Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103124
Name   oriT_pHS091147 in_silico
Organism   Klebsiella pneumoniae strain HS091147
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KX236178 (29426..29475 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  TGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pHS091147
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2343 GenBank   WP_047665818
Name   traI_HTI32_RS00375_pHS091147 insolico UniProt ID   A0A0S1TL23
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190754.90 Da        Isoelectric Point: 6.3385

>WP_047665818.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKDAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDAFVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTGSSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAMGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQLRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLTQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(10-284)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

AAA domain (AAA_30)

(971-1157)

DNA helicase TraI (TraI_C)

(1433-1555)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)


  Protein structure



No available structure.




T4CP

ID   2072 GenBank   WP_013023832
Name   traD_HTI32_RS00370_pHS091147 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 85974.10 Da        Isoelectric Point: 5.1149

>WP_013023832.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDYVEIGGNF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 28868..57357

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTI32_RS00165 24023..24340 + 318 WP_014343505 hypothetical protein -
HTI32_RS00170 25182..25538 + 357 WP_014343501 hypothetical protein -
HTI32_RS00175 25599..25811 + 213 WP_014343500 hypothetical protein -
HTI32_RS00180 25822..26046 + 225 WP_014343499 hypothetical protein -
HTI32_RS00185 26127..26447 + 321 WP_014343498 type II toxin-antitoxin system RelE/ParE family toxin -
HTI32_RS00190 26437..26715 + 279 WP_004152721 helix-turn-helix transcriptional regulator -
HTI32_RS00750 26719..26823 + 105 Protein_40 transcriptional regulator -
HTI32_RS00195 27652..28473 + 822 WP_172688376 DUF932 domain-containing protein -
HTI32_RS00200 28506..28835 + 330 WP_013214015 DUF5983 family protein -
HTI32_RS00205 28868..29353 - 486 WP_014343495 transglycosylase SLT domain-containing protein virB1
HTI32_RS00210 29744..30160 + 417 WP_014343494 conjugal transfer relaxosome DNA-binding protein TraM -
HTI32_RS00215 30360..31097 + 738 WP_013214018 conjugal transfer protein TrbJ -
HTI32_RS00220 31212..31418 + 207 WP_171773970 TraY domain-containing protein -
HTI32_RS00225 31480..31848 + 369 WP_004194426 type IV conjugative transfer system pilin TraA -
HTI32_RS00230 31862..32167 + 306 WP_004144424 type IV conjugative transfer system protein TraL traL
HTI32_RS00235 32187..32753 + 567 WP_004144423 type IV conjugative transfer system protein TraE traE
HTI32_RS00240 32740..33480 + 741 WP_013214019 type-F conjugative transfer system secretin TraK traK
HTI32_RS00245 33480..34904 + 1425 WP_013214020 F-type conjugal transfer pilus assembly protein TraB traB
HTI32_RS00250 34976..35560 + 585 WP_014343493 type IV conjugative transfer system lipoprotein TraV traV
HTI32_RS00255 35883..36101 + 219 WP_004195468 hypothetical protein -
HTI32_RS00260 36102..36413 + 312 WP_013214022 hypothetical protein -
HTI32_RS00265 36480..36884 + 405 WP_004197817 hypothetical protein -
HTI32_RS00270 37263..37661 + 399 WP_014343490 hypothetical protein -
HTI32_RS00275 37733..40372 + 2640 WP_172688377 type IV secretion system protein TraC virb4
HTI32_RS00280 40372..40761 + 390 WP_013214025 type-F conjugative transfer system protein TrbI -
HTI32_RS00285 40761..41396 + 636 WP_013214026 type-F conjugative transfer system protein TraW traW
HTI32_RS00290 41431..41832 + 402 WP_004194979 hypothetical protein -
HTI32_RS00295 41829..42818 + 990 WP_013214027 conjugal transfer pilus assembly protein TraU traU
HTI32_RS00300 42831..43469 + 639 WP_004193871 type-F conjugative transfer system pilin assembly protein TrbC trbC
HTI32_RS00305 43528..45483 + 1956 WP_014343489 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HTI32_RS00310 45515..45769 + 255 WP_172688378 conjugal transfer protein TrbE -
HTI32_RS00315 45747..45995 + 249 WP_013214029 hypothetical protein -
HTI32_RS00320 46008..46334 + 327 WP_172688379 hypothetical protein -
HTI32_RS00325 46355..47107 + 753 WP_004152677 type-F conjugative transfer system pilin assembly protein TraF traF
HTI32_RS00330 47118..47359 + 242 Protein_68 type-F conjugative transfer system pilin chaperone TraQ -
HTI32_RS00335 47331..47888 + 558 WP_013214031 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HTI32_RS00340 47934..48377 + 444 WP_014343488 F-type conjugal transfer protein TrbF -
HTI32_RS00345 48355..49734 + 1380 WP_014343487 conjugal transfer pilus assembly protein TraH traH
HTI32_RS00350 49734..52583 + 2850 WP_013609534 conjugal transfer mating-pair stabilization protein TraG traG
HTI32_RS00355 52586..53119 + 534 WP_014343486 conjugal transfer protein TraS -
HTI32_RS00360 53305..54036 + 732 WP_013023830 conjugal transfer complement resistance protein TraT -
HTI32_RS00365 54229..54918 + 690 WP_013023831 hypothetical protein -
HTI32_RS00370 55045..57357 + 2313 WP_013023832 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   3567 GenBank   NZ_KX236178
Plasmid name   pHS091147 Incompatibility group   IncFII
Plasmid size   121348 bp Coordinate of oriT [Strand]   29426..29475 [-]
Host baterium   Klebsiella pneumoniae strain HS091147

Cargo genes

Drug resistance gene   blaTEM-1B, dfrA25, qacE, sul1, qnrB52, blaCTX-M-14, blaKPC-2
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9