Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103170
Name   oriT_pKP1814-2 in_silico
Organism   Klebsiella pneumoniae strain KP1814
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KX839208 (52297..52346 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  GGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pKP1814-2
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2372 GenBank   WP_023307532
Name   traI_HTK02_RS00490_pKP1814-2 insolico UniProt ID   A0A2R4ND60
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190476.66 Da        Isoelectric Point: 6.4600

>WP_023307532.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRAMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELACAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRHNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALITIEQVTPEQVPRKEGVWAPGSSVVEFTPKQEKAIE
KALSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRTLNGMIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSDGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASERYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGNSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNDAARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRIWADPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1433-1555)

AAA domain (AAA_30)

(971-1154)

TrwC relaxase (TrwC)

(10-284)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)


  Protein structure



No available structure.




T4CP

ID   2118 GenBank   WP_015065643
Name   traD_HTK02_RS00485_pKP1814-2 insolico UniProt ID   _
Length   769 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 769 a.a.        Molecular weight: 85851.84 Da        Isoelectric Point: 5.0577

>WP_015065643.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTVWCGEQLWTSIVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRRLDTRVDARLSALLEAREAEGSLARTLFMPDAPASGPADTNSHAGEQPEPISQPA
PADMTVSPAPVKAPPTTKSPAAEPSVRATEPPVLRVTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 51739..80220

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTK02_RS00260 46758..47108 + 351 WP_023280874 hypothetical protein -
HTK02_RS00265 47745..48101 + 357 WP_004152717 hypothetical protein -
HTK02_RS00270 48162..48374 + 213 WP_004152718 hypothetical protein -
HTK02_RS00275 48385..48609 + 225 WP_004152719 hypothetical protein -
HTK02_RS00280 48690..49010 + 321 WP_004152720 type II toxin-antitoxin system RelE/ParE family toxin -
HTK02_RS00285 49000..49278 + 279 WP_004152721 helix-turn-helix transcriptional regulator -
HTK02_RS00290 49279..49692 + 414 WP_023280875 transcriptional regulator -
HTK02_RS00295 50523..51350 + 828 WP_023307552 DUF932 domain-containing protein -
HTK02_RS00300 51377..51706 + 330 WP_011977736 DUF5983 family protein -
HTK02_RS00305 51739..52224 - 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
HTK02_RS00310 52659..53051 + 393 WP_032436755 conjugal transfer relaxosome DNA-binding protein TraM -
HTK02_RS00315 53267..53953 + 687 WP_228717881 transcriptional regulator TraJ family protein -
HTK02_RS00320 54037..54408 + 372 WP_004208838 TraY domain-containing protein -
HTK02_RS00325 54462..54830 + 369 WP_023307550 type IV conjugative transfer system pilin TraA -
HTK02_RS00330 54844..55149 + 306 WP_004178059 type IV conjugative transfer system protein TraL traL
HTK02_RS00335 55169..55735 + 567 WP_004152602 type IV conjugative transfer system protein TraE traE
HTK02_RS00340 55722..56462 + 741 WP_004152497 type-F conjugative transfer system secretin TraK traK
HTK02_RS00345 56462..57886 + 1425 WP_023307549 F-type conjugal transfer pilus assembly protein TraB traB
HTK02_RS00350 57879..58292 + 414 Protein_75 conjugal transfer pilus-stabilizing protein TraP -
HTK02_RS00355 58308..58484 + 177 WP_172687660 hypothetical protein -
HTK02_RS00360 58504..59073 + 570 WP_023307547 type IV conjugative transfer system lipoprotein TraV traV
HTK02_RS00365 59205..59615 + 411 WP_023307546 hypothetical protein -
HTK02_RS00370 59620..59910 + 291 WP_172687661 hypothetical protein -
HTK02_RS00375 59934..60152 + 219 WP_023307545 hypothetical protein -
HTK02_RS00380 60153..60470 + 318 WP_023307544 hypothetical protein -
HTK02_RS00385 60537..60941 + 405 WP_023307543 hypothetical protein -
HTK02_RS00390 60938..61228 + 291 WP_023307542 hypothetical protein -
HTK02_RS00395 61236..61634 + 399 WP_072158599 hypothetical protein -
HTK02_RS00400 61706..64345 + 2640 WP_016528865 type IV secretion system protein TraC virb4
HTK02_RS00405 64345..64734 + 390 WP_004152591 type-F conjugative transfer system protein TrbI -
HTK02_RS00410 64734..65360 + 627 WP_023307538 type-F conjugative transfer system protein TraW traW
HTK02_RS00415 65404..66363 + 960 WP_015065634 conjugal transfer pilus assembly protein TraU traU
HTK02_RS00420 66376..67014 + 639 WP_015065635 type-F conjugative transfer system pilin assembly protein TrbC trbC
HTK02_RS00425 67073..69028 + 1956 WP_023307537 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HTK02_RS00430 69060..69296 + 237 WP_023307536 conjugal transfer protein TrbE -
HTK02_RS01055 69293..69478 + 186 WP_023307535 hypothetical protein -
HTK02_RS00435 69524..69850 + 327 WP_004152685 hypothetical protein -
HTK02_RS00440 69871..70623 + 753 WP_015065637 type-F conjugative transfer system pilin assembly protein TraF traF
HTK02_RS00445 70634..70873 + 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
HTK02_RS00450 70845..71402 + 558 WP_004152678 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HTK02_RS00455 71447..71890 + 444 WP_015065638 F-type conjugal transfer protein TrbF -
HTK02_RS00460 71868..73247 + 1380 WP_011977731 conjugal transfer pilus assembly protein TraH traH
HTK02_RS00465 73247..76096 + 2850 WP_108479757 conjugal transfer mating-pair stabilization protein TraG traG
HTK02_RS00470 76102..76677 + 576 WP_064187086 hypothetical protein -
HTK02_RS00480 76818..77549 + 732 Protein_101 conjugal transfer complement resistance protein TraT -
HTK02_RS00485 77911..80220 + 2310 WP_015065643 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   3613 GenBank   NZ_KX839208
Plasmid name   pKP1814-2 Incompatibility group   IncFII
Plasmid size   187349 bp Coordinate of oriT [Strand]   52297..52346 [-]
Host baterium   Klebsiella pneumoniae strain KP1814

Cargo genes

Drug resistance gene   -
Virulence gene   mrkJ, mrkF, mrkD, mrkC, mrkB, mrkA
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9