Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103146
• Name: oriT_pBA01611
• Organism: Staphylococcus aureus strain BA01611
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_KY662247 (1446..1483 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_pBA01611
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_Pre_HTM03_RS00005_pBA01611

• ID: 2356
• GenBank: WP_031586516
• Name: Mob_Pre_HTM03_RS00005_pBA01611
• UniProt ID: A0A1V0CV19
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49672.38 Da
• Isoelectric Point: 9.9693

>WP_031586516.1 MULTISPECIES: MobV family relaxase [Staphylococcus]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYAIVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: A0A1V0CV19

Host bacterium

• ID: 3589
• GenBank: NZ_KY662247
• Plasmid name: pBA01611
• Incompatibility group: -
• Plasmid size: 7440 bp
• Coordinate of oriT [Strand]: 1446..1483 [-]
• Host baterium: Staphylococcus aureus strain BA01611

Cargo genes

• Drug resistance gene: tet(L), aadD
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -