Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103117
Name   oriT_pIMP-KP1495 in_silico
Organism   Klebsiella pneumoniae strain CRE1495
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KU862632 (13783..13883 [-], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pIMP-KP1495
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2337 GenBank   WP_012561166
Name   TrwC_HTI11_RS00110_pIMP-KP1495 insolico UniProt ID   D7RU03
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB D7RU03


Auxiliary protein

ID   991 GenBank   WP_001749975
Name   WP_001749975_pIMP-KP1495 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   2065 GenBank   WP_000342688
Name   t4cp2_HTI11_RS00105_pIMP-KP1495 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 27866..38190

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTI11_RS00145 22989..23603 + 615 WP_014839983 recombinase family protein -
HTI11_RS00150 23922..24578 - 657 WP_001516695 quinolone resistance pentapeptide repeat protein QnrS1 -
HTI11_RS00155 24973..25604 + 632 Protein_30 transposase -
HTI11_RS00160 25658..26362 - 705 WP_001067855 IS6-like element IS26 family transposase -
HTI11_RS00165 26427..26606 - 180 Protein_32 helix-turn-helix domain-containing protein -
HTI11_RS00350 26873..27159 + 287 Protein_33 DUF6710 family protein -
HTI11_RS00175 27333..27866 - 534 WP_000792636 phospholipase D family protein -
HTI11_RS00180 27866..28861 - 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
HTI11_RS00185 28903..30063 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
HTI11_RS00190 30063..30947 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
HTI11_RS00195 30957..31655 - 699 WP_000646594 type IV secretion system protein virB8
HTI11_RS00200 31645..31803 - 159 WP_012561180 hypothetical protein -
HTI11_RS00205 31874..32914 - 1041 WP_001749958 type IV secretion system protein virB6
HTI11_RS00210 32930..33157 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
HTI11_RS00215 33165..33929 - 765 WP_013149461 type IV secretion system protein virB5
HTI11_RS00220 33896..36496 - 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
HTI11_RS00225 36496..36813 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
HTI11_RS00230 36863..37156 - 294 WP_001749962 hypothetical protein virB2
HTI11_RS00235 37166..37447 - 282 WP_000440698 transcriptional repressor KorA -
HTI11_RS00240 37456..38190 - 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
HTI11_RS00245 38233..38604 + 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
HTI11_RS00250 38620..38964 + 345 WP_001749964 hypothetical protein -
HTI11_RS00255 38961..39275 + 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
HTI11_RS00260 39389..39622 + 234 WP_001191790 hypothetical protein -
HTI11_RS00265 39672..40319 + 648 WP_015344958 restriction endonuclease -
HTI11_RS00270 40324..40530 + 207 WP_001749967 hypothetical protein -
HTI11_RS00275 40541..40813 - 273 Protein_54 IS1 family transposase -
HTI11_RS00280 40912..42345 + 1434 WP_001288432 DNA cytosine methyltransferase -

Host bacterium

ID   3560 GenBank   NZ_KU862632
Plasmid name   pIMP-KP1495 Incompatibility group   IncN
Plasmid size   51591 bp Coordinate of oriT [Strand]   13783..13883 [-]
Host baterium   Klebsiella pneumoniae strain CRE1495

Cargo genes

Drug resistance gene   qnrS1, blaIMP-4
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -