Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103099 |
| Name | oriT_kpn240-BG  |
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain 240-BG |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KT852335 (64269..64350 [-], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_kpn240-BG
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2321 | GenBank | WP_015632446 |
| Name | Relaxase_HTG52_RS00310_kpn240-BG |
UniProt ID | R4WBX2 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74631.63 Da Isoelectric Point: 9.3060
>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R4WBX2 |
T4CP
| ID | 2047 | GenBank | WP_015632442 |
| Name | trbC_HTG52_RS00275_kpn240-BG |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82688.74 Da Isoelectric Point: 5.8919
>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34567..56408
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTG52_RS00140 | 30120..30506 | - | 387 | WP_015632417 | acyl-CoA thioesterase | - |
| HTG52_RS00145 | 30506..30991 | - | 486 | WP_015632418 | hypothetical protein | - |
| HTG52_RS00150 | 30958..31536 | - | 579 | WP_015632419 | hypothetical protein | - |
| HTG52_RS00155 | 31804..32337 | + | 534 | WP_015632420 | transcription termination/antitermination NusG family protein | - |
| HTG52_RS00160 | 32355..32558 | + | 204 | WP_032495743 | hypothetical protein | - |
| HTG52_RS00165 | 32592..32903 | + | 312 | WP_015632421 | hypothetical protein | - |
| HTG52_RS00170 | 33601..34080 | + | 480 | WP_015632423 | lytic transglycosylase domain-containing protein | - |
| HTG52_RS00175 | 34103..34570 | + | 468 | WP_015632424 | DotD/TraH family lipoprotein | - |
| HTG52_RS00180 | 34567..35385 | + | 819 | WP_015632425 | type IV secretory system conjugative DNA transfer family protein | traI |
| HTG52_RS00185 | 35363..36550 | + | 1188 | WP_032495744 | plasmid transfer ATPase TraJ | virB11 |
| HTG52_RS00190 | 36547..36813 | + | 267 | WP_032441926 | IcmT/TraK family protein | traK |
| HTG52_RS00195 | 36831..40154 | + | 3324 | WP_032495745 | LPD7 domain-containing protein | - |
| HTG52_RS00200 | 40188..40541 | + | 354 | WP_015632427 | hypothetical protein | traL |
| HTG52_RS00205 | 40577..41281 | + | 705 | WP_015632428 | DotI/IcmL/TraM family protein | traM |
| HTG52_RS00210 | 41292..42395 | + | 1104 | WP_015632429 | DotH/IcmK family type IV secretion protein | traN |
| HTG52_RS00215 | 42399..43739 | + | 1341 | WP_015632430 | conjugal transfer protein TraO | traO |
| HTG52_RS00220 | 43745..44464 | + | 720 | WP_015632431 | conjugal transfer protein TraP | traP |
| HTG52_RS00225 | 44474..45007 | + | 534 | WP_015632432 | conjugal transfer protein TraQ | traQ |
| HTG52_RS00230 | 45061..45462 | + | 402 | WP_015632433 | DUF6750 family protein | traR |
| HTG52_RS00235 | 45506..46069 | + | 564 | WP_032495746 | hypothetical protein | traT |
| HTG52_RS00240 | 46088..49126 | + | 3039 | WP_335904770 | ATP-binding protein | - |
| HTG52_RS00245 | 49173..50333 | + | 1161 | WP_032495747 | conjugal transfer protein TraW | traW |
| HTG52_RS00250 | 50330..50911 | + | 582 | WP_015632437 | conjugal transfer protein TraX | - |
| HTG52_RS00255 | 50953..53097 | + | 2145 | WP_015632438 | DotA/TraY family protein | traY |
| HTG52_RS00260 | 53163..53786 | + | 624 | WP_015632439 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HTG52_RS00265 | 53888..55306 | + | 1419 | WP_015632440 | hypothetical protein | trbA |
| HTG52_RS00270 | 55254..56408 | + | 1155 | WP_015632441 | thioredoxin fold domain-containing protein | trbB |
| HTG52_RS00275 | 56410..58602 | + | 2193 | WP_015632442 | F-type conjugative transfer protein TrbC | - |
| HTG52_RS00280 | 58648..58953 | + | 306 | WP_015632443 | phospholipase D-like domain-containing protein | - |
| HTG52_RS00285 | 58976..60565 | - | 1590 | WP_015632444 | IS66 family transposase | - |
| HTG52_RS00290 | 60595..60944 | - | 350 | Protein_57 | IS66 family insertion sequence element accessory protein TnpB | - |
| HTG52_RS00295 | 60941..61303 | - | 363 | WP_032495750 | transposase | - |
Host bacterium
| ID | 3542 | GenBank | NZ_KT852335 |
| Plasmid name | kpn240-BG | Incompatibility group | IncFII |
| Plasmid size | 76980 bp | Coordinate of oriT [Strand] | 64269..64350 [-] |
| Host baterium | Klebsiella pneumoniae subsp. pneumoniae strain 240-BG |
Cargo genes
| Drug resistance gene | blaNDM-1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |