Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103067 |
| Name | oriT_pBK28009  |
| Organism | Escherichia coli strain BK28009 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KU295131 (94592..94692 [+], 101 nt) |
| oriT length | 101 nt |
| IRs (inverted repeats) | 80..85, 91..96 (AAAAAA..TTTTTT) 20..26, 38..44 (TAAATCA..TGATTTA) |
| Location of nic site | 62..63 |
| Conserved sequence flanking the nic site |
GGTGTATAGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 101 nt
>oriT_pBK28009
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2296 | GenBank | WP_012561166 |
| Name | TrwC_HTG85_RS00540_pBK28009 |
UniProt ID | D7RU03 |
| Length | 1078 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1078 a.a. Molecular weight: 120141.30 Da Isoelectric Point: 6.5229
>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | D7RU03 |
Auxiliary protein
| ID | 955 | GenBank | WP_001749975 |
| Name | WP_001749975_pBK28009 |
UniProt ID | A0A5C2CVS6 |
| Length | 138 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 138 a.a. Molecular weight: 15332.60 Da Isoelectric Point: 5.9670
>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5C2CVS6 |
T4CP
| ID | 2007 | GenBank | WP_060568120 |
| Name | t4cp2_HTG85_RS00240_pBK28009 |
UniProt ID | _ |
| Length | 916 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 916 a.a. Molecular weight: 105022.43 Da Isoelectric Point: 7.4077
>WP_060568120.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacteriaceae]
MAILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYWLLLLLIPAFIELRRLAKEDIHFFDLKLLAF
KTRGNRAATRHFGTKALVANQYDAIDVTEFIKHMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEI
GGTIFECEDEHYLATLDMQLNNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGK
PFYQRHLFFTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARPLGTYEENGH
VFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTDGQSYFRGLEIKDYSTETITGL
YDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLKSAEDDAISQQEDLKVARDMLQSGIISFGKYHQT
LIVSAPTSEQVIRDTNTLADTLKDLGFIMTLSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHN
FHPHKRDGNAWGEAIAVVPTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQ
KYRRPESFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTKRNRNFLKKLMR
MICTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVLHEAPTKDARMNGLRIRLQQWAQGGE
FGWVFDNDVDTFDIDNIDNFGIDGTEFLDDKDVRSPLTFYLLYRVTSLLDGRRLVIFMDEFWKWLQDPEF
SKFALDMLKVIRKLNGIFFPATQSPEEMCKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDI
VTTLDPYARQMVIMKSPLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKDE
MLRRAV
MAILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYWLLLLLIPAFIELRRLAKEDIHFFDLKLLAF
KTRGNRAATRHFGTKALVANQYDAIDVTEFIKHMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEI
GGTIFECEDEHYLATLDMQLNNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGK
PFYQRHLFFTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARPLGTYEENGH
VFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTDGQSYFRGLEIKDYSTETITGL
YDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLKSAEDDAISQQEDLKVARDMLQSGIISFGKYHQT
LIVSAPTSEQVIRDTNTLADTLKDLGFIMTLSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHN
FHPHKRDGNAWGEAIAVVPTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQ
KYRRPESFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTKRNRNFLKKLMR
MICTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVLHEAPTKDARMNGLRIRLQQWAQGGE
FGWVFDNDVDTFDIDNIDNFGIDGTEFLDDKDVRSPLTFYLLYRVTSLLDGRRLVIFMDEFWKWLQDPEF
SKFALDMLKVIRKLNGIFFPATQSPEEMCKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDI
VTTLDPYARQMVIMKSPLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKDE
MLRRAV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 2008 | GenBank | WP_001776117 |
| Name | t4cp2_HTG85_RS00315_pBK28009 |
UniProt ID | _ |
| Length | 635 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 635 a.a. Molecular weight: 70357.81 Da Isoelectric Point: 6.2530
>WP_001776117.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacteriaceae]
MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFSAKHGGQEKPTFLQRILILLSMICFVF
GSIAPVAVWFLQRIYGREDIASPPLLTWTMALLFTVAGFILHVVLRRLISPEFDKVKRGMVKKTSMERDR
RTDVRRVKEILPVTTEYDPMDYIDLTKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILL
YQSILADEGVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAGFSLA
EKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGKTEELAMLNSINAPGGF
ALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRDRVAGNIRPIAIFKDEVKYHLSKPALEGL
GAARDKGVHILMAHQSIADLRDCPADLNGDAVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDES
RKVTTTAVLTEKVDNERMLRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLP
PVAVATVAEVKPVLDLDFDKTVPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPAPNDEIH
SLLDL
MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFSAKHGGQEKPTFLQRILILLSMICFVF
GSIAPVAVWFLQRIYGREDIASPPLLTWTMALLFTVAGFILHVVLRRLISPEFDKVKRGMVKKTSMERDR
RTDVRRVKEILPVTTEYDPMDYIDLTKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILL
YQSILADEGVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAGFSLA
EKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGKTEELAMLNSINAPGGF
ALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRDRVAGNIRPIAIFKDEVKYHLSKPALEGL
GAARDKGVHILMAHQSIADLRDCPADLNGDAVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDES
RKVTTTAVLTEKVDNERMLRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLP
PVAVATVAEVKPVLDLDFDKTVPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPAPNDEIH
SLLDL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 2009 | GenBank | WP_000342688 |
| Name | t4cp2_HTG85_RS00545_pBK28009 |
UniProt ID | _ |
| Length | 509 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 509 a.a. Molecular weight: 57762.87 Da Isoelectric Point: 9.6551
>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 13780..47472
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTG85_RS00060 | 8794..9120 | + | 327 | WP_003100858 | hypothetical protein | - |
| HTG85_RS00065 | 9117..9617 | + | 501 | WP_003100856 | hypothetical protein | - |
| HTG85_RS00070 | 9614..9985 | + | 372 | WP_003100853 | hypothetical protein | - |
| HTG85_RS00075 | 9979..10536 | + | 558 | WP_003100847 | recombinase family protein | - |
| HTG85_RS00080 | 10615..11514 | + | 900 | Protein_15 | IS110-like element IS4321 family transposase | - |
| HTG85_RS00085 | 11571..12275 | - | 705 | WP_001067855 | IS6-like element IS26 family transposase | - |
| HTG85_RS00090 | 12340..12519 | - | 180 | Protein_17 | helix-turn-helix domain-containing protein | - |
| HTG85_RS00650 | 12786..13073 | + | 288 | Protein_18 | DUF6710 family protein | - |
| HTG85_RS00100 | 13247..13780 | - | 534 | WP_000792636 | phospholipase D family protein | - |
| HTG85_RS00105 | 13780..14775 | - | 996 | WP_000128596 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTG85_RS00110 | 14817..15977 | - | 1161 | WP_000101710 | type IV secretion system protein VirB10 | virB10 |
| HTG85_RS00115 | 15977..16861 | - | 885 | WP_000735066 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| HTG85_RS00120 | 16872..17570 | - | 699 | WP_000646594 | type IV secretion system protein | virB8 |
| HTG85_RS00655 | 17560..17718 | - | 159 | WP_012561180 | hypothetical protein | - |
| HTG85_RS00125 | 17789..18829 | - | 1041 | WP_001749958 | type IV secretion system protein | virB6 |
| HTG85_RS00130 | 18845..19072 | - | 228 | WP_001749959 | IncN-type entry exclusion lipoprotein EexN | - |
| HTG85_RS00135 | 19080..19844 | - | 765 | WP_013149461 | type IV secretion system protein | virB5 |
| HTG85_RS00140 | 19811..22411 | - | 2601 | WP_001749961 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| HTG85_RS00145 | 22411..22728 | - | 318 | WP_000496058 | VirB3 family type IV secretion system protein | virB3 |
| HTG85_RS00150 | 22778..23071 | - | 294 | WP_001749962 | hypothetical protein | virB2 |
| HTG85_RS00155 | 23081..23362 | - | 282 | WP_000440698 | transcriptional repressor KorA | - |
| HTG85_RS00160 | 23371..24105 | - | 735 | WP_001749963 | lytic transglycosylase domain-containing protein | virB1 |
| HTG85_RS00165 | 24148..24519 | + | 372 | WP_011867773 | H-NS family nucleoid-associated regulatory protein | - |
| HTG85_RS00170 | 24535..24879 | + | 345 | WP_087920731 | hypothetical protein | - |
| HTG85_RS00175 | 24876..25190 | + | 315 | WP_001749965 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTG85_RS00180 | 25226..25537 | + | 312 | WP_001452736 | hypothetical protein | - |
| HTG85_RS00185 | 25587..26234 | + | 648 | WP_012561184 | restriction endonuclease | - |
| HTG85_RS00190 | 26239..26445 | + | 207 | WP_001749967 | hypothetical protein | - |
| HTG85_RS00195 | 26456..26728 | - | 273 | Protein_39 | IS1 family transposase | - |
| HTG85_RS00200 | 26827..28260 | + | 1434 | WP_001288432 | DNA cytosine methyltransferase | - |
| HTG85_RS00205 | 28294..29508 | - | 1215 | WP_004098817 | type II site-specific deoxyribonuclease | - |
| HTG85_RS00210 | 29564..29629 | + | 66 | Protein_42 | EAL domain-containing protein | - |
| HTG85_RS00215 | 29769..30533 | - | 765 | WP_001389365 | IS6-like element IS6100 family transposase | - |
| HTG85_RS00220 | 30651..30719 | - | 69 | Protein_44 | EAL domain-containing protein | - |
| HTG85_RS00230 | 31034..31738 | - | 705 | WP_001067855 | IS6-like element IS26 family transposase | - |
| HTG85_RS00235 | 31729..31983 | + | 255 | WP_087920708 | hypothetical protein | virB2 |
| HTG85_RS00240 | 31993..34743 | + | 2751 | WP_060568120 | VirB3 family type IV secretion system protein | virb4 |
| HTG85_RS00245 | 34762..35478 | + | 717 | WP_000770034 | type IV secretion system protein | - |
| HTG85_RS00250 | 35490..35774 | + | 285 | WP_001776107 | EexN family lipoprotein | - |
| HTG85_RS00255 | 35792..36799 | + | 1008 | WP_001776108 | type IV secretion system protein | virB6 |
| HTG85_RS00260 | 36876..37016 | + | 141 | WP_000873539 | hypothetical protein | - |
| HTG85_RS00265 | 37009..37695 | + | 687 | WP_001293055 | type IV secretion system protein | virB8 |
| HTG85_RS00270 | 37688..38581 | + | 894 | WP_039267231 | P-type conjugative transfer protein VirB9 | virB9 |
| HTG85_RS00275 | 38578..39774 | + | 1197 | WP_039267233 | VirB10/TraB/TrbI family type IV secretion system protein | - |
| HTG85_RS00280 | 39778..40827 | + | 1050 | WP_023338069 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTG85_RS00285 | 40814..41215 | + | 402 | WP_001776111 | cag pathogenicity island Cag12 family protein | - |
| HTG85_RS00290 | 41306..41623 | + | 318 | WP_000683352 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTG85_RS00295 | 41650..41985 | + | 336 | WP_001776113 | hypothetical protein | - |
| HTG85_RS00300 | 41961..42269 | + | 309 | WP_000212005 | hypothetical protein | - |
| HTG85_RS00305 | 42266..44467 | + | 2202 | WP_001776115 | type IA DNA topoisomerase | - |
| HTG85_RS00310 | 44464..44856 | + | 393 | WP_001776116 | H-NS family nucleoid-associated regulatory protein | - |
| HTG85_RS00315 | 45565..47472 | + | 1908 | WP_001776117 | type IV secretion system DNA-binding domain-containing protein | virb4 |
| HTG85_RS00320 | 47487..48233 | + | 747 | WP_001776118 | MobC family replication-relaxation protein | - |
| HTG85_RS00325 | 48226..48753 | + | 528 | WP_001776119 | phospholipase D family protein | - |
| HTG85_RS00330 | 48786..49217 | - | 432 | WP_001776120 | hypothetical protein | - |
| HTG85_RS00335 | 49236..49466 | + | 231 | WP_172687901 | hypothetical protein | - |
| HTG85_RS00340 | 49697..50662 | - | 966 | WP_001776122 | zincin-like metallopeptidase domain-containing protein | - |
Host bacterium
| ID | 3510 | GenBank | NZ_KU295131 |
| Plasmid name | pBK28009 | Incompatibility group | IncN |
| Plasmid size | 107760 bp | Coordinate of oriT [Strand] | 94592..94692 [+] |
| Host baterium | Escherichia coli strain BK28009 |
Cargo genes
| Drug resistance gene | dfrA5, qacE, sul1, blaTEM-1B, blaKPC-3 |
| Virulence gene | - |
| Metal resistance gene | arsR, arsD |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |