Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103171 |
| Name | oriT_pCRKP-5-KPC  |
| Organism | Klebsiella pneumoniae strain CRKP-5-KPC |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KX928751 (47145..47245 [-], 101 nt) |
| oriT length | 101 nt |
| IRs (inverted repeats) | 80..85, 91..96 (AAAAAA..TTTTTT) 20..26, 38..44 (TAAATCA..TGATTTA) |
| Location of nic site | 62..63 |
| Conserved sequence flanking the nic site |
GGTGTATAGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 101 nt
>oriT_pCRKP-5-KPC
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2373 | GenBank | WP_250645733 |
| Name | TrwC_HTK43_RS00460_pCRKP-5-KPC |
UniProt ID | _ |
| Length | 607 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 607 a.a. Molecular weight: 67669.66 Da Isoelectric Point: 9.7241
>WP_250645733.1 MobF family relaxase [Klebsiella pneumoniae]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGGYVTDESGRSR
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGGYVTDESGRSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 1003 | GenBank | WP_001749975 |
| Name | WP_001749975_pCRKP-5-KPC |
UniProt ID | A0A5C2CVS6 |
| Length | 138 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 138 a.a. Molecular weight: 15332.60 Da Isoelectric Point: 5.9670
>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5C2CVS6 |
T4CP
| ID | 2119 | GenBank | WP_000342688 |
| Name | t4cp2_HTK43_RS00315_pCRKP-5-KPC |
UniProt ID | _ |
| Length | 509 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 509 a.a. Molecular weight: 57762.87 Da Isoelectric Point: 9.6551
>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 21865..31159
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTK43_RS00100 | 17336..18730 | + | 1395 | Protein_19 | ISKra4-like element ISKpn19 family transposase | - |
| HTK43_RS00105 | 18889..19586 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| HTK43_RS00480 | 19829..20116 | + | 288 | Protein_21 | DUF6710 family protein | - |
| HTK43_RS00115 | 20290..20827 | - | 538 | Protein_22 | phospholipase D family protein | - |
| HTK43_RS00120 | 20827..21823 | - | 997 | Protein_23 | ATPase, T2SS/T4P/T4SS family | - |
| HTK43_RS00125 | 21865..23025 | - | 1161 | WP_172688988 | type IV secretion system protein VirB10 | virB10 |
| HTK43_RS00130 | 23025..23909 | - | 885 | WP_000735066 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| HTK43_RS00135 | 23920..24618 | - | 699 | WP_000646594 | type IV secretion system protein | virB8 |
| HTK43_RS00140 | 24608..24766 | - | 159 | WP_012561180 | hypothetical protein | - |
| HTK43_RS00145 | 24837..25697 | - | 861 | WP_228719434 | type IV secretion system protein | - |
| HTK43_RS00420 | 25694..25879 | - | 186 | WP_228719430 | hypothetical protein | virB6 |
| HTK43_RS00150 | 25895..26122 | - | 228 | WP_001749959 | IncN-type entry exclusion lipoprotein EexN | - |
| HTK43_RS00155 | 26130..26894 | - | 765 | WP_013149461 | type IV secretion system protein | virB5 |
| HTK43_RS00425 | 26861..27409 | - | 549 | WP_227543278 | hypothetical protein | virb4 |
| HTK43_RS00430 | 27406..27915 | - | 510 | WP_228719431 | hypothetical protein | virb4 |
| HTK43_RS00435 | 28079..28642 | - | 564 | WP_228719432 | hypothetical protein | virb4 |
| HTK43_RS00440 | 28609..29463 | - | 855 | WP_228719433 | hypothetical protein | virb4 |
| HTK43_RS00165 | 29463..29780 | - | 318 | WP_000496058 | VirB3 family type IV secretion system protein | virB3 |
| HTK43_RS00170 | 29832..30125 | - | 294 | WP_001749962 | hypothetical protein | virB2 |
| HTK43_RS00175 | 30135..30416 | - | 282 | WP_000440698 | transcriptional repressor KorA | - |
| HTK43_RS00180 | 30425..31159 | - | 735 | WP_001749963 | lytic transglycosylase domain-containing protein | virB1 |
| HTK43_RS00185 | 31202..31573 | + | 372 | WP_011867773 | H-NS family nucleoid-associated regulatory protein | - |
| HTK43_RS00190 | 31589..31933 | + | 345 | WP_001749964 | hypothetical protein | - |
| HTK43_RS00195 | 31930..32244 | + | 315 | WP_001749965 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTK43_RS00200 | 32358..32591 | + | 234 | WP_001191790 | hypothetical protein | - |
| HTK43_RS00205 | 32641..33288 | + | 648 | WP_015344958 | restriction endonuclease | - |
| HTK43_RS00210 | 33293..33499 | + | 207 | WP_001749967 | hypothetical protein | - |
| HTK43_RS00215 | 33510..33782 | - | 273 | Protein_46 | IS1 family transposase | - |
| HTK43_RS00220 | 33881..35314 | + | 1434 | WP_001288432 | DNA cytosine methyltransferase | - |
Host bacterium
| ID | 3614 | GenBank | NZ_KX928751 |
| Plasmid name | pCRKP-5-KPC | Incompatibility group | IncN |
| Plasmid size | 68482 bp | Coordinate of oriT [Strand] | 47145..47245 [-] |
| Host baterium | Klebsiella pneumoniae strain CRKP-5-KPC |
Cargo genes
| Drug resistance gene | blaKPC-2, ARR-3, aac(6')-Ib-cr, qnrS1, dfrA14, blaCTX-M-3, blaTEM-1B |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |