Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103148
• Name: oriT_pOXA-48_4963
• Organism: Klebsiella pneumoniae strain Kpn-04963/15
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_KX523900 (34630..34735 [+], 106 nt)
• oriT length: 106 nt
• IRs (inverted repeats): 30..35, 41..46 (CCGCCG..CGGCGG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 106 nt

>oriT_pOXA-48_4963
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_HTI94_RS00295_pOXA-48_4963

• ID: 2358
• GenBank: WP_004187323
• Name: Relaxase_HTI94_RS00295_pOXA-48_4963
• UniProt ID: A0A3U8KUL3
• Length: 659 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 659 a.a.
• Molecular weight: 75179.89 Da
• Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (86-334)

Protein structure

• AlphaFold DB: A0A3U8KUL3

T4CP

t4cp2_HTI94_RS00410_pOXA-48_4963

• ID: 2093
• GenBank: WP_004206885
• Name: t4cp2_HTI94_RS00410_pOXA-48_4963
• UniProt ID: _
• Length: 695 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 695 a.a.
• Molecular weight: 79538.91 Da
• Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

Protein domains

No domain identified.

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 37531..63556

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HTI94_RS00275	32615..32926	+	312	WP_004187333	hypothetical protein	-
HTI94_RS00280	33059..33595	+	537	WP_004187332	hypothetical protein	-
HTI94_RS00285	34097..34492	-	396	WP_019725163	hypothetical protein	-
HTI94_RS00425	34491..35054	+	564	WP_020277892	plasmid mobilization protein MobA	-
HTI94_RS00295	35041..37020	+	1980	WP_004187323	TraI/MobA(P) family conjugative relaxase	-
HTI94_RS00300	37034..37534	+	501	WP_004187320	DotD/TraH family lipoprotein	-
HTI94_RS00305	37531..38310	+	780	WP_004187315	type IV secretory system conjugative DNA transfer family protein	traI
HTI94_RS00310	38321..39484	+	1164	WP_004187313	plasmid transfer ATPase TraJ	virB11
HTI94_RS00315	39474..39734	+	261	WP_004187310	IcmT/TraK family protein	traK
HTI94_RS00430	39759..43196	+	3438	WP_228719122	LPD7 domain-containing protein	-
HTI94_RS00325	43162..43674	+	513	WP_011091071	hypothetical protein	traL
HTI94_RS00330	43675..43872	-	198	WP_004187464	Hha/YmoA family nucleoid-associated regulatory protein	-
HTI94_RS00335	43859..44269	-	411	WP_004187465	H-NS family nucleoid-associated regulatory protein	-
HTI94_RS00340	44268..45050	+	783	WP_015058941	DotI/IcmL family type IV secretion protein	traM
HTI94_RS00345	45059..46210	+	1152	WP_004187471	DotH/IcmK family type IV secretion protein	traN
HTI94_RS00350	46222..47571	+	1350	WP_004187474	conjugal transfer protein TraO	traO
HTI94_RS00355	47583..48287	+	705	WP_015060002	conjugal transfer protein TraP	traP
HTI94_RS00360	48311..48841	+	531	WP_004187478	conjugal transfer protein TraQ	traQ
HTI94_RS00365	48858..49247	+	390	WP_004187479	DUF6750 family protein	traR
HTI94_RS00370	49293..49787	+	495	WP_004187480	hypothetical protein	-
HTI94_RS00375	49784..52834	+	3051	WP_004187482	hypothetical protein	traU
HTI94_RS00380	52831..54039	+	1209	WP_011091082	conjugal transfer protein TraW	traW
HTI94_RS00385	54036..54632	+	597	WP_015060003	hypothetical protein	-
HTI94_RS00390	54625..56820	+	2196	WP_015062834	DotA/TraY family protein	traY
HTI94_RS00395	56822..57475	+	654	WP_015060005	hypothetical protein	-
HTI94_RS00400	57544..57786	+	243	WP_023893611	IncL/M type plasmid replication protein RepC	-
HTI94_RS00405	58070..59137	+	1068	WP_019725043	plasmid replication initiator RepA	-
HTI94_RS00435	60360..60455	+	96	WP_004206884	DinQ-like type I toxin DqlB	-
HTI94_RS00410	60506..62593	-	2088	WP_004206885	conjugal transfer protein TrbC	-
HTI94_RS00415	62606..63556	-	951	WP_004206886	DsbC family protein	trbB

Host bacterium

• ID: 3591
• GenBank: NZ_KX523900
• Plasmid name: pOXA-48_4963
• Incompatibility group: IncL/M
• Plasmid size: 63566 bp
• Coordinate of oriT [Strand]: 34630..34735 [+]
• Host baterium: Klebsiella pneumoniae strain Kpn-04963/15

Cargo genes

• Drug resistance gene: blaOXA-48
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -