Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103068 |
| Name | oriT_pBK34397  |
| Organism | Escherichia coli strain BK34397 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KU295132 (63484..63532 [-], 49 nt) |
| oriT length | 49 nt |
| IRs (inverted repeats) | 6..13, 16..23 (GCAAAATT..AATTTTGC) |
| Location of nic site | 32..33 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT_pBK34397
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2297 | GenBank | WP_064291677 |
| Name | traI_HTG50_RS00590_pBK34397 |
UniProt ID | A0A6I4Y321 |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190676.77 Da Isoelectric Point: 6.3386
>WP_064291677.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKDAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTGSSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQLRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLTQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKDAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTGSSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQLRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLTQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2010 | GenBank | WP_072101984 |
| Name | traD_HTG50_RS00585_pBK34397 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85967.95 Da Isoelectric Point: 5.0577
>WP_072101984.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIS
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPASGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLTDEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIS
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPASGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLTDEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 62926..91399
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTG50_RS00375 | 57951..58301 | + | 351 | WP_023287104 | hypothetical protein | - |
| HTG50_RS00380 | 58933..59289 | + | 357 | WP_019706019 | hypothetical protein | - |
| HTG50_RS00385 | 59350..59562 | + | 213 | WP_019706020 | hypothetical protein | - |
| HTG50_RS00390 | 59573..59797 | + | 225 | WP_014343499 | hypothetical protein | - |
| HTG50_RS00395 | 59878..60198 | + | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTG50_RS00400 | 60188..60466 | + | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
| HTG50_RS00405 | 60467..60880 | + | 414 | WP_069985713 | helix-turn-helix domain-containing protein | - |
| HTG50_RS00410 | 61710..62531 | + | 822 | WP_023287138 | DUF932 domain-containing protein | - |
| HTG50_RS00415 | 62564..62893 | + | 330 | WP_071994324 | DUF5983 family protein | - |
| HTG50_RS00420 | 62926..63411 | - | 486 | WP_032440470 | transglycosylase SLT domain-containing protein | virB1 |
| HTG50_RS00425 | 63843..64235 | + | 393 | WP_004194114 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| HTG50_RS00430 | 64465..65166 | + | 702 | WP_004194113 | hypothetical protein | - |
| HTG50_RS00435 | 65252..65452 | + | 201 | WP_004194116 | TraY domain-containing protein | - |
| HTG50_RS00440 | 65521..65889 | + | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| HTG50_RS00445 | 65903..66208 | + | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| HTG50_RS00450 | 66228..66794 | + | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| HTG50_RS00455 | 66781..67521 | + | 741 | WP_013023821 | type-F conjugative transfer system secretin TraK | traK |
| HTG50_RS00460 | 67521..68945 | + | 1425 | WP_004194260 | F-type conjugal transfer pilus assembly protein TraB | traB |
| HTG50_RS00465 | 69018..69602 | + | 585 | WP_032440547 | type IV conjugative transfer system lipoprotein TraV | traV |
| HTG50_RS00470 | 69925..70143 | + | 219 | WP_004195468 | hypothetical protein | - |
| HTG50_RS00475 | 70144..70455 | + | 312 | WP_029497417 | hypothetical protein | - |
| HTG50_RS00480 | 70522..70926 | + | 405 | WP_004197817 | hypothetical protein | - |
| HTG50_RS00485 | 71305..71703 | + | 399 | WP_023179972 | hypothetical protein | - |
| HTG50_RS00490 | 71775..74414 | + | 2640 | WP_013214024 | type IV secretion system protein TraC | virb4 |
| HTG50_RS00495 | 74414..74803 | + | 390 | WP_004167468 | type-F conjugative transfer system protein TrbI | - |
| HTG50_RS00500 | 74803..75438 | + | 636 | WP_029497419 | type-F conjugative transfer system protein TraW | traW |
| HTG50_RS00505 | 75473..75874 | + | 402 | WP_004194979 | hypothetical protein | - |
| HTG50_RS00510 | 75871..76860 | + | 990 | WP_029497420 | conjugal transfer pilus assembly protein TraU | traU |
| HTG50_RS00515 | 76873..77511 | + | 639 | WP_069985712 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| HTG50_RS00520 | 77570..79525 | + | 1956 | WP_030003482 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| HTG50_RS00525 | 79557..79811 | + | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| HTG50_RS00530 | 79789..80037 | + | 249 | WP_004152675 | hypothetical protein | - |
| HTG50_RS00535 | 80050..80376 | + | 327 | WP_004152676 | hypothetical protein | - |
| HTG50_RS00540 | 80397..81149 | + | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| HTG50_RS00545 | 81160..81399 | + | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| HTG50_RS00550 | 81371..81928 | + | 558 | WP_013214031 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| HTG50_RS00555 | 81974..82417 | + | 444 | WP_004194305 | F-type conjugal transfer protein TrbF | - |
| HTG50_RS00560 | 82395..83774 | + | 1380 | WP_014343487 | conjugal transfer pilus assembly protein TraH | traH |
| HTG50_RS00565 | 83774..86623 | + | 2850 | WP_013609534 | conjugal transfer mating-pair stabilization protein TraG | traG |
| HTG50_RS00570 | 86626..87159 | + | 534 | WP_014343486 | conjugal transfer protein TraS | - |
| HTG50_RS00575 | 87345..88076 | + | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| HTG50_RS00580 | 88269..88958 | + | 690 | WP_072101983 | hypothetical protein | - |
| HTG50_RS00585 | 89087..91399 | + | 2313 | WP_072101984 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 3511 | GenBank | NZ_KU295132 |
| Plasmid name | pBK34397 | Incompatibility group | - |
| Plasmid size | 101915 bp | Coordinate of oriT [Strand] | 63484..63532 [-] |
| Host baterium | Escherichia coli strain BK34397 |
Cargo genes
| Drug resistance gene | blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |