Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103130 |
| Name | oriT_pGTC2  |
| Organism | Enterococcus faecalis strain |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KY270848 (47160..47194 [+], 35 nt) |
| oriT length | 35 nt |
| IRs (inverted repeats) | 1..8, 13..20 (GTAACATG..CATGTTAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 35 nt
>oriT_pGTC2
GTAACATGCTATCATGTTACTATGCTCGCAAAAGG
GTAACATGCTATCATGTTACTATGCTCGCAAAAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2347 | GenBank | WP_172689405 |
| Name | Relaxase_HTK87_RS00220_pGTC2 |
UniProt ID | _ |
| Length | 569 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 569 a.a. Molecular weight: 65388.16 Da Isoelectric Point: 6.8301
>WP_172689405.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFTIHTMDHLNQSKEYVQKASKTTVKKTPSGNTHYEQLFPYIMNADKTLSKQLVSGYGITDVTNA
ANEFLYTKERVAKQKGTYLMVDPETGTMVFNKESIEKGNGRGQAVLAHHLVQSFSPEDDLTPEEIHEIGR
QTVLEFTGGEYEFVIATHVDRKHIHNHIIINSTNLFTGKALPWKVVAFKNGETKDYTKELFEKVSDKISS
KHGAKIIEKSPKNSHKKYTIWQTESIYKSKIKSRLDFLLAHSSSIEDFKRKAEALELHVDFSGKWATYRL
LDEPQIKNTRSRSLSKKNPEKYNLKQIETRLKENTGSFSIEEVVDRYEEKVNVAKNDFDYQLIVEPWQIS
HKTKKGYYLTVDFGIESRGQLFIGAYKVDELEDGNYSLFVKANDFFYFMNEKNGQRNRYMTGYTLAKQLS
LYNGTVPLKKEPVMSTINELVDAINFLAEHGVTEGNQMERLEKQLVEAFETAEKSLTTLDEKILQLNQVG
KLLLANELEEYEGELPILTQLGEKEELTYEDVKSELASLKLSRKLLQEKMETTVEKINELHAIQTVAEKK
AQEEHLGKG
MVYTKHFTIHTMDHLNQSKEYVQKASKTTVKKTPSGNTHYEQLFPYIMNADKTLSKQLVSGYGITDVTNA
ANEFLYTKERVAKQKGTYLMVDPETGTMVFNKESIEKGNGRGQAVLAHHLVQSFSPEDDLTPEEIHEIGR
QTVLEFTGGEYEFVIATHVDRKHIHNHIIINSTNLFTGKALPWKVVAFKNGETKDYTKELFEKVSDKISS
KHGAKIIEKSPKNSHKKYTIWQTESIYKSKIKSRLDFLLAHSSSIEDFKRKAEALELHVDFSGKWATYRL
LDEPQIKNTRSRSLSKKNPEKYNLKQIETRLKENTGSFSIEEVVDRYEEKVNVAKNDFDYQLIVEPWQIS
HKTKKGYYLTVDFGIESRGQLFIGAYKVDELEDGNYSLFVKANDFFYFMNEKNGQRNRYMTGYTLAKQLS
LYNGTVPLKKEPVMSTINELVDAINFLAEHGVTEGNQMERLEKQLVEAFETAEKSLTTLDEKILQLNQVG
KLLLANELEEYEGELPILTQLGEKEELTYEDVKSELASLKLSRKLLQEKMETTVEKINELHAIQTVAEKK
AQEEHLGKG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2077 | GenBank | WP_172689399 |
| Name | t4cp2_HTK87_RS00185_pGTC2 |
UniProt ID | _ |
| Length | 603 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 603 a.a. Molecular weight: 69713.36 Da Isoelectric Point: 5.9893
>WP_172689399.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQIYKKNIKNYLILACLLFVLFYELGNFFWFLPGSDYEAKWFFLFSDELGLYVSTMWPTFFLTPSFPGFF
AGIFGFLLGMLGYVYDNDRGVYRTGEEHGSARFATVEEIKKFADENPANDMIFTKNARMGLFNRRLPFNY
QKNKNILVLGGPGSGKTHTFIKPNLMQRNASFIVTDPKGLLVHETGAMLKKDGYEIKVFDLVNLSNSNSF
NVFHYIKTELDVDRVLEFITEGTKKSEQQGEDFWIQAEALLIRSLIAYLWFDGKDNEYTPNLSMIADMLR
HMERKDPKVPSPVECWFEEQNKIRPNNYAYKQWTLYNDSYKAETRASVLGIASARYSVFDHEQVIDMVRE
DTMEIDKWNEEKTAVFIAIPETSSAYNFLAAIFLATAMETLKNKADRVFQGLEQLKDGRQLLHVRFLIDE
FYNIGRIPNIDKALGSFRSREMSIVMVLQAMNQLEAMYKNTWKTLVNTCDSLLFLGGDEEDTTRYLSKRA
GKQTISLRKHTINRGKNGSGSENRDSTGRDLLMPDEIGRLGNGKALLFISGQHVFKDNKFTVQDHENAHL
LADNYQDPNWYTYKRYRNEEEEILDKVRPENMIDHGVVGEDIA
MQIYKKNIKNYLILACLLFVLFYELGNFFWFLPGSDYEAKWFFLFSDELGLYVSTMWPTFFLTPSFPGFF
AGIFGFLLGMLGYVYDNDRGVYRTGEEHGSARFATVEEIKKFADENPANDMIFTKNARMGLFNRRLPFNY
QKNKNILVLGGPGSGKTHTFIKPNLMQRNASFIVTDPKGLLVHETGAMLKKDGYEIKVFDLVNLSNSNSF
NVFHYIKTELDVDRVLEFITEGTKKSEQQGEDFWIQAEALLIRSLIAYLWFDGKDNEYTPNLSMIADMLR
HMERKDPKVPSPVECWFEEQNKIRPNNYAYKQWTLYNDSYKAETRASVLGIASARYSVFDHEQVIDMVRE
DTMEIDKWNEEKTAVFIAIPETSSAYNFLAAIFLATAMETLKNKADRVFQGLEQLKDGRQLLHVRFLIDE
FYNIGRIPNIDKALGSFRSREMSIVMVLQAMNQLEAMYKNTWKTLVNTCDSLLFLGGDEEDTTRYLSKRA
GKQTISLRKHTINRGKNGSGSENRDSTGRDLLMPDEIGRLGNGKALLFISGQHVFKDNKFTVQDHENAHL
LADNYQDPNWYTYKRYRNEEEEILDKVRPENMIDHGVVGEDIA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 26725..38485
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTK87_RS00120 | 21759..22802 | + | 1044 | WP_172689386 | hypothetical protein | - |
| HTK87_RS00125 | 22914..25730 | + | 2817 | WP_172689387 | SpaA isopeptide-forming pilin-related protein | - |
| HTK87_RS00130 | 26040..26471 | + | 432 | WP_172689388 | type III secretion system protein PrgE | - |
| HTK87_RS00135 | 26488..26715 | + | 228 | WP_172689389 | type III secretion system protein PrgF | - |
| HTK87_RS00140 | 26725..27522 | + | 798 | WP_172689390 | type IV secretion system protein | prgHb |
| HTK87_RS00145 | 27530..27874 | + | 345 | WP_236918929 | PrgI family protein | prgIc |
| HTK87_RS00150 | 27828..30203 | + | 2376 | WP_172689392 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| HTK87_RS00155 | 30216..32741 | + | 2526 | WP_172689393 | phage tail tip lysozyme | prgK |
| HTK87_RS00160 | 33465..35399 | + | 1935 | WP_172689394 | group II intron reverse transcriptase/maturase | - |
| HTK87_RS00165 | 35662..36408 | + | 747 | WP_172689395 | hypothetical protein | prgL |
| HTK87_RS00170 | 36389..36667 | + | 279 | WP_172689396 | hypothetical protein | - |
| HTK87_RS00175 | 36669..37967 | + | 1299 | WP_172689397 | hypothetical protein | - |
| HTK87_RS00180 | 38000..38485 | + | 486 | WP_172689398 | DUF3801 domain-containing protein | gbs1365 |
| HTK87_RS00185 | 38485..40296 | + | 1812 | WP_172689399 | VirD4-like conjugal transfer protein, CD1115 family | - |
| HTK87_RS00190 | 40326..40787 | + | 462 | WP_172689400 | hypothetical protein | - |
| HTK87_RS00195 | 40788..41246 | + | 459 | WP_172689401 | single-stranded DNA-binding protein | - |
| HTK87_RS00200 | 41336..41539 | + | 204 | WP_172689402 | hypothetical protein | - |
Host bacterium
| ID | 3573 | GenBank | NZ_KY270848 |
| Plasmid name | pGTC2 | Incompatibility group | - |
| Plasmid size | 52325 bp | Coordinate of oriT [Strand] | 47160..47194 [+] |
| Host baterium | Enterococcus faecalis strain |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |