Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103073 |
| Name | oriT_pmcr1_IncI2  |
| Organism | Escherichia coli strain SZ02 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KU761326 (15838..15890 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pmcr1_IncI2
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2014 | GenBank | WP_072652803 |
| Name | t4cp2_HTH10_RS00265_pmcr1_IncI2 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73374.92 Da Isoelectric Point: 9.3476
>WP_072652803.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVIY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVIY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 35604..59627
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTH10_RS00200 | 31093..32217 | - | 1125 | WP_000486716 | site-specific integrase | - |
| HTH10_RS00425 | 32280..32501 | + | 222 | Protein_40 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTH10_RS00205 | 32896..33156 | + | 261 | WP_029237379 | hypothetical protein | - |
| HTH10_RS00210 | 33514..33669 | + | 156 | WP_001358489 | hypothetical protein | - |
| HTH10_RS00215 | 33666..34952 | - | 1287 | WP_052993113 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTH10_RS00220 | 34965..35600 | - | 636 | WP_000934977 | A24 family peptidase | - |
| HTH10_RS00225 | 35604..36086 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTH10_RS00230 | 36152..36709 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| HTH10_RS00235 | 36754..37863 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTH10_RS00240 | 37854..39392 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTH10_RS00245 | 39417..39911 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTH10_RS00250 | 39895..41217 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTH10_RS00255 | 41256..42899 | - | 1644 | WP_063084537 | PilN family type IVB pilus formation outer membrane protein | - |
| HTH10_RS00260 | 42892..43428 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HTH10_RS00265 | 43475..45433 | - | 1959 | WP_072652803 | type IV secretory system conjugative DNA transfer family protein | - |
| HTH10_RS00270 | 45449..46504 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTH10_RS00275 | 46523..47662 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| HTH10_RS00280 | 47652..48353 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTH10_RS00285 | 48419..49153 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTH10_RS00290 | 49319..51676 | - | 2358 | WP_000548955 | VirB4 family type IV secretion system protein | virb4 |
| HTH10_RS00295 | 51682..52002 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTH10_RS00300 | 52073..52363 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HTH10_RS00305 | 52363..52947 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| HTH10_RS00310 | 52968..53366 | - | 399 | WP_001153665 | hypothetical protein | - |
| HTH10_RS00315 | 53485..53922 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HTH10_RS00320 | 53928..55163 | - | 1236 | WP_072652802 | TcpQ domain-containing protein | - |
| HTH10_RS00325 | 55166..55465 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTH10_RS00330 | 55533..55814 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTH10_RS00335 | 55804..56055 | - | 252 | WP_000121741 | hypothetical protein | - |
| HTH10_RS00340 | 56155..56790 | - | 636 | WP_015059536 | hypothetical protein | - |
| HTH10_RS00345 | 56838..57629 | + | 792 | WP_226102801 | DUF5710 domain-containing protein | - |
| HTH10_RS00350 | 57737..57982 | - | 246 | WP_063080371 | EexN family lipoprotein | - |
| HTH10_RS00355 | 57982..58623 | - | 642 | WP_062897948 | type IV secretion system protein | - |
| HTH10_RS00360 | 58629..59627 | - | 999 | WP_063084539 | type IV secretion system protein | virB6 |
| HTH10_RS00365 | 59631..59888 | - | 258 | WP_000739144 | hypothetical protein | - |
| HTH10_RS00370 | 59885..60187 | - | 303 | WP_001360345 | hypothetical protein | - |
| HTH10_RS00375 | 60203..60454 | - | 252 | WP_236904241 | hypothetical protein | - |
| HTH10_RS00380 | 60451..60633 | - | 183 | WP_022540746 | hypothetical protein | - |
| HTH10_RS00385 | 60602..61297 | - | 696 | WP_021503155 | yhbB protein | - |
| HTH10_RS00390 | 61308..61478 | - | 171 | WP_000550721 | hypothetical protein | - |
| HTH10_RS00395 | 61482..61925 | - | 444 | WP_000964333 | NfeD family protein | - |
| HTH10_RS00400 | 61928..62182 | - | 255 | WP_024181945 | hypothetical protein | - |
| HTH10_RS00405 | 62305..63258 | - | 954 | WP_072652804 | SPFH domain-containing protein | - |
| HTH10_RS00410 | 63285..63461 | - | 177 | WP_045146385 | hypothetical protein | - |
| HTH10_RS00415 | 63454..63669 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| HTH10_RS00420 | 63662..64168 | - | 507 | WP_001326595 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 3516 | GenBank | NZ_KU761326 |
| Plasmid name | pmcr1_IncI2 | Incompatibility group | IncI2 |
| Plasmid size | 64964 bp | Coordinate of oriT [Strand] | 15838..15890 [-] |
| Host baterium | Escherichia coli strain SZ02 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |