Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103162
Name   oriT_pKP4368 in_silico
Organism   Klebsiella pneumoniae strain KP4368
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KX783441 (18343..18447 [-], 105 nt)
oriT length   105 nt
IRs (inverted repeats)      32..37, 40..45  (GCCGGC..GCCGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 105 nt

>oriT_pKP4368
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2365 GenBank   WP_004206924
Name   Relaxase_HTJ41_RS00090_pKP4368 insolico UniProt ID   A0A2V4FJ40
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75087.84 Da        Isoelectric Point: 10.0285

>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A2V4FJ40


T4CP

ID   2107 GenBank   WP_064217614
Name   t4cp2_HTJ41_RS00380_pKP4368 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79489.84 Da        Isoelectric Point: 4.8290

>WP_064217614.1 MULTISPECIES: hypothetical protein [Enterobacteriaceae]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRDRKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLAAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 374..15545

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTJ41_RS00010 (pKP4368_0001) 374..3424 - 3051 WP_011154474 conjugative transfer protein traU
HTJ41_RS00015 (pKP4368_0002) 3421..3915 - 495 WP_004187480 hypothetical protein -
HTJ41_RS00020 (pKP4368_0003) 3961..4350 - 390 WP_011154472 DUF6750 family protein traR
HTJ41_RS00025 (pKP4368_0004) 4367..4897 - 531 WP_004187478 conjugal transfer protein TraQ traQ
HTJ41_RS00030 (pKP4368_0005) 4921..5625 - 705 WP_004206933 conjugal transfer protein TraP traP
HTJ41_RS00035 (pKP4368_0006) 5637..6986 - 1350 WP_004206932 conjugal transfer protein TraO traO
HTJ41_RS00040 (pKP4368_0007) 6998..8149 - 1152 WP_015062843 DotH/IcmK family type IV secretion protein traN
HTJ41_RS00045 (pKP4368_0008) 8158..8940 - 783 WP_004206929 DotI/IcmL family type IV secretion protein traM
HTJ41_RS00050 8939..9349 + 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
HTJ41_RS00055 9336..9533 + 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
HTJ41_RS00060 (pKP4368_0010) 9534..10088 - 555 WP_011154467 hypothetical protein traL
HTJ41_RS00420 (pKP4368_0011) 10012..13317 - 3306 WP_219818010 LPD7 domain-containing protein -
HTJ41_RS00070 13342..13602 - 261 WP_004187310 IcmT/TraK family protein traK
HTJ41_RS00075 (pKP4368_0012) 13592..14755 - 1164 WP_004206926 plasmid transfer ATPase TraJ virB11
HTJ41_RS00080 (pKP4368_0013) 14766..15545 - 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
HTJ41_RS00085 (pKP4368_0014) 15542..16042 - 501 WP_004206925 DotD/TraH family lipoprotein -
HTJ41_RS00090 (pKP4368_0015) 16056..18035 - 1980 WP_004206924 TraI/MobA(P) family conjugative relaxase -
HTJ41_RS00095 (pKP4368_0016) 18022..18339 - 318 WP_004206923 plasmid mobilization protein MobA -
HTJ41_RS00100 (pKP4368_0017) 18615..18980 + 366 WP_004206922 hypothetical protein -
HTJ41_RS00105 (pKP4368_0018) 19481..20017 - 537 WP_004206920 hypothetical protein -
HTJ41_RS00110 (pKP4368_0019) 20150..20461 - 312 WP_004187333 hypothetical protein -

Host bacterium

ID   3605 GenBank   NZ_KX783441
Plasmid name   pKP4368 Incompatibility group   IncL/M
Plasmid size   67144 bp Coordinate of oriT [Strand]   18343..18447 [-]
Host baterium   Klebsiella pneumoniae strain KP4368

Cargo genes

Drug resistance gene   blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -