Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103096 |
| Name | oriT_pOXAAPSS1  |
| Organism | Klebsiella pneumoniae |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KU159085 (29838..29943 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pOXAAPSS1
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2318 | GenBank | WP_004187323 |
| Name | Relaxase_HTG51_RS00270_pOXAAPSS1 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 2044 | GenBank | WP_228719393 |
| Name | t4cp2_HTG51_RS00400_pOXAAPSS1 |
UniProt ID | _ |
| Length | 560 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 560 a.a. Molecular weight: 64000.30 Da Isoelectric Point: 6.4338
>WP_228719393.1 conjugal transfer protein TrbC [Klebsiella pneumoniae]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGRG
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGRG
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 32739..60632
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTG51_RS00250 | 27823..28134 | + | 312 | WP_004187333 | hypothetical protein | - |
| HTG51_RS00255 | 28267..28803 | + | 537 | WP_004187332 | hypothetical protein | - |
| HTG51_RS00260 | 29305..29700 | - | 396 | WP_019725163 | hypothetical protein | - |
| HTG51_RS00450 | 29699..30262 | + | 564 | WP_020277892 | plasmid mobilization protein MobA | - |
| HTG51_RS00270 | 30249..32228 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| HTG51_RS00275 | 32242..32742 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| HTG51_RS00280 | 32739..33518 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| HTG51_RS00285 | 33529..34692 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| HTG51_RS00290 | 34682..34942 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| HTG51_RS00295 | 34967..36505 | + | 1539 | WP_181372791 | toprim domain-containing protein | - |
| HTG51_RS00300 | 36505..38178 | + | 1674 | WP_227601284 | LPD7 domain-containing protein | - |
| HTG51_RS00305 | 38144..38656 | + | 513 | WP_011091071 | hypothetical protein | traL |
| HTG51_RS00310 | 38657..38854 | - | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| HTG51_RS00315 | 38841..39251 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| HTG51_RS00320 | 39250..40032 | + | 783 | WP_015058941 | DotI/IcmL family type IV secretion protein | traM |
| HTG51_RS00455 | 40041..40181 | + | 141 | WP_228719390 | hypothetical protein | - |
| HTG51_RS00325 | 40221..41192 | + | 972 | WP_228719391 | DotH/IcmK family type IV secretion protein | traN |
| HTG51_RS00330 | 41204..42553 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| HTG51_RS00335 | 42565..43269 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| HTG51_RS00340 | 43293..43823 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| HTG51_RS00345 | 43840..44229 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| HTG51_RS00350 | 44275..44769 | + | 495 | WP_004187480 | hypothetical protein | - |
| HTG51_RS00355 | 44766..47816 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| HTG51_RS00360 | 47813..49021 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| HTG51_RS00365 | 49018..49614 | + | 597 | WP_015060003 | hypothetical protein | - |
| HTG51_RS00370 | 49607..50587 | + | 981 | WP_228719392 | DotA/TraY family protein | traY |
| HTG51_RS00375 | 50621..51318 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| HTG51_RS00380 | 51365..52579 | + | 1215 | WP_077273780 | DotA/TraY family protein | traY |
| HTG51_RS00385 | 52581..53234 | + | 654 | WP_015060005 | hypothetical protein | - |
| HTG51_RS00390 | 53303..53545 | + | 243 | WP_023893611 | IncL/M type plasmid replication protein RepC | - |
| HTG51_RS00395 | 53829..54896 | + | 1068 | WP_019725043 | plasmid replication initiator RepA | - |
| HTG51_RS00465 | 56119..56214 | + | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| HTG51_RS00460 | 56265..56591 | - | 327 | WP_064424685 | hypothetical protein | - |
| HTG51_RS00400 | 56669..58351 | - | 1683 | WP_228719393 | conjugal transfer protein TrbC | - |
| HTG51_RS00405 | 58364..59314 | - | 951 | WP_004206886 | DsbC family protein | trbB |
| HTG51_RS00410 | 59325..60632 | - | 1308 | WP_015059988 | hypothetical protein | trbA |
| HTG51_RS00415 | 60632..60988 | - | 357 | WP_015586032 | lytic transglycosylase domain-containing protein | - |
| HTG51_RS00420 | 61132..61527 | - | 396 | WP_015059989 | DUF1496 domain-containing protein | - |
| HTG51_RS00425 | 61595..62029 | + | 435 | Protein_87 | CPBP family intramembrane glutamate endopeptidase | - |
| HTG51_RS00430 | 62044..63252 | - | 1209 | WP_001206290 | IS4-like element IS10A family transposase | - |
Host bacterium
| ID | 3539 | GenBank | NZ_KU159085 |
| Plasmid name | pOXAAPSS1 | Incompatibility group | IncL/M |
| Plasmid size | 63359 bp | Coordinate of oriT [Strand] | 29838..29943 [+] |
| Host baterium | Klebsiella pneumoniae |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA8 |