Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103175 |
| Name | oriT_pOXA-519  |
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain Kp1210 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KY215945 (31882..31987 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pOXA-519
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2376 | GenBank | WP_004187323 |
| Name | Relaxase_HTK89_RS00265_pOXA-519 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 2124 | GenBank | WP_004206885 |
| Name | t4cp2_HTK89_RS00385_pOXA-519 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34783..60665
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTK89_RS00245 | 29867..30178 | + | 312 | WP_004187333 | hypothetical protein | - |
| HTK89_RS00250 | 30311..30847 | + | 537 | WP_004187332 | hypothetical protein | - |
| HTK89_RS00255 | 31349..31744 | - | 396 | WP_019725163 | hypothetical protein | - |
| HTK89_RS00400 | 31743..32306 | + | 564 | WP_020277892 | plasmid mobilization protein MobA | - |
| HTK89_RS00265 | 32293..34272 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| HTK89_RS00270 | 34286..34786 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| HTK89_RS00275 | 34783..35562 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| HTK89_RS00280 | 35573..36736 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| HTK89_RS00285 | 36726..36986 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| HTK89_RS00290 | 37011..38363 | + | 1353 | WP_172689443 | toprim domain-containing protein | - |
| HTK89_RS00295 | 38326..40305 | + | 1980 | WP_228719201 | LPD7 domain-containing protein | - |
| HTK89_RS00300 | 40271..40783 | + | 513 | WP_011091071 | hypothetical protein | traL |
| HTK89_RS00305 | 40784..40981 | - | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| HTK89_RS00310 | 40968..41378 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| HTK89_RS00315 | 41377..42159 | + | 783 | WP_015058941 | DotI/IcmL family type IV secretion protein | traM |
| HTK89_RS00320 | 42168..43319 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| HTK89_RS00325 | 43331..44680 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| HTK89_RS00330 | 44692..45396 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| HTK89_RS00335 | 45420..45950 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| HTK89_RS00340 | 45967..46356 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| HTK89_RS00345 | 46402..46896 | + | 495 | WP_004187480 | hypothetical protein | - |
| HTK89_RS00350 | 46893..49943 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| HTK89_RS00355 | 49940..51148 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| HTK89_RS00360 | 51145..51741 | + | 597 | WP_015060003 | hypothetical protein | - |
| HTK89_RS00365 | 51734..53929 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| HTK89_RS00370 | 53931..54584 | + | 654 | WP_015060005 | hypothetical protein | - |
| HTK89_RS00375 | 54653..54895 | + | 243 | WP_023893611 | IncL/M type plasmid replication protein RepC | - |
| HTK89_RS00380 | 55179..56246 | + | 1068 | WP_019725043 | plasmid replication initiator RepA | - |
| HTK89_RS00405 | 57469..57564 | + | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| HTK89_RS00385 | 57615..59702 | - | 2088 | WP_004206885 | conjugal transfer protein TrbC | - |
| HTK89_RS00390 | 59715..60665 | - | 951 | WP_004206886 | DsbC family protein | trbB |
Host bacterium
| ID | 3618 | GenBank | NZ_KY215945 |
| Plasmid name | pOXA-519 | Incompatibility group | IncL/M |
| Plasmid size | 60678 bp | Coordinate of oriT [Strand] | 31882..31987 [+] |
| Host baterium | Klebsiella pneumoniae subsp. pneumoniae strain Kp1210 |
Cargo genes
| Drug resistance gene | blaOXA-519 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA8 |