Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103109 |
| Name | oriT_pKPSH11  |
| Organism | Klebsiella pneumoniae strain I11 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KT896504 (146843..146892 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKPSH11
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2330 | GenBank | WP_015065542 |
| Name | traI_HUK39_RS00715_pKPSH11 |
UniProt ID | K7R3U7 |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190318.03 Da Isoelectric Point: 6.2890
>WP_015065542.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2057 | GenBank | WP_015065541 |
| Name | traD_HUK39_RS00720_pKPSH11 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 86025.00 Da Isoelectric Point: 5.0577
>WP_015065541.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 117394..147450
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUK39_RS00720 | 117394..119706 | - | 2313 | WP_015065541 | type IV conjugative transfer system coupling protein TraD | virb4 |
| HUK39_RS00725 | 119835..120524 | - | 690 | WP_072198073 | hypothetical protein | - |
| HUK39_RS00730 | 120717..121448 | - | 732 | WP_016831056 | conjugal transfer complement resistance protein TraT | - |
| HUK39_RS01145 | 121699..122043 | - | 345 | WP_159376579 | hypothetical protein | - |
| HUK39_RS00740 | 122324..125146 | - | 2823 | WP_016831055 | conjugal transfer mating-pair stabilization protein TraG | traG |
| HUK39_RS00745 | 125146..126525 | - | 1380 | WP_072159542 | conjugal transfer pilus assembly protein TraH | traH |
| HUK39_RS00750 | 126503..126946 | - | 444 | WP_016831053 | F-type conjugal transfer protein TrbF | - |
| HUK39_RS00755 | 126992..127549 | - | 558 | WP_004152678 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| HUK39_RS00760 | 127521..127760 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| HUK39_RS00765 | 127771..128523 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| HUK39_RS00770 | 128544..128870 | - | 327 | WP_016831051 | hypothetical protein | - |
| HUK39_RS00775 | 128883..129131 | - | 249 | WP_004152675 | hypothetical protein | - |
| HUK39_RS00780 | 129109..129363 | - | 255 | WP_015065558 | conjugal transfer protein TrbE | - |
| HUK39_RS00785 | 129395..131350 | - | 1956 | WP_023313940 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| HUK39_RS00790 | 131409..132056 | - | 648 | WP_039109927 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| HUK39_RS00795 | 132332..133321 | - | 990 | WP_015632509 | conjugal transfer pilus assembly protein TraU | traU |
| HUK39_RS00800 | 133318..133719 | - | 402 | WP_016831047 | hypothetical protein | - |
| HUK39_RS00805 | 133754..134389 | - | 636 | WP_016831046 | type-F conjugative transfer system protein TraW | traW |
| HUK39_RS00810 | 134389..134778 | - | 390 | WP_004167468 | type-F conjugative transfer system protein TrbI | - |
| HUK39_RS00815 | 134778..137417 | - | 2640 | WP_016831045 | type IV secretion system protein TraC | virb4 |
| HUK39_RS00820 | 137489..137887 | - | 399 | WP_023158008 | hypothetical protein | - |
| HUK39_RS00825 | 137895..138188 | - | 294 | WP_020325117 | hypothetical protein | - |
| HUK39_RS00830 | 138328..138732 | - | 405 | WP_016831042 | hypothetical protein | - |
| HUK39_RS00835 | 138799..139110 | - | 312 | WP_016831041 | hypothetical protein | - |
| HUK39_RS00840 | 139111..139329 | - | 219 | WP_014386199 | hypothetical protein | - |
| HUK39_RS00845 | 139353..139642 | - | 290 | Protein_169 | hypothetical protein | - |
| HUK39_RS00850 | 139647..140057 | - | 411 | WP_032409654 | hypothetical protein | - |
| HUK39_RS00855 | 140189..140773 | - | 585 | WP_014386196 | type IV conjugative transfer system lipoprotein TraV | traV |
| HUK39_RS00860 | 140796..141392 | - | 597 | Protein_172 | conjugal transfer pilus-stabilizing protein TraP | - |
| HUK39_RS00865 | 141385..142809 | - | 1425 | WP_016831036 | F-type conjugal transfer pilus assembly protein TraB | traB |
| HUK39_RS00870 | 142809..143549 | - | 741 | WP_032408905 | type-F conjugative transfer system secretin TraK | traK |
| HUK39_RS00875 | 143536..144102 | - | 567 | WP_016831035 | type IV conjugative transfer system protein TraE | traE |
| HUK39_RS00880 | 144122..144427 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| HUK39_RS00885 | 144441..144809 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| HUK39_RS00890 | 144871..145077 | - | 207 | WP_171773970 | TraY domain-containing protein | - |
| HUK39_RS00895 | 145239..145958 | - | 720 | WP_016831034 | conjugal transfer protein TrbJ | - |
| HUK39_RS00900 | 146158..146574 | - | 417 | WP_072145360 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| HUK39_RS00905 | 146965..147450 | + | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| HUK39_RS00910 | 147483..147812 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| HUK39_RS00915 | 147845..148666 | - | 822 | WP_004182076 | DUF932 domain-containing protein | - |
| HUK39_RS00920 | 149751..150314 | - | 564 | WP_004182054 | methyltransferase | - |
| HUK39_RS00925 | 150362..151717 | - | 1356 | WP_004182052 | DUF3560 domain-containing protein | - |
| HUK39_RS00930 | 151769..151999 | - | 231 | WP_001568051 | hypothetical protein | - |
| HUK39_RS00935 | 152091..152318 | - | 228 | WP_004182050 | hypothetical protein | - |
Host bacterium
| ID | 3552 | GenBank | NZ_KT896504 |
| Plasmid name | pKPSH11 | Incompatibility group | IncFII |
| Plasmid size | 186474 bp | Coordinate of oriT [Strand] | 146843..146892 [+] |
| Host baterium | Klebsiella pneumoniae strain I11 |
Cargo genes
| Drug resistance gene | sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, blaCTX-M-15, aac(3)-IIa, blaOXA-1, aac(6')-Ib-cr, tet(A), qnrB1, dfrA14 |
| Virulence gene | - |
| Metal resistance gene | silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |