Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103081 |
| Name | oriT_pEC3II_2  |
| Organism | Escherichia coli |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KU932023 (3086..3138 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pEC3II_2
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2024 | GenBank | WP_181365836 |
| Name | t4cp2_HTH51_RS00160_pEC3II_2 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73294.84 Da Isoelectric Point: 8.9020
>WP_181365836.1 type IV secretory system conjugative DNA transfer family protein [Escherichia coli]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRCVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRCVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 20104..43678
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTH51_RS00095 | 15505..16158 | - | 654 | WP_000572551 | hypothetical protein | - |
| HTH51_RS00100 | 16170..17294 | - | 1125 | WP_000486716 | site-specific integrase | - |
| HTH51_RS00105 | 17432..17587 | + | 156 | WP_001358489 | hypothetical protein | - |
| HTH51_RS00110 | 18208..19452 | - | 1245 | WP_000750517 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTH51_RS00115 | 19465..20100 | - | 636 | WP_000934978 | A24 family peptidase | - |
| HTH51_RS00120 | 20104..20532 | - | 429 | WP_001326801 | lytic transglycosylase domain-containing protein | virB1 |
| HTH51_RS00125 | 20652..21209 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| HTH51_RS00130 | 21254..22363 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTH51_RS00135 | 22354..23907 | - | 1554 | WP_000466228 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTH51_RS00140 | 23932..24426 | - | 495 | WP_000912555 | type IV pilus biogenesis protein PilP | - |
| HTH51_RS00145 | 24410..25732 | - | 1323 | WP_010895889 | type 4b pilus protein PilO2 | - |
| HTH51_RS00150 | 25771..27414 | - | 1644 | WP_001035589 | PilN family type IVB pilus formation outer membrane protein | - |
| HTH51_RS00155 | 27407..27925 | - | 519 | WP_010895890 | sigma 54-interacting transcriptional regulator | virb4 |
| HTH51_RS00160 | 27972..29930 | - | 1959 | WP_181365836 | type IV secretory system conjugative DNA transfer family protein | - |
| HTH51_RS00165 | 29946..31001 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTH51_RS00170 | 31132..32271 | - | 1140 | WP_000790641 | TrbI/VirB10 family protein | virB10 |
| HTH51_RS00175 | 32261..32962 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTH51_RS00180 | 33028..33762 | - | 735 | WP_000432283 | type IV secretion system protein | virB8 |
| HTH51_RS00185 | 33928..36285 | - | 2358 | WP_010895892 | VirB4 family type IV secretion system protein | virb4 |
| HTH51_RS00190 | 36291..36611 | - | 321 | WP_010895893 | VirB3 family type IV secretion system protein | virB3 |
| HTH51_RS00195 | 36682..36972 | - | 291 | WP_000865478 | TrbC/VirB2 family protein | virB2 |
| HTH51_RS00200 | 36972..37556 | - | 585 | WP_001177114 | lytic transglycosylase domain-containing protein | virB1 |
| HTH51_RS00205 | 37577..37975 | - | 399 | WP_001153669 | hypothetical protein | - |
| HTH51_RS00210 | 38094..38531 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HTH51_RS00215 | 38537..39772 | - | 1236 | WP_000733391 | toxin co-regulated pilus biosynthesis Q family protein | - |
| HTH51_RS00220 | 39775..40062 | - | 288 | WP_029785630 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTH51_RS00225 | 40234..40869 | - | 636 | WP_000835769 | hypothetical protein | - |
| HTH51_RS00230 | 40917..41726 | + | 810 | WP_001005153 | DUF5710 domain-containing protein | - |
| HTH51_RS00235 | 41779..42033 | - | 255 | WP_000609210 | EexN family lipoprotein | - |
| HTH51_RS00240 | 42036..42677 | - | 642 | WP_001434037 | type IV secretion system protein | - |
| HTH51_RS00245 | 42683..43678 | - | 996 | WP_000276068 | type IV secretion system protein | virB6 |
| HTH51_RS00250 | 43682..43939 | - | 258 | WP_000739144 | hypothetical protein | - |
| HTH51_RS00255 | 43936..44238 | - | 303 | WP_000189499 | hypothetical protein | - |
| HTH51_RS00400 | 44219..44890 | - | 672 | WP_236926545 | hypothetical protein | - |
| HTH51_RS00270 | 45041..45703 | - | 663 | WP_001243159 | hypothetical protein | - |
| HTH51_RS00275 | 45714..45884 | - | 171 | WP_000550721 | hypothetical protein | - |
| HTH51_RS00280 | 45888..46331 | - | 444 | WP_000498521 | NfeD family protein | - |
| HTH51_RS00285 | 46393..47346 | - | 954 | WP_072097371 | SPFH domain-containing protein | - |
| HTH51_RS00290 | 47373..47549 | - | 177 | WP_000753050 | hypothetical protein | - |
| HTH51_RS00295 | 47542..47757 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| HTH51_RS00300 | 47750..48160 | - | 411 | WP_181365837 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 3524 | GenBank | NZ_KU932023 |
| Plasmid name | pEC3II_2 | Incompatibility group | IncI2 |
| Plasmid size | 59192 bp | Coordinate of oriT [Strand] | 3086..3138 [-] |
| Host baterium | Escherichia coli |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |