Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103128
• Name: oriT_pLAG
• Organism: Enterococcus faecalis strain Transconjugant T4
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_KY264168 (26867..26904 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_pLAG
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_Pre_HTL02_RS00145_pLAG

• ID: 2345
• GenBank: WP_000119405
• Name: Mob_Pre_HTL02_RS00145_pLAG
• UniProt ID: F5X340
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49660.33 Da
• Isoelectric Point: 9.9693

>WP_000119405.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: F5X340

Host bacterium

• ID: 3571
• GenBank: NZ_KY264168
• Plasmid name: pLAG
• Incompatibility group: -
• Plasmid size: 35338 bp
• Coordinate of oriT [Strand]: 26867..26904 [-]
• Host baterium: Enterococcus faecalis strain Transconjugant T4

Cargo genes

• Drug resistance gene: ant(6)-Ia, lnu(B), lsa(E), tet(L), tet(M)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -