Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103184 |
| Name | oriT_ZJ1635|unnamed  |
| Organism | Escherichia coli strain ZJ1635 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KX580716 (45756..45808 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_ZJ1635|unnamed
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2136 | GenBank | WP_015059539 |
| Name | t4cp2_HTI97_RS00065_ZJ1635|unnamed |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2248..25705
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTI97_RS00425 | 2..223 | + | 222 | Protein_1 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTI97_RS00015 | 220..1596 | - | 1377 | WP_000750519 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTI97_RS00020 | 1609..2244 | - | 636 | WP_000934977 | A24 family peptidase | - |
| HTI97_RS00025 | 2248..2730 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTI97_RS00030 | 2796..3353 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| HTI97_RS00035 | 3398..4507 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTI97_RS00040 | 4498..6036 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTI97_RS00045 | 6061..6555 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTI97_RS00050 | 6539..7861 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTI97_RS00055 | 7900..9543 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| HTI97_RS00060 | 9536..10072 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HTI97_RS00065 | 10119..12077 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HTI97_RS00070 | 12093..13148 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTI97_RS00075 | 13167..14306 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| HTI97_RS00080 | 14296..14997 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTI97_RS00085 | 15063..15797 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTI97_RS00090 | 15963..18320 | - | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| HTI97_RS00095 | 18326..18646 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTI97_RS00400 | 18717..19007 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HTI97_RS00105 | 19007..19591 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| HTI97_RS00110 | 19612..20010 | - | 399 | WP_001153665 | hypothetical protein | - |
| HTI97_RS00115 | 20129..20566 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HTI97_RS00120 | 20572..21807 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| HTI97_RS00125 | 21810..22109 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTI97_RS00130 | 22177..22458 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTI97_RS00135 | 22448..22699 | - | 252 | WP_000121741 | hypothetical protein | - |
| HTI97_RS00140 | 22799..23434 | - | 636 | WP_015059536 | hypothetical protein | - |
| HTI97_RS00145 | 23507..23794 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| HTI97_RS00150 | 23807..24061 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| HTI97_RS00155 | 24063..24704 | - | 642 | WP_001425343 | type IV secretion system protein | - |
| HTI97_RS00160 | 24710..25705 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| HTI97_RS00165 | 25709..25966 | - | 258 | WP_000739144 | hypothetical protein | - |
| HTI97_RS00170 | 25963..26316 | - | 354 | WP_223286767 | hypothetical protein | - |
| HTI97_RS00175 | 26536..26982 | - | 447 | WP_001243165 | hypothetical protein | - |
| HTI97_RS00180 | 26993..27163 | - | 171 | WP_000550720 | hypothetical protein | - |
| HTI97_RS00185 | 27167..27610 | - | 444 | WP_000964330 | NfeD family protein | - |
| HTI97_RS00190 | 27984..28937 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| HTI97_RS00195 | 28964..29140 | - | 177 | WP_000753050 | hypothetical protein | - |
| HTI97_RS00200 | 29133..29348 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| HTI97_RS00205 | 29341..29847 | - | 507 | WP_001326595 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 3627 | GenBank | NZ_KX580716 |
| Plasmid name | ZJ1635|unnamed | Incompatibility group | IncI2 |
| Plasmid size | 62440 bp | Coordinate of oriT [Strand] | 45756..45808 [-] |
| Host baterium | Escherichia coli strain ZJ1635 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |