Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103077
Name   oriT_pFAM22871_2 in_silico
Organism   Escherichia coli strain FAM22871
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KU355874 (42708..42796 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pFAM22871_2
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2303 GenBank   WP_031942444
Name   nikB_HTH15_RS00290_pFAM22871_2 insolico UniProt ID   A0A075UES7
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104017.50 Da        Isoelectric Point: 7.2816

>WP_031942444.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSHMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLAERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESCYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALVDKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A075UES7


Auxiliary protein

ID   962 GenBank   WP_001283947
Name   WP_001283947_pFAM22871_2 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2019 GenBank   WP_012783931
Name   trbC_HTH15_RS00295_pFAM22871_2 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86911.02 Da        Isoelectric Point: 6.7713

>WP_012783931.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILTAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 48174..87675

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTH15_RS00295 45890..48181 - 2292 WP_012783931 F-type conjugative transfer protein TrbC -
HTH15_RS00300 48174..49244 - 1071 WP_031942445 IncI1-type conjugal transfer protein TrbB trbB
HTH15_RS00305 49263..50471 - 1209 WP_001703845 IncI1-type conjugal transfer protein TrbA trbA
HTH15_RS00310 50777..51556 - 780 WP_275542888 protein FinQ -
HTH15_RS00315 52183..52335 + 153 WP_001331364 Hok/Gef family protein -
HTH15_RS00320 52407..52658 - 252 WP_001291964 hypothetical protein -
HTH15_RS00585 53159..53254 + 96 WP_000609148 DinQ-like type I toxin DqlB -
HTH15_RS00330 53319..53495 - 177 WP_001054898 hypothetical protein -
HTH15_RS00335 53678..53887 - 210 WP_001140543 hemolysin expression modulator Hha -
HTH15_RS00340 53985..54599 - 615 WP_000578648 plasmid IncI1-type surface exclusion protein ExcA -
HTH15_RS00345 54675..56843 - 2169 WP_031942447 IncI1-type conjugal transfer membrane protein TraY traY
HTH15_RS00350 56940..57524 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HTH15_RS00355 57553..58755 - 1203 WP_031942448 IncI1-type conjugal transfer protein TraW traW
HTH15_RS00360 58722..59336 - 615 WP_031942449 IncI1-type conjugal transfer protein TraV traV
HTH15_RS00365 59336..62380 - 3045 WP_001024789 IncI1-type conjugal transfer protein TraU traU
HTH15_RS00370 62470..63270 - 801 WP_001164783 IncI1-type conjugal transfer protein TraT traT
HTH15_RS00375 63254..63442 - 189 WP_001277253 putative conjugal transfer protein TraS -
HTH15_RS00380 63506..63910 - 405 WP_000086965 IncI1-type conjugal transfer protein TraR traR
HTH15_RS00385 63961..64488 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HTH15_RS00390 64488..65192 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HTH15_RS00395 65192..66481 - 1290 WP_001271994 conjugal transfer protein TraO traO
HTH15_RS00400 66484..67467 - 984 WP_001191879 IncI1-type conjugal transfer protein TraN traN
HTH15_RS00405 67478..68170 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
HTH15_RS00410 68167..68514 - 348 WP_001055900 conjugal transfer protein traL
HTH15_RS00415 68532..72296 - 3765 WP_001141540 LPD7 domain-containing protein -
HTH15_RS00420 72386..72937 - 552 WP_000014586 phospholipase D family protein -
HTH15_RS00425 72952..73242 - 291 WP_001314267 hypothetical protein traK
HTH15_RS00430 73239..74387 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HTH15_RS00435 74384..75202 - 819 WP_031942450 IncI1-type conjugal transfer lipoprotein TraI traI
HTH15_RS00440 75199..75657 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HTH15_RS00445 76052..76636 - 585 WP_000977522 histidine phosphatase family protein -
HTH15_RS00450 76696..77898 - 1203 WP_000976351 conjugal transfer protein TraF -
HTH15_RS00455 77983..78807 - 825 WP_001238939 conjugal transfer protein TraE traE
HTH15_RS00460 78957..80111 - 1155 WP_001139957 site-specific integrase -
HTH15_RS00465 80110..80427 + 318 WP_001558195 hypothetical protein -
HTH15_RS00470 80424..80579 - 156 WP_001302705 hypothetical protein -
HTH15_RS00475 81240..81497 + 258 WP_001302706 hypothetical protein -
HTH15_RS00480 81632..81880 + 249 WP_001349157 hypothetical protein -
HTH15_RS00485 81877..83169 - 1293 WP_001417545 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTH15_RS00490 83169..83825 - 657 WP_001193553 prepilin peptidase -
HTH15_RS00495 83810..84370 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
HTH15_RS00500 84380..84994 - 615 WP_000959785 type 4 pilus major pilin -
HTH15_RS00505 85012..86109 - 1098 WP_001208805 type II secretion system F family protein -
HTH15_RS00510 86122..87675 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
HTH15_RS00515 87686..88138 - 453 WP_001247336 type IV pilus biogenesis protein PilP -
HTH15_RS00520 88125..89420 - 1296 WP_021560540 type 4b pilus protein PilO2 -
HTH15_RS00525 89413..91095 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HTH15_RS00530 91109..91546 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
HTH15_RS00535 91546..92613 - 1068 WP_031942452 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   3520 GenBank   NZ_KU355874
Plasmid name   pFAM22871_2 Incompatibility group   IncI1
Plasmid size   97819 bp Coordinate of oriT [Strand]   42708..42796 [-]
Host baterium   Escherichia coli strain FAM22871

Cargo genes

Drug resistance gene   blaCTX-M-15, aac(3)-IId, blaTEM-1B
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2