Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103123 |
| Name | oriT_pMCR_1511  |
| Organism | Klebsiella pneumoniae strain WCHKP1511 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KX377410 (25601..25703 [+], 103 nt) |
| oriT length | 103 nt |
| IRs (inverted repeats) | 64..70, 73..79 (CCACCGT..ACGGTGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 103 nt
>oriT_pMCR_1511
AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCAGCTGCGAATAAGGGAAGGTAAAGTTGGACCACCGTTTACGGTGGGCCTACTTTACATATCCTGCCCGG
AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCAGCTGCGAATAAGGGAAGGTAAAGTTGGACCACCGTTTACGGTGGGCCTACTTTACATATCCTGCCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2342 | GenBank | WP_077270386 |
| Name | Relaxase_HTI73_RS00135_pMCR_1511 |
UniProt ID | _ |
| Length | 869 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 869 a.a. Molecular weight: 96535.47 Da Isoelectric Point: 10.4435
>WP_077270386.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MKNIKKSSFSGLADYITNPQSKQERVGVIKATNCQSQDVQDAVTEVNITQRLNTRSESDKTYHLVISFRP
GEKPSEDVLNAIEESICQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPDTLTIHNPYNDYRVLAESCAY
LEKKYGLEVDNHMPTRSASENRAKDMERHSGIQSLLSWVKAECAEQLLNANSWQELHQAMRQNGLELRQK
GNGFVIAGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRPSPEAIKQRFGSAANVKRPGDRPP
PMRENRQVHLGDVPVMQINSGKRYESKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAKVKRDRLIE
NAKRSGRLKRATIKLLKGPGVNKRYLYGLASKVLGEELERANAIYKKDRQAAYDIHKRRTWADWLQFKAA
GGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGKRLDVSR
DAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKKAAREREYI
HIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDAGRERSGNG
NATGANEPGVTGRSTSADAGNGYGTSSSRRRTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPNGERGHSKP
NVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGAGGITTPAE
VKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIVVMPVSDYT
ARRLSNLKVGESVTLTPSGSVRTTKGRSR
MKNIKKSSFSGLADYITNPQSKQERVGVIKATNCQSQDVQDAVTEVNITQRLNTRSESDKTYHLVISFRP
GEKPSEDVLNAIEESICQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPDTLTIHNPYNDYRVLAESCAY
LEKKYGLEVDNHMPTRSASENRAKDMERHSGIQSLLSWVKAECAEQLLNANSWQELHQAMRQNGLELRQK
GNGFVIAGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRPSPEAIKQRFGSAANVKRPGDRPP
PMRENRQVHLGDVPVMQINSGKRYESKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAKVKRDRLIE
NAKRSGRLKRATIKLLKGPGVNKRYLYGLASKVLGEELERANAIYKKDRQAAYDIHKRRTWADWLQFKAA
GGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGKRLDVSR
DAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKKAAREREYI
HIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDAGRERSGNG
NATGANEPGVTGRSTSADAGNGYGTSSSRRRTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPNGERGHSKP
NVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGAGGITTPAE
VKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIVVMPVSDYT
ARRLSNLKVGESVTLTPSGSVRTTKGRSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2071 | GenBank | WP_032310210 |
| Name | t4cp2_HTI73_RS00125_pMCR_1511 |
UniProt ID | _ |
| Length | 648 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 648 a.a. Molecular weight: 71711.82 Da Isoelectric Point: 8.3842
>WP_032310210.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAVAANDDASNEAIAI
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAVAANDDASNEAIAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 41117..51366
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTI73_RS00225 | 36186..36383 | - | 198 | WP_048207659 | DUF905 family protein | - |
| HTI73_RS00375 | 36473..36742 | - | 270 | WP_228719037 | hypothetical protein | - |
| HTI73_RS00380 | 36844..37056 | - | 213 | WP_223307920 | hypothetical protein | - |
| HTI73_RS00235 | 37053..37301 | - | 249 | WP_071443967 | antirestriction protein ArdR | - |
| HTI73_RS00240 | 37301..37609 | - | 309 | WP_003019666 | HigA family addiction module antitoxin | - |
| HTI73_RS00245 | 37618..37917 | - | 300 | WP_071443968 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTI73_RS00250 | 39191..40069 | - | 879 | WP_048207660 | plasmid replication initiator TrfA | - |
| HTI73_RS00260 | 40459..40863 | + | 405 | WP_048207662 | transcriptional regulator | - |
| HTI73_RS00265 | 41117..42004 | + | 888 | WP_235927881 | P-type conjugative transfer ATPase TrbB | virB11 |
| HTI73_RS00270 | 42118..42501 | + | 384 | WP_003019650 | TrbC/VirB2 family protein | virB2 |
| HTI73_RS00275 | 42504..42830 | + | 327 | WP_003019648 | conjugal transfer protein TrbD | virB3 |
| HTI73_RS00280 | 42827..45370 | + | 2544 | WP_071443970 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| HTI73_RS00285 | 45367..46074 | + | 708 | WP_048207665 | VirB8/TrbF family protein | virB8 |
| HTI73_RS00290 | 46090..46980 | + | 891 | WP_003019641 | P-type conjugative transfer protein TrbG | virB9 |
| HTI73_RS00295 | 46983..47435 | + | 453 | WP_032194780 | conjugal transfer protein TrbH | - |
| HTI73_RS00300 | 47439..48833 | + | 1395 | WP_048207667 | TrbI/VirB10 family protein | virB10 |
| HTI73_RS00305 | 48846..49616 | + | 771 | WP_046960647 | P-type conjugative transfer protein TrbJ | virB5 |
| HTI73_RS00310 | 49629..49802 | + | 174 | WP_024252956 | entry exclusion lipoprotein TrbK | - |
| HTI73_RS00315 | 49807..51366 | + | 1560 | WP_048207669 | P-type conjugative transfer protein TrbL | virB6 |
| HTI73_RS00320 | 51377..51682 | + | 306 | WP_032310194 | lipoprotein | - |
| HTI73_RS00325 | 51698..52468 | + | 771 | WP_048207671 | conjugal transfer protein | - |
| HTI73_RS00330 | 52485..53171 | + | 687 | WP_053065212 | TraX family protein | - |
| HTI73_RS00335 | 53368..54222 | - | 855 | Protein_68 | Tn3 family transposase | - |
| HTI73_RS00340 | 54414..54884 | + | 471 | Protein_69 | sulfatase-like hydrolase/transferase | - |
| HTI73_RS00345 | 55002..55382 | + | 381 | WP_039026395 | diacylglycerol kinase | - |
| HTI73_RS00350 | 55457..55696 | + | 240 | WP_228719038 | hypothetical protein | - |
Host bacterium
| ID | 3566 | GenBank | NZ_KX377410 |
| Plasmid name | pMCR_1511 | Incompatibility group | IncP1 |
| Plasmid size | 57278 bp | Coordinate of oriT [Strand] | 25601..25703 [+] |
| Host baterium | Klebsiella pneumoniae strain WCHKP1511 |
Cargo genes
| Drug resistance gene | mcr-1.1, blaTEM-1B |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |