | WP_000550720 | hypothetical protein | - |
| HTI99_RS00385 (pE15017_078) | 61610..62053 | - | 444 | WP_000964330 | NfeD family protein | - |
| HTI99_RS00390 (pE15017_079) | 62427..63380 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| HTI99_RS00395 (pE15017_080) | 63407..63583 | - | 177 | WP_000753050 | hypothetical protein | - |
| HTI99_RS00400 (pE15017_081) | 63576..63791 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| HTI99_RS00405 (pE15017_082) | 63784..64290 | - | 507 | WP_001326595 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 3638 | GenBank | NZ_KX772778 |
| Plasmid name | pE15017_00 | Incompatibility group | IncI2 |
| Plasmid size | 65375 bp | Coordinate of oriT [Strand] | 18441..18493 [-] |
| Host baterium | Escherichia coli strain E15017_00 |
Cargo genes
| Drug resistance gene | blaCTX-M-55, mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |