Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103112
• Name: oriT_pWBG637
• Organism: Staphylococcus aureus strain WBG1024
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_KX086582 (23658..23846 [+], 189 nt)
• oriT length: 189 nt
• IRs (inverted repeats): 149..155, 162..168 (CTATCAT..ATGATAG) 132..138, 142..148 (GTCTGGC..GCCAGAC) 33..39, 44..50 (TGTCACG..CGTGACA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 189 nt

>oriT_pWBG637
TGTCACAAAAATAAGACAAAATCTACATGTAGTGTCACGAAACCGTGACAAACACAATATATAGTGTCACAAAAATGTGACACTAGGTGTTTTTATGATATCACTATGAAATGTCCTAAAACCCTTGGAATGTCTGGCTTTGCCAGACCTATCATTGTACGATGATAGCAAATTCCCCTTATGCTCTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobL_HTI01_RS00165_pWBG637

• ID: 2333
• GenBank: WP_223248946
• Name: MobL_HTI01_RS00165_pWBG637
• UniProt ID: _
• Length: 383 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 383 a.a.
• Molecular weight: 45925.28 Da
• Isoelectric Point: 9.8763

>WP_223248946.1 MobP2 family relaxase [Staphylococcus aureus]
MMPKIVTVSQFEQPNEFYEYSNYVNYMKRGEAQNNKDDFKYDVYAHYMFDEEKSQNMFNEHDNFMSKKAI
NKIKSDFSHAQKNKGMMWKDVISFDNSSLESVGIYDSKTHTLDEQKIKEATRKMMKRFEKKEGLEDNLVW
SGAIHYNTDNIHVHVAAVEKVIKRTRGKRKPATLKHMKSTFANELFDIKGERQKINEFIRERIVKGIRDE
SEPCKDKAMKKQIKKVHKLLEDIPKNQWNYNNNILKYIRPEIDKITDIYIQNYHSKDFEVFKNDLKRQNN
LYKETYGENSNYGSYEKTKMKDLYARSGNTILKNIKELDQDLMKVKQQFTHSKLNVTITKLKIGKCLNQA
LYRTKYALRSDYDKEKNIQQYYQEFDKQSYRER

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (2-351)

Protein structure

No available structure.

T4CP

t4cp2_HTI01_RS00115_pWBG637

• ID: 2059
• GenBank: WP_172687394
• Name: t4cp2_HTI01_RS00115_pWBG637
• UniProt ID: _
• Length: 923 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 923 a.a.
• Molecular weight: 106160.18 Da
• Isoelectric Point: 8.0720

>WP_172687394.1 VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus aureus]
MRFGSKDKHGYRRKKLSFSQNKHLLAHPCILVSILLLIMLLATAIANFLINILMTVFFGNHDIFTNMKQL
NTKYTDYIFNFRSDAVLLYIPIWVLVFIFFGKKVYQFRQRFKSLNNNEKASGRFATRKEITQQYKAIPQR
EHAFEGEGGLPVAMFGVYDFVTKTKQYLEQRKTAIREINGITDLTADEKLELRKDKLGKMNKEFATTFFS
KREFTFIDESPTNNLIVGTSRSGKGEIVVLSMIENYSRSSEQPSLIVNDMKGELFSASYKTLENRGYQVE
IFNLDQPMESTMSFNPLQQVIDEYMKGDLGEAQEMTKQITYTLTNNEGVEDNEWNLMAGALINAMILALC
EETLPKNPEKVTLYSVSNMLQTLSTKRFYKEIAIGNKVQLIEVSALDEYMERFPSHSPAKTQYATVQAAP
DKMRSSIIGTALKALQPFTTDTIAKLTSHTSFDINKLGFPSIIDGYATKDSTFELGVQVLRDGENGIEYE
EVEGIQIGVNEYGYWQYPFKTVLKVGDRIETIEKDGNNQVITSYFYVDHIDDKGQVTFNQKGELDNSDIT
IRKFNSFPKPIAVFMVVPDYNNANHGIASILVNQIVFEISKNSQLYTENQSTYRRVIFHLDEAGNMPPIP
NLSQKVNVSLSRGLRFNFFVQAFSQFKDKYGDAYDAIMDACQNKVYIMATQEQTLKDFSAMIGSQQITVH
SRSGEAGAIKSSITENQEERPLLRPDELARLQEGETVVVRNLKRQDKKRNKVMSYPIFNDGKYAMKYRYQ
YLSDLMDTSQSIIHFRPMLKARCEHRHLQLEATLVDWDKRIEEMQEGIDGQKEENKNINDINQYKKSNEE
GNVAVRVKRLETLKDKVGDAVFDKITRRFERYLRTYAEQGKNVNTITQKKLEELVLADSEVKEEEKTKRI
EMIKKLIKEAKTT

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (615-732)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (209-458)

Protein structure

No available structure.

Host bacterium

• ID: 3555
• GenBank: NZ_KX086582
• Plasmid name: pWBG637
• Incompatibility group: -
• Plasmid size: 38025 bp
• Coordinate of oriT [Strand]: 23658..23846 [+]
• Host baterium: Staphylococcus aureus strain WBG1024

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21