Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103055
Name   oriT_p369 in_silico
Organism   Escherichia coli strain 369
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KT779550 (40411..40499 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_p369
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2286 GenBank   WP_024143648
Name   nikB_HTG60_RS00185_p369 insolico UniProt ID   A0A023JAU0
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_024143648.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A023JAU0


Auxiliary protein

ID   946 GenBank   WP_001283947
Name   WP_001283947_p369 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1990 GenBank   WP_001289276
Name   trbC_HTG60_RS00180_p369 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 5603..35033

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTG60_RS00640 979..1188 - 210 Protein_0 hypothetical protein -
HTG60_RS00010 (p369_00001) 1188..1532 + 345 Protein_1 WbuC family cupin fold metalloprotein -
HTG60_RS00015 (p369_00002) 1579..2454 - 876 WP_013188473 extended-spectrum class A beta-lactamase CTX-M-1 -
HTG60_RS00020 (p369_00003) 2742..4004 - 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
HTG60_RS00025 (p369_00004) 4298..5452 + 1155 WP_001139957 site-specific integrase -
HTG60_RS00030 (p369_00005) 5603..6427 + 825 WP_001238939 conjugal transfer protein TraE traE
HTG60_RS00035 (p369_00006) 6512..7714 + 1203 WP_000976351 conjugal transfer protein TraF -
HTG60_RS00040 (p369_00007) 7774..8358 + 585 WP_000977522 histidine phosphatase family protein -
HTG60_RS00045 (p369_00008) 8753..9211 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HTG60_RS00050 (p369_00009) 9208..10026 + 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
HTG60_RS00055 (p369_00010) 10023..11171 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HTG60_RS00060 (p369_00011) 11168..11458 + 291 WP_001299214 hypothetical protein traK
HTG60_RS00065 (p369_00012) 11473..12024 + 552 WP_000014583 phospholipase D family protein -
HTG60_RS00070 (p369_00013) 12113..15880 + 3768 WP_001141527 LPD7 domain-containing protein -
HTG60_RS00075 (p369_00014) 15898..16245 + 348 WP_001055900 conjugal transfer protein traL
HTG60_RS00080 (p369_00015) 16242..16934 + 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
HTG60_RS00085 (p369_00016) 16945..17928 + 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
HTG60_RS00090 (p369_00017) 17931..19220 + 1290 WP_001272003 conjugal transfer protein TraO traO
HTG60_RS00095 (p369_00018) 19220..19924 + 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HTG60_RS00100 (p369_00019) 19924..20451 + 528 WP_001055569 conjugal transfer protein TraQ traQ
HTG60_RS00105 (p369_00020) 20502..20906 + 405 WP_000086958 IncI1-type conjugal transfer protein TraR traR
HTG60_RS00110 (p369_00021) 20970..21158 + 189 WP_001277255 putative conjugal transfer protein TraS -
HTG60_RS00115 (p369_00022) 21142..21942 + 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
HTG60_RS00120 (p369_00023) 22032..25076 + 3045 WP_021265678 IncI1-type conjugal transfer protein TraU traU
HTG60_RS00125 (p369_00024) 25076..25690 + 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
HTG60_RS00130 (p369_00025) 25657..26859 + 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
HTG60_RS00135 (p369_00026) 26888..27472 + 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HTG60_RS00140 (p369_00027) 27569..29737 + 2169 WP_021537350 DotA/TraY family protein traY
HTG60_RS00145 (p369_00028) 29787..30461 + 675 WP_223866377 plasmid IncI1-type surface exclusion protein ExcA -
HTG60_RS00150 (p369_00029) 30533..30742 - 210 WP_000062603 HEAT repeat domain-containing protein -
HTG60_RS00155 31026..31310 + 285 WP_031942457 hypothetical protein -
HTG60_RS00655 31375..31470 - 96 WP_001303310 DinQ-like type I toxin DqlB -
HTG60_RS00160 (p369_00031) 31969..32220 + 252 WP_001291964 hypothetical protein -
HTG60_RS00165 (p369_00032) 32292..32444 - 153 WP_001331364 Hok/Gef family protein -
HTG60_RS00170 (p369_00033) 32736..33944 + 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HTG60_RS00175 (p369_00034) 33963..35033 + 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
HTG60_RS00180 (p369_00035) 35026..37317 + 2292 WP_001289276 F-type conjugative transfer protein TrbC -

Host bacterium

ID   3498 GenBank   NZ_KT779550
Plasmid name   p369 Incompatibility group   IncI1
Plasmid size   107899 bp Coordinate of oriT [Strand]   40411..40499 [+]
Host baterium   Escherichia coli strain 369

Cargo genes

Drug resistance gene   blaCTX-M-1, sul2, tet(A)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -