Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103156 |
| Name | oriT_pOXA-48_30891  |
| Organism | Klebsiella pneumoniae strain Kpn-30891/15 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KX523902 (37123..37228 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pOXA-48_30891
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2361 | GenBank | WP_004187323 |
| Name | Relaxase_HTI96_RS00310_pOXA-48_30891 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 2100 | GenBank | WP_004206885 |
| Name | t4cp2_HTI96_RS00425_pOXA-48_30891 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 40024..66049
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTI96_RS00290 | 35108..35419 | + | 312 | WP_004187333 | hypothetical protein | - |
| HTI96_RS00295 | 35552..36088 | + | 537 | WP_004187332 | hypothetical protein | - |
| HTI96_RS00300 | 36590..36985 | - | 396 | WP_019725163 | hypothetical protein | - |
| HTI96_RS00440 | 36984..37547 | + | 564 | WP_020277892 | plasmid mobilization protein MobA | - |
| HTI96_RS00310 | 37534..39513 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| HTI96_RS00315 | 39527..40027 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| HTI96_RS00320 | 40024..40803 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| HTI96_RS00325 | 40814..41977 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| HTI96_RS00330 | 41967..42227 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| HTI96_RS00445 | 42252..45689 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| HTI96_RS00340 | 45655..46167 | + | 513 | WP_011091071 | hypothetical protein | traL |
| HTI96_RS00345 | 46168..46365 | - | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| HTI96_RS00350 | 46352..46762 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| HTI96_RS00355 | 46761..47543 | + | 783 | WP_172687519 | DotI/IcmL family type IV secretion protein | traM |
| HTI96_RS00360 | 47552..48703 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| HTI96_RS00365 | 48715..50064 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| HTI96_RS00370 | 50076..50780 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| HTI96_RS00375 | 50804..51334 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| HTI96_RS00380 | 51351..51740 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| HTI96_RS00385 | 51786..52280 | + | 495 | WP_004187480 | hypothetical protein | - |
| HTI96_RS00390 | 52277..55327 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| HTI96_RS00395 | 55324..56532 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| HTI96_RS00400 | 56529..57125 | + | 597 | WP_015060003 | hypothetical protein | - |
| HTI96_RS00405 | 57118..59313 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| HTI96_RS00410 | 59315..59968 | + | 654 | WP_015060005 | hypothetical protein | - |
| HTI96_RS00415 | 60037..60279 | + | 243 | WP_023893611 | IncL/M type plasmid replication protein RepC | - |
| HTI96_RS00420 | 60563..61630 | + | 1068 | WP_019725043 | plasmid replication initiator RepA | - |
| HTI96_RS00450 | 62853..62948 | + | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| HTI96_RS00425 | 62999..65086 | - | 2088 | WP_004206885 | conjugal transfer protein TrbC | - |
| HTI96_RS00430 | 65099..66049 | - | 951 | WP_004206886 | DsbC family protein | trbB |
Host bacterium
| ID | 3599 | GenBank | NZ_KX523902 |
| Plasmid name | pOXA-48_30891 | Incompatibility group | IncL/M |
| Plasmid size | 66059 bp | Coordinate of oriT [Strand] | 37123..37228 [+] |
| Host baterium | Klebsiella pneumoniae strain Kpn-30891/15 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |