Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103113 |
| Name | oriT_pOXAAPSS2  |
| Organism | Klebsiella pneumoniae |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KU159086 (24934..25039 [-], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pOXAAPSS2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2334 | GenBank | WP_050868819 |
| Name | Relaxase_HTG99_RS00115_pOXAAPSS2 |
UniProt ID | A0A142BMF1 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75234.98 Da Isoelectric Point: 10.0285
>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142BMF1 |
T4CP
| ID | 2060 | GenBank | WP_011091092 |
| Name | t4cp2_HTG99_RS00420_pOXAAPSS2 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79491.81 Da Isoelectric Point: 4.8270
>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3171..22138
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTG99_RS00005 | 857..1924 | - | 1068 | WP_050868820 | plasmid replication initiator RepA | - |
| HTG99_RS00010 | 2210..2458 | - | 249 | WP_015062836 | IncL/M type plasmid replication protein RepC | - |
| HTG99_RS00015 | 2515..3168 | - | 654 | WP_011091084 | hypothetical protein | - |
| HTG99_RS00020 | 3171..5285 | - | 2115 | WP_228717364 | DotA/TraY family protein | traY |
| HTG99_RS00025 | 5343..5993 | - | 651 | WP_011091083 | hypothetical protein | - |
| HTG99_RS00030 | 5990..7198 | - | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| HTG99_RS00035 | 7195..10245 | - | 3051 | WP_181371929 | conjugal transfer protein | traU |
| HTG99_RS00040 | 10242..10736 | - | 495 | WP_011091080 | hypothetical protein | - |
| HTG99_RS00045 | 10782..11171 | - | 390 | WP_011091078 | DUF6750 family protein | traR |
| HTG99_RS00050 | 11188..11718 | - | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| HTG99_RS00055 | 11742..12446 | - | 705 | WP_011091076 | conjugal transfer protein TraP | traP |
| HTG99_RS00060 | 12458..13807 | - | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| HTG99_RS00065 | 13819..14970 | - | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| HTG99_RS00070 | 14979..15761 | - | 783 | WP_011091073 | DotI/IcmL family type IV secretion protein | traM |
| HTG99_RS00075 | 15760..16170 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| HTG99_RS00080 | 16157..16354 | + | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| HTG99_RS00085 | 16355..16909 | - | 555 | WP_011154467 | hypothetical protein | traL |
| HTG99_RS00445 | 16833..19910 | - | 3078 | WP_220181703 | LPD7 domain-containing protein | - |
| HTG99_RS00095 | 19935..20195 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| HTG99_RS00100 | 20185..21348 | - | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| HTG99_RS00105 | 21359..22138 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| HTG99_RS00110 | 22135..22635 | - | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| HTG99_RS00115 | 22649..24628 | - | 1980 | WP_050868819 | TraI/MobA(P) family conjugative relaxase | - |
| HTG99_RS00450 | 24615..25193 | - | 579 | WP_223811519 | plasmid mobilization protein MobA | - |
| HTG99_RS00125 | 25208..25573 | + | 366 | WP_011091067 | hypothetical protein | - |
| HTG99_RS00130 | 26074..26610 | - | 537 | WP_011091066 | hypothetical protein | - |
| HTG99_RS00135 | 26743..27054 | - | 312 | WP_011091065 | hypothetical protein | - |
Host bacterium
| ID | 3556 | GenBank | NZ_KU159086 |
| Plasmid name | pOXAAPSS2 | Incompatibility group | IncL/M |
| Plasmid size | 66284 bp | Coordinate of oriT [Strand] | 24934..25039 [-] |
| Host baterium | Klebsiella pneumoniae |
Cargo genes
| Drug resistance gene | blaOXA-48, blaTEM-1B |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |