Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103084
Name   oriT_pEC15I_1 in_silico
Organism   Escherichia coli
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KU932029 (60923..61011 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pEC15I_1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2307 GenBank   WP_001360283
Name   nikB_HTI61_RS00315_pEC15I_1 insolico UniProt ID   A0A637A499
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104011.40 Da        Isoelectric Point: 7.3426

>WP_001360283.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A637A499


Auxiliary protein

ID   969 GenBank   WP_001283947
Name   WP_001283947_pEC15I_1 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2029 GenBank   WP_024255997
Name   trbC_HTI61_RS00310_pEC15I_1 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86940.16 Da        Isoelectric Point: 7.0662

>WP_024255997.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLKCADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 17244..55545

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTI61_RS00085 12306..13373 + 1068 WP_001492935 type IV pilus biogenesis lipoprotein PilL -
HTI61_RS00090 13373..13810 + 438 WP_000539807 type IV pilus biogenesis protein PilM -
HTI61_RS00095 13824..15506 + 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HTI61_RS00100 15499..16794 + 1296 WP_001774172 type 4b pilus protein PilO2 -
HTI61_RS00105 16781..17233 + 453 WP_001247870 type IV pilus biogenesis protein PilP -
HTI61_RS00110 17244..18797 + 1554 WP_001492932 ATPase, T2SS/T4P/T4SS family virB11
HTI61_RS00115 18810..19907 + 1098 WP_001492931 type II secretion system F family protein -
HTI61_RS00120 19925..20539 + 615 WP_001542527 type 4 pilus major pilin -
HTI61_RS00125 20549..21109 + 561 WP_001774171 lytic transglycosylase domain-containing protein virB1
HTI61_RS00130 21094..21750 + 657 WP_001193554 prepilin peptidase -
HTI61_RS00135 21750..23042 + 1293 WP_001417545 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTI61_RS00140 23039..23239 - 201 WP_236922357 hypothetical protein -
HTI61_RS00145 23977..24234 + 258 WP_001302706 hypothetical protein -
HTI61_RS00150 24339..24494 + 156 WP_001302705 hypothetical protein -
HTI61_RS00510 24491..24809 - 319 Protein_31 shufflon protein C -
HTI61_RS00155 24808..25962 + 1155 WP_001139958 site-specific integrase -
HTI61_RS00160 26113..26937 + 825 WP_001238935 conjugal transfer protein TraE traE
HTI61_RS00165 27022..28224 + 1203 WP_000976353 conjugal transfer protein TraF -
HTI61_RS00170 28284..28868 + 585 WP_000977523 histidine phosphatase family protein -
HTI61_RS00175 29263..29721 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HTI61_RS00180 29718..30536 + 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
HTI61_RS00185 30533..31681 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HTI61_RS00190 31678..31968 + 291 WP_001299214 hypothetical protein traK
HTI61_RS00195 31983..32534 + 552 WP_000014584 phospholipase D family protein -
HTI61_RS00200 32625..36392 + 3768 WP_181365823 LPD7 domain-containing protein -
HTI61_RS00205 36410..36757 + 348 WP_001055900 conjugal transfer protein traL
HTI61_RS00210 36754..37446 + 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
HTI61_RS00215 37457..38440 + 984 WP_001191879 IncI1-type conjugal transfer protein TraN traN
HTI61_RS00220 38443..39732 + 1290 WP_001774189 conjugal transfer protein TraO traO
HTI61_RS00225 39732..40436 + 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HTI61_RS00230 40436..40963 + 528 WP_001055569 conjugal transfer protein TraQ traQ
HTI61_RS00235 41014..41418 + 405 WP_000086959 IncI1-type conjugal transfer protein TraR traR
HTI61_RS00240 41482..41670 + 189 WP_001277255 putative conjugal transfer protein TraS -
HTI61_RS00245 41654..42454 + 801 WP_181365824 IncI1-type conjugal transfer protein TraT traT
HTI61_RS00250 42544..45588 + 3045 WP_001024786 IncI1-type conjugal transfer protein TraU traU
HTI61_RS00255 45588..46202 + 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
HTI61_RS00260 46169..47371 + 1203 WP_001385654 IncI1-type conjugal transfer protein TraW traW
HTI61_RS00265 47400..47984 + 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HTI61_RS00270 48081..50249 + 2169 WP_001774191 DotA/TraY family protein traY
HTI61_RS00275 50299..50973 + 675 WP_223866377 plasmid IncI1-type surface exclusion protein ExcA -
HTI61_RS00280 51045..51254 - 210 WP_000062603 HEAT repeat domain-containing protein -
HTI61_RS00285 51538..51822 + 285 WP_181365821 hypothetical protein -
HTI61_RS00520 51887..51982 - 96 WP_001303310 DinQ-like type I toxin DqlB -
HTI61_RS00290 52481..52732 + 252 WP_001291964 hypothetical protein -
HTI61_RS00295 52804..52956 - 153 WP_001387489 Hok/Gef family protein -
HTI61_RS00300 53248..54456 + 1209 WP_024255995 IncI1-type conjugal transfer protein TrbA trbA
HTI61_RS00305 54475..55545 + 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
HTI61_RS00310 55538..57829 + 2292 WP_024255997 F-type conjugative transfer protein TrbC -

Host bacterium

ID   3527 GenBank   NZ_KU932029
Plasmid name   pEC15I_1 Incompatibility group   IncI1
Plasmid size   87811 bp Coordinate of oriT [Strand]   60923..61011 [+]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -