Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103139
Name   oriT_pS10584 in_silico
Organism   Salmonella enterica strain SJTUF10584
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KX058576 (40824..40912 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pS10584
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2354 GenBank   WP_010891268
Name   nikB_HTI69_RS00295_pS10584 insolico UniProt ID   A0A632VGA6
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103977.42 Da        Isoelectric Point: 7.2753

>WP_010891268.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYIAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A632VGA6


Auxiliary protein

ID   996 GenBank   WP_001283947
Name   WP_001283947_pS10584 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2087 GenBank   WP_001289276
Name   trbC_HTI69_RS00300_pS10584 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 46290..84590

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTI69_RS00300 44006..46297 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
HTI69_RS00305 46290..47360 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
HTI69_RS00310 47379..48587 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HTI69_RS00315 48879..49031 + 153 WP_001331364 Hok/Gef family protein -
HTI69_RS00320 49103..49354 - 252 WP_001291964 hypothetical protein -
HTI69_RS00575 49853..49948 + 96 WP_001303310 DinQ-like type I toxin DqlB -
HTI69_RS00325 50013..50297 - 285 WP_001707309 hypothetical protein -
HTI69_RS00330 50581..50790 + 210 WP_000062603 HEAT repeat domain-containing protein -
HTI69_RS00335 50862..51536 - 675 WP_223866377 plasmid IncI1-type surface exclusion protein ExcA -
HTI69_RS00340 51586..53754 - 2169 WP_000698357 DotA/TraY family protein traY
HTI69_RS00345 53851..54435 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HTI69_RS00350 54464..55666 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
HTI69_RS00355 55633..56247 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
HTI69_RS00360 56247..59291 - 3045 WP_001024782 IncI1-type conjugal transfer protein TraU traU
HTI69_RS00365 59381..60181 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
HTI69_RS00370 60165..60353 - 189 WP_001277255 putative conjugal transfer protein TraS -
HTI69_RS00375 60417..60821 - 405 WP_000086959 IncI1-type conjugal transfer protein TraR traR
HTI69_RS00380 60872..61399 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HTI69_RS00385 61399..62103 - 705 WP_000801919 IncI1-type conjugal transfer protein TraP traP
HTI69_RS00390 62103..63392 - 1290 WP_001272003 conjugal transfer protein TraO traO
HTI69_RS00395 63395..64378 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
HTI69_RS00400 64389..65081 - 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
HTI69_RS00405 65078..65425 - 348 WP_001055900 conjugal transfer protein traL
HTI69_RS00410 65443..69210 - 3768 WP_001141527 LPD7 domain-containing protein -
HTI69_RS00415 69300..69851 - 552 WP_000014583 phospholipase D family protein -
HTI69_RS00420 69866..70156 - 291 WP_001299214 hypothetical protein traK
HTI69_RS00425 70153..71301 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HTI69_RS00430 71298..72116 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
HTI69_RS00435 72113..72571 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HTI69_RS00440 72966..73550 - 585 WP_000977522 histidine phosphatase family protein -
HTI69_RS00445 73610..74812 - 1203 WP_000976351 conjugal transfer protein TraF -
HTI69_RS00450 74897..75721 - 825 WP_001238939 conjugal transfer protein TraE traE
HTI69_RS00455 75872..77026 - 1155 WP_001139957 site-specific integrase -
HTI69_RS00460 77872..78027 - 156 WP_001302705 hypothetical protein -
HTI69_RS00465 78155..78412 + 258 WP_001302706 hypothetical protein -
HTI69_RS00580 78442..78663 + 222 Protein_93 prepilin -
HTI69_RS00475 78660..80084 - 1425 WP_001389385 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTI69_RS00480 80084..80740 - 657 WP_001193553 prepilin peptidase -
HTI69_RS00485 80725..81285 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
HTI69_RS00490 81295..81909 - 615 WP_000959785 type 4 pilus major pilin -
HTI69_RS00495 81927..83024 - 1098 WP_001208805 type II secretion system F family protein -
HTI69_RS00500 83037..84590 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
HTI69_RS00505 84601..85053 - 453 WP_001247336 type IV pilus biogenesis protein PilP -
HTI69_RS00510 85040..86335 - 1296 WP_000752774 type 4b pilus protein PilO2 -
HTI69_RS00515 86328..88010 - 1683 WP_032311054 PilN family type IVB pilus formation outer membrane protein -
HTI69_RS00520 88024..88461 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
HTI69_RS00525 88461..89528 - 1068 WP_000742600 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   3582 GenBank   NZ_KX058576
Plasmid name   pS10584 Incompatibility group   IncI1
Plasmid size   94697 bp Coordinate of oriT [Strand]   40824..40912 [-]
Host baterium   Salmonella enterica strain SJTUF10584

Cargo genes

Drug resistance gene   blaCMY-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -