Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103133
Name   oriT_pOP-I in_silico
Organism   Enterobacter cloacae subsp. cloacae strain MN201516
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KY126370 (50399..50485 [+], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_pOP-I
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTAATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2350 GenBank   WP_172689589
Name   Relaxase_HTL24_RS00220_pOP-I insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102435.55 Da        Isoelectric Point: 7.9064

>WP_172689589.1 relaxase/mobilization nuclease domain-containing protein [Enterobacter cloacae]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVKASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKAKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLVGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRDRNQSLSEYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKD
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLTRKLGEFVPPAQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYATARLRASLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDPTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGGLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIEMLIGSKQFSWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERIKPVSRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQVIHDECRMRKARIRIEHRDPLVRKLHYHIAELQRMQALITLKEAMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDSRCVVICEPGGTPMYSGVPGLQASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNTGDYDALKMDMVKVAPVLFSRSSTVAMAPEGNNEQFNTAFAKM
VAWHNINHRDDGEYQISRPDIDQVRVESEMRFAEMADQGRNPEHERNEPGSNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure



No available structure.




T4CP

ID   2079 GenBank   WP_172689587
Name   trbC_HTL24_RS00210_pOP-I insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87424.66 Da        Isoelectric Point: 6.5761

>WP_172689587.1 F-type conjugative transfer protein TrbC [Enterobacter cloacae]
MNNTPVNQALMKRNAWNSPVLELMRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRVFPSLFQYETTQESIGKGIFYMGYKRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IQNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAGLYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHKLE
GLDSEALEITKYKGQFPYMNYLDEVGAYYTERIAVQATQVRSLEFALIMMSQDQERIENQTSAANVATLM
QNAGTKVAGKIVSDDKTARTISNAAGQEARANMVSLQRQDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIESQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKASRSEPWKIIDTFQQRLANRQEAHNLMTEYDMDHSGREENLWEEALEIIRTTTMAERTI
RYITLNKAESESTEVNAEEIKLAPDAILRTLTLPQPAYVPPARYRNEPFISPKLPGSQHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-525)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 9830..44613

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTL24_RS00030 5088..5894 + 807 WP_236901763 TcpQ domain-containing protein -
HTL24_RS00035 5891..6331 + 441 WP_236901764 type IV pilus biogenesis protein PilM -
HTL24_RS00040 6346..8022 + 1677 WP_172689563 PilN family type IVB pilus formation outer membrane protein -
HTL24_RS00045 8024..9328 + 1305 WP_172689564 type 4b pilus protein PilO2 -
HTL24_RS00050 9312..9827 + 516 WP_172689565 type IV pilus biogenesis protein PilP -
HTL24_RS00055 9830..11389 + 1560 WP_172689566 ATPase, T2SS/T4P/T4SS family virB11
HTL24_RS00060 11382..12476 + 1095 WP_172689627 type II secretion system F family protein -
HTL24_RS00065 12568..13104 + 537 WP_236901766 type 4 pilus major pilin -
HTL24_RS00070 13189..13671 + 483 WP_172689628 lytic transglycosylase domain-containing protein virB1
HTL24_RS00075 13656..14330 + 675 WP_172689568 prepilin peptidase -
HTL24_RS00080 14327..15664 + 1338 WP_172689629 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTL24_RS00085 16527..17687 + 1161 WP_048210079 site-specific integrase -
HTL24_RS00090 (MN002) 17764..18183 + 420 WP_075262562 hypothetical protein -
HTL24_RS00095 18329..19165 + 837 WP_172689569 hypothetical protein traE
HTL24_RS00100 19268..20476 + 1209 WP_172689570 conjugal transfer protein TraF -
HTL24_RS00105 (MN003) 20497..21015 + 519 WP_172689571 hypothetical protein -
HTL24_RS00110 21103..21576 + 474 WP_172689572 DotD/TraH family lipoprotein -
HTL24_RS00115 21573..22379 + 807 WP_172689573 type IV secretory system conjugative DNA transfer family protein traI
HTL24_RS00120 22391..23551 + 1161 WP_172689574 plasmid transfer ATPase TraJ virB11
HTL24_RS00125 23548..23835 + 288 WP_048210072 IcmT/TraK family protein traK
HTL24_RS00130 (MN004) 23907..27455 + 3549 WP_172689575 LPD7 domain-containing protein -
HTL24_RS00135 27473..27823 + 351 WP_048210070 hypothetical protein traL
HTL24_RS00140 27829..28530 + 702 WP_172689576 DotI/IcmL/TraM family protein traM
HTL24_RS00145 28541..29524 + 984 WP_172689577 DotH/IcmK family type IV secretion protein traN
HTL24_RS00150 29524..30840 + 1317 WP_172689578 conjugal transfer protein TraO traO
HTL24_RS00155 30824..31594 + 771 WP_172689579 conjugal transfer protein TraP traP
HTL24_RS00160 31594..32118 + 525 WP_172689580 conjugal transfer protein TraQ traQ
HTL24_RS00165 32153..32557 + 405 WP_172689581 DUF6750 family protein traR
HTL24_RS00170 32742..33473 + 732 WP_236901765 conjugal transfer protein TraT traT
HTL24_RS00175 33554..36607 + 3054 WP_172689582 ATP-binding protein traU
HTL24_RS00180 37189..38391 + 1203 WP_048210062 conjugal transfer protein TraW traW
HTL24_RS00185 38388..38930 + 543 WP_053070179 conjugal transfer protein TraX -
HTL24_RS00190 38988..41150 + 2163 WP_172689583 DotA/TraY family protein traY
HTL24_RS00195 41211..41843 + 633 WP_172689584 plasmid IncI1-type surface exclusion protein ExcA -
HTL24_RS00200 42156..43364 + 1209 WP_172689585 conjugal transfer protein TrbA trbA
HTL24_RS00205 43417..44613 + 1197 WP_172689586 thioredoxin fold domain-containing protein trbB
HTL24_RS00210 44617..46917 + 2301 WP_172689587 F-type conjugative transfer protein TrbC -
HTL24_RS00215 (MN005) 46990..47259 + 270 WP_172689588 hypothetical protein -

Host bacterium

ID   3576 GenBank   NZ_KY126370
Plasmid name   pOP-I Incompatibility group   -
Plasmid size   114969 bp Coordinate of oriT [Strand]   50399..50485 [+]
Host baterium   Enterobacter cloacae subsp. cloacae strain MN201516

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -