Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103177 |
| Name | oriT_pKP301cro  |
| Organism | Klebsiella pneumoniae strain 301 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KY495890 (41631..41680 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKP301cro
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2378 | GenBank | WP_015065542 |
| Name | traI_HTL83_RS00065_pKP301cro |
UniProt ID | K7R3U7 |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190318.03 Da Isoelectric Point: 6.2890
>WP_015065542.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2126 | GenBank | WP_015065541 |
| Name | traD_HTL83_RS00070_pKP301cro |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 86025.00 Da Isoelectric Point: 5.0577
>WP_015065541.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 12211..42238
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTL83_RS00070 | 12211..14523 | - | 2313 | WP_015065541 | type IV conjugative transfer system coupling protein TraD | virb4 |
| HTL83_RS00075 | 14652..15341 | - | 690 | WP_072198073 | hypothetical protein | - |
| HTL83_RS00080 | 15534..16265 | - | 732 | WP_016831056 | conjugal transfer complement resistance protein TraT | - |
| HTL83_RS00085 | 16516..17118 | - | 603 | WP_071604857 | hypothetical protein | - |
| HTL83_RS00090 | 17141..19963 | - | 2823 | WP_016831055 | conjugal transfer mating-pair stabilization protein TraG | traG |
| HTL83_RS00095 | 19963..21342 | - | 1380 | WP_072159542 | conjugal transfer pilus assembly protein TraH | traH |
| HTL83_RS00100 | 21320..21763 | - | 444 | WP_016831053 | F-type conjugal transfer protein TrbF | - |
| HTL83_RS00105 | 21809..22366 | - | 558 | WP_004152678 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| HTL83_RS00110 | 22338..22577 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| HTL83_RS00115 | 22588..23340 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| HTL83_RS00120 | 23361..23687 | - | 327 | WP_016831051 | hypothetical protein | - |
| HTL83_RS00125 | 23700..23948 | - | 249 | WP_004152675 | hypothetical protein | - |
| HTL83_RS00130 | 23926..24180 | - | 255 | WP_015065558 | conjugal transfer protein TrbE | - |
| HTL83_RS00135 | 24212..26167 | - | 1956 | WP_023313940 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| HTL83_RS00140 | 26226..26873 | - | 648 | WP_039109927 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| HTL83_RS00145 | 27149..28138 | - | 990 | WP_015632509 | conjugal transfer pilus assembly protein TraU | traU |
| HTL83_RS00150 | 28135..28536 | - | 402 | WP_016831047 | hypothetical protein | - |
| HTL83_RS00155 | 28571..29206 | - | 636 | WP_016831046 | type-F conjugative transfer system protein TraW | traW |
| HTL83_RS00160 | 29206..29595 | - | 390 | WP_004167468 | type-F conjugative transfer system protein TrbI | - |
| HTL83_RS00165 | 29595..32234 | - | 2640 | WP_016831045 | type IV secretion system protein TraC | virb4 |
| HTL83_RS00170 | 32306..32704 | - | 399 | WP_023158008 | hypothetical protein | - |
| HTL83_RS00175 | 32712..33005 | - | 294 | WP_020325117 | hypothetical protein | - |
| HTL83_RS00180 | 33145..33549 | - | 405 | WP_016831042 | hypothetical protein | - |
| HTL83_RS00185 | 33616..33927 | - | 312 | WP_016831041 | hypothetical protein | - |
| HTL83_RS00190 | 33928..34146 | - | 219 | WP_014386199 | hypothetical protein | - |
| HTL83_RS00195 | 34170..34459 | - | 290 | Protein_37 | hypothetical protein | - |
| HTL83_RS00200 | 34464..34874 | - | 411 | WP_032409654 | hypothetical protein | - |
| HTL83_RS00205 | 35006..35590 | - | 585 | WP_014386196 | type IV conjugative transfer system lipoprotein TraV | traV |
| HTL83_RS00210 | 35613..36209 | - | 597 | Protein_40 | conjugal transfer pilus-stabilizing protein TraP | - |
| HTL83_RS00215 | 36202..37626 | - | 1425 | WP_016831036 | F-type conjugal transfer pilus assembly protein TraB | traB |
| HTL83_RS00220 | 37626..38366 | - | 741 | WP_032408905 | type-F conjugative transfer system secretin TraK | traK |
| HTL83_RS00225 | 38353..38919 | - | 567 | WP_016831035 | type IV conjugative transfer system protein TraE | traE |
| HTL83_RS00230 | 38939..39244 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| HTL83_RS00235 | 39258..39626 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| HTL83_RS00240 | 39688..39894 | - | 207 | WP_171773970 | TraY domain-containing protein | - |
| HTL83_RS00245 | 40027..40746 | - | 720 | WP_016831034 | conjugal transfer protein TrbJ | - |
| HTL83_RS00250 | 40946..41362 | - | 417 | WP_072145360 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| HTL83_RS00255 | 41753..42238 | + | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| HTL83_RS00260 | 42271..42600 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| HTL83_RS00265 | 42633..43454 | - | 822 | WP_004182076 | DUF932 domain-containing protein | - |
| HTL83_RS00270 | 44082..44432 | - | 351 | WP_004182068 | hypothetical protein | - |
| HTL83_RS00275 | 44429..44701 | - | 273 | WP_004182067 | hypothetical protein | - |
| HTL83_RS00825 | 45397..45558 | - | 162 | WP_004118124 | type I toxin-antitoxin system Hok family toxin | - |
| HTL83_RS00285 | 45630..46709 | + | 1080 | WP_004220208 | IS481-like element ISKpn28 family transposase | - |
Host bacterium
| ID | 3620 | GenBank | NZ_KY495890 |
| Plasmid name | pKP301cro | Incompatibility group | IncFII |
| Plasmid size | 147442 bp | Coordinate of oriT [Strand] | 41631..41680 [+] |
| Host baterium | Klebsiella pneumoniae strain 301 |
Cargo genes
| Drug resistance gene | blaCTX-M-15 |
| Virulence gene | - |
| Metal resistance gene | silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |