Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103172
• Name: oriT_pOXA48_wz
• Organism: Klebsiella pneumoniae strain 38_wz
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_KY213890 (33815..33920 [+], 106 nt)
• oriT length: 106 nt
• IRs (inverted repeats): 30..35, 41..46 (CCGCCG..CGGCGG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 106 nt

>oriT_pOXA48_wz
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_HTK56_RS00250_pOXA48_wz

• ID: 2374
• GenBank: WP_004187323
• Name: Relaxase_HTK56_RS00250_pOXA48_wz
• UniProt ID: A0A3U8KUL3
• Length: 659 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 659 a.a.
• Molecular weight: 75179.89 Da
• Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (86-334)

Protein structure

• AlphaFold DB: A0A3U8KUL3

T4CP

t4cp2_HTK56_RS00015_pOXA48_wz

• ID: 2120
• GenBank: WP_004206885
• Name: t4cp2_HTK56_RS00015_pOXA48_wz
• UniProt ID: _
• Length: 695 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 695 a.a.
• Molecular weight: 79538.91 Da
• Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

Protein domains

No domain identified.

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 36716..55915

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HTK56_RS00230	31800..32111	+	312	WP_004187333	hypothetical protein	-
HTK56_RS00235	32244..32780	+	537	WP_004187332	hypothetical protein	-
HTK56_RS00240	33282..33677	-	396	WP_019725163	hypothetical protein	-
HTK56_RS00360	33676..34239	+	564	WP_020277892	plasmid mobilization protein MobA	-
HTK56_RS00250	34226..36205	+	1980	WP_004187323	TraI/MobA(P) family conjugative relaxase	-
HTK56_RS00255	36219..36719	+	501	WP_004187320	DotD/TraH family lipoprotein	-
HTK56_RS00260	36716..37495	+	780	WP_004187315	type IV secretory system conjugative DNA transfer family protein	traI
HTK56_RS00265	37506..38669	+	1164	WP_004187313	plasmid transfer ATPase TraJ	virB11
HTK56_RS00270	38659..38919	+	261	WP_004187310	IcmT/TraK family protein	traK
HTK56_RS00365	38944..42291	+	3348	WP_223807062	LPD7 domain-containing protein	-
HTK56_RS00280	42257..42769	+	513	WP_011091071	hypothetical protein	traL
HTK56_RS00285	42770..42967	-	198	WP_004187464	Hha/YmoA family nucleoid-associated regulatory protein	-
HTK56_RS00290	42954..43364	-	411	WP_004187465	H-NS family nucleoid-associated regulatory protein	-
HTK56_RS00295	43363..44145	+	783	WP_015058941	DotI/IcmL family type IV secretion protein	traM
HTK56_RS00300	44154..45305	+	1152	WP_004187471	DotH/IcmK family type IV secretion protein	traN
HTK56_RS00305	45317..46666	+	1350	WP_004187474	conjugal transfer protein TraO	traO
HTK56_RS00310	46678..47382	+	705	WP_015060002	conjugal transfer protein TraP	traP
HTK56_RS00315	47406..47936	+	531	WP_004187478	conjugal transfer protein TraQ	traQ
HTK56_RS00320	47953..48342	+	390	WP_004187479	DUF6750 family protein	traR
HTK56_RS00325	48388..48882	+	495	WP_004187480	hypothetical protein	-
HTK56_RS00330	48879..51929	+	3051	WP_004187482	hypothetical protein	traU
HTK56_RS00335	51926..53134	+	1209	WP_011091082	conjugal transfer protein TraW	traW
HTK56_RS00340	53131..53727	+	597	WP_015060003	hypothetical protein	-
HTK56_RS00345	53720..55915	+	2196	WP_015062834	DotA/TraY family protein	traY
HTK56_RS00350	55917..56570	+	654	WP_015060005	hypothetical protein	-

Host bacterium

• ID: 3615
• GenBank: NZ_KY213890
• Plasmid name: pOXA48_wz
• Incompatibility group: IncL/M
• Plasmid size: 56650 bp
• Coordinate of oriT [Strand]: 33815..33920 [+]
• Host baterium: Klebsiella pneumoniae strain 38_wz

Cargo genes

• Drug resistance gene: blaOXA-48
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -