Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103095 |
| Name | oriT_Kpn235-BG  |
| Organism | Klebsiella pneumoniae strain Kpn235 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KT852336 (63962..64043 [-], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_Kpn235-BG
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2317 | GenBank | WP_015632446 |
| Name | Relaxase_HTG69_RS00300_Kpn235-BG |
UniProt ID | R4WBX2 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74631.63 Da Isoelectric Point: 9.3060
>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R4WBX2 |
T4CP
| ID | 2043 | GenBank | WP_015632442 |
| Name | trbC_HTG69_RS00265_Kpn235-BG |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82688.74 Da Isoelectric Point: 5.8919
>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34260..56101
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTG69_RS00130 | 29813..30199 | - | 387 | WP_015632417 | acyl-CoA thioesterase | - |
| HTG69_RS00135 | 30199..30684 | - | 486 | WP_015632418 | hypothetical protein | - |
| HTG69_RS00140 | 30651..31229 | - | 579 | WP_015632419 | hypothetical protein | - |
| HTG69_RS00145 | 31563..32030 | + | 468 | WP_032495742 | transcription termination/antitermination NusG family protein | - |
| HTG69_RS00150 | 32048..32251 | + | 204 | WP_032495743 | hypothetical protein | - |
| HTG69_RS00155 | 32285..32596 | + | 312 | WP_015632421 | hypothetical protein | - |
| HTG69_RS00160 | 33294..33773 | + | 480 | WP_015632423 | lytic transglycosylase domain-containing protein | - |
| HTG69_RS00165 | 33796..34263 | + | 468 | WP_015632424 | DotD/TraH family lipoprotein | - |
| HTG69_RS00170 | 34260..35078 | + | 819 | WP_015632425 | type IV secretory system conjugative DNA transfer family protein | traI |
| HTG69_RS00175 | 35056..36243 | + | 1188 | WP_032495744 | plasmid transfer ATPase TraJ | virB11 |
| HTG69_RS00180 | 36240..36506 | + | 267 | WP_032441926 | IcmT/TraK family protein | traK |
| HTG69_RS00185 | 36524..39847 | + | 3324 | WP_032495745 | LPD7 domain-containing protein | - |
| HTG69_RS00190 | 39881..40234 | + | 354 | WP_015632427 | hypothetical protein | traL |
| HTG69_RS00195 | 40270..40974 | + | 705 | WP_015632428 | DotI/IcmL/TraM family protein | traM |
| HTG69_RS00200 | 40985..42088 | + | 1104 | WP_015632429 | DotH/IcmK family type IV secretion protein | traN |
| HTG69_RS00205 | 42092..43432 | + | 1341 | WP_015632430 | conjugal transfer protein TraO | traO |
| HTG69_RS00210 | 43438..44157 | + | 720 | WP_015632431 | conjugal transfer protein TraP | traP |
| HTG69_RS00215 | 44167..44700 | + | 534 | WP_015632432 | conjugal transfer protein TraQ | traQ |
| HTG69_RS00220 | 44754..45155 | + | 402 | WP_015632433 | DUF6750 family protein | traR |
| HTG69_RS00225 | 45199..45762 | + | 564 | WP_032495746 | hypothetical protein | traT |
| HTG69_RS00230 | 45781..48819 | + | 3039 | WP_335904770 | ATP-binding protein | - |
| HTG69_RS00235 | 48866..50026 | + | 1161 | WP_032495747 | conjugal transfer protein TraW | traW |
| HTG69_RS00240 | 50023..50604 | + | 582 | WP_015632437 | conjugal transfer protein TraX | - |
| HTG69_RS00245 | 50646..52790 | + | 2145 | WP_015632438 | DotA/TraY family protein | traY |
| HTG69_RS00250 | 52856..53479 | + | 624 | WP_015632439 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HTG69_RS00255 | 53581..54999 | + | 1419 | WP_015632440 | hypothetical protein | trbA |
| HTG69_RS00260 | 54947..56101 | + | 1155 | WP_015632441 | thioredoxin fold domain-containing protein | trbB |
| HTG69_RS00265 | 56103..58295 | + | 2193 | WP_015632442 | F-type conjugative transfer protein TrbC | - |
| HTG69_RS00270 | 58341..58646 | + | 306 | WP_015632443 | phospholipase D-like domain-containing protein | - |
| HTG69_RS00275 | 58669..60258 | - | 1590 | WP_015632444 | IS66 family transposase | - |
| HTG69_RS00280 | 60288..60637 | - | 350 | Protein_55 | IS66 family insertion sequence element accessory protein TnpB | - |
| HTG69_RS00285 | 60634..60996 | - | 363 | WP_032495750 | transposase | - |
Host bacterium
| ID | 3538 | GenBank | NZ_KT852336 |
| Plasmid name | Kpn235-BG | Incompatibility group | IncFII |
| Plasmid size | 76365 bp | Coordinate of oriT [Strand] | 63962..64043 [-] |
| Host baterium | Klebsiella pneumoniae strain Kpn235 |
Cargo genes
| Drug resistance gene | blaNDM-1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |