Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103072
Name   oriT_pBK33689 in_silico
Organism   Escherichia coli strain BK33689
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KU295133 (74656..74705 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  GGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pBK33689
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2300 GenBank   WP_004153015
Name   traI_HTG75_RS00615_pBK33689 insolico UniProt ID   A0A8A5TN24
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190492.51 Da        Isoelectric Point: 6.3661

>WP_004153015.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGMFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRHNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGASGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQKSVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

DNA helicase TraI (TraI_C)

(1433-1555)

TrwC relaxase (TrwC)

(10-284)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

AAA domain (AAA_30)

(971-1157)


  Protein structure



No available structure.




T4CP

ID   2013 GenBank   WP_004152620
Name   traD_HTG75_RS00610_pBK33689 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 85871.84 Da        Isoelectric Point: 5.0101

>WP_004152620.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 74098..103095

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTG75_RS00400 69199..69549 + 351 WP_004153414 hypothetical protein -
HTG75_RS00405 70179..70532 + 354 WP_004152748 hypothetical protein -
HTG75_RS00410 70589..70936 + 348 WP_004152749 hypothetical protein -
HTG75_RS00415 71031..71177 + 147 WP_004152750 hypothetical protein -
HTG75_RS00420 71228..72061 + 834 WP_004152751 N-6 DNA methylase -
HTG75_RS00425 72882..73703 + 822 WP_004152492 DUF932 domain-containing protein -
HTG75_RS00430 73736..74065 + 330 WP_011977736 DUF5983 family protein -
HTG75_RS00435 74098..74583 - 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
HTG75_RS00440 75005..75403 + 399 WP_004152493 conjugal transfer relaxosome DNA-binding protein TraM -
HTG75_RS00445 75577..76263 + 687 WP_004152494 transcriptional regulator TraJ family protein -
HTG75_RS00450 76342..76728 + 387 WP_004152495 TraY domain-containing protein -
HTG75_RS00455 76782..77150 + 369 WP_004152496 type IV conjugative transfer system pilin TraA -
HTG75_RS00460 77164..77469 + 306 WP_004144424 type IV conjugative transfer system protein TraL traL
HTG75_RS00465 77489..78055 + 567 WP_004144423 type IV conjugative transfer system protein TraE traE
HTG75_RS00470 78042..78782 + 741 WP_004152497 type-F conjugative transfer system secretin TraK traK
HTG75_RS00475 78782..80206 + 1425 WP_004155033 F-type conjugal transfer pilus assembly protein TraB traB
HTG75_RS00480 80320..80904 + 585 WP_004161368 type IV conjugative transfer system lipoprotein TraV traV
HTG75_RS00485 81036..81446 + 411 WP_004152499 hypothetical protein -
HTG75_RS00490 81552..81770 + 219 WP_004152501 hypothetical protein -
HTG75_RS00495 81771..82082 + 312 WP_004152502 hypothetical protein -
HTG75_RS00500 82149..82553 + 405 WP_004152503 hypothetical protein -
HTG75_RS00505 82596..82985 + 390 WP_004153076 hypothetical protein -
HTG75_RS00510 82993..83391 + 399 WP_011977783 hypothetical protein -
HTG75_RS00515 83463..86102 + 2640 WP_004152505 type IV secretion system protein TraC virb4
HTG75_RS00520 86102..86491 + 390 WP_004152506 type-F conjugative transfer system protein TrbI -
HTG75_RS00525 86491..87117 + 627 WP_004152507 type-F conjugative transfer system protein TraW traW
HTG75_RS00530 87159..87548 + 390 WP_004152508 hypothetical protein -
HTG75_RS00535 87545..88534 + 990 WP_011977785 conjugal transfer pilus assembly protein TraU traU
HTG75_RS00540 88547..89185 + 639 WP_004152672 type-F conjugative transfer system pilin assembly protein TrbC trbC
HTG75_RS00545 89244..91199 + 1956 WP_004152673 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HTG75_RS00550 91231..91485 + 255 WP_004152674 conjugal transfer protein TrbE -
HTG75_RS00555 91463..91711 + 249 WP_004152675 hypothetical protein -
HTG75_RS00560 91724..92050 + 327 WP_004152676 hypothetical protein -
HTG75_RS00565 92071..92823 + 753 WP_004152677 type-F conjugative transfer system pilin assembly protein TraF traF
HTG75_RS00570 92834..93073 + 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
HTG75_RS00575 93045..93602 + 558 WP_004152678 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HTG75_RS00580 93648..94091 + 444 WP_004152679 F-type conjugal transfer protein TrbF -
HTG75_RS00585 94069..95448 + 1380 WP_004165169 conjugal transfer pilus assembly protein TraH traH
HTG75_RS00590 95448..98297 + 2850 WP_004152624 conjugal transfer mating-pair stabilization protein TraG traG
HTG75_RS00595 98310..98858 + 549 WP_004152623 conjugal transfer entry exclusion protein TraS -
HTG75_RS00600 99042..99773 + 732 WP_004152622 conjugal transfer complement resistance protein TraT -
HTG75_RS00605 99965..100654 + 690 WP_004198206 hypothetical protein -
HTG75_RS00610 100783..103095 + 2313 WP_004152620 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   3515 GenBank   NZ_KU295133
Plasmid name   pBK33689 Incompatibility group   IncFIB
Plasmid size   115706 bp Coordinate of oriT [Strand]   74656..74705 [-]
Host baterium   Escherichia coli strain BK33689

Cargo genes

Drug resistance gene   blaKPC-2, blaOXA-9, blaTEM-1A
Virulence gene   -
Metal resistance gene   merE, merD, merA
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9