Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103044 |
| Name | oriT_FWSEC0112|unnamed2  |
| Organism | Escherichia coli strain FWSEC0112 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_RRGD01000120 (43756..43809 [-], 54 nt) |
| oriT length | 54 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 54 nt
>oriT_FWSEC0112|unnamed2
CACAAGCATTGTAACATGCCCGGAACGTGCTTGTGTACAAAGCTTATCGTGCCT
CACAAGCATTGTAACATGCCCGGAACGTGCTTGTGTACAAAGCTTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1977 | GenBank | WP_086260088 |
| Name | t4cp2_C9Z62_RS27045_FWSEC0112|unnamed2 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73419.95 Da Isoelectric Point: 9.0081
>WP_086260088.1 type IV secretory system conjugative DNA transfer family protein [Escherichia coli]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLELYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLELYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1687..25040
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C9Z62_RS26995 (C9Z62_27055) | 1..1035 | - | 1035 | WP_000750512 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| C9Z62_RS27000 (C9Z62_27060) | 1048..1683 | - | 636 | WP_000934979 | A24 family peptidase | - |
| C9Z62_RS27005 (C9Z62_27065) | 1687..2169 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| C9Z62_RS27010 (C9Z62_27070) | 2235..2792 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| C9Z62_RS27015 (C9Z62_27075) | 2837..3946 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| C9Z62_RS27020 (C9Z62_27080) | 3937..5475 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| C9Z62_RS27025 (C9Z62_27085) | 5500..5994 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| C9Z62_RS27030 (C9Z62_27090) | 5978..7288 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| C9Z62_RS27035 (C9Z62_27095) | 7339..8982 | - | 1644 | WP_001035589 | PilN family type IVB pilus formation outer membrane protein | - |
| C9Z62_RS27040 (C9Z62_27100) | 8975..9505 | - | 531 | WP_001220542 | sigma 54-interacting transcriptional regulator | virb4 |
| C9Z62_RS27045 (C9Z62_27105) | 9552..11510 | - | 1959 | WP_086260088 | type IV secretory system conjugative DNA transfer family protein | - |
| C9Z62_RS27050 (C9Z62_27110) | 11526..12581 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| C9Z62_RS27055 (C9Z62_27115) | 12600..13739 | - | 1140 | WP_000790641 | TrbI/VirB10 family protein | virB10 |
| C9Z62_RS27060 (C9Z62_27120) | 13729..14430 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| C9Z62_RS27065 (C9Z62_27125) | 14496..15230 | - | 735 | WP_000432283 | type IV secretion system protein | virB8 |
| C9Z62_RS27075 (C9Z62_27135) | 15396..17753 | - | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| C9Z62_RS27080 (C9Z62_27140) | 17759..18079 | - | 321 | WP_086260089 | VirB3 family type IV secretion system protein | virB3 |
| C9Z62_RS28345 | 18150..18440 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| C9Z62_RS27090 (C9Z62_27150) | 18440..19024 | - | 585 | WP_001177114 | lytic transglycosylase domain-containing protein | virB1 |
| C9Z62_RS27095 (C9Z62_27155) | 19045..19443 | - | 399 | WP_001153669 | hypothetical protein | - |
| C9Z62_RS27100 (C9Z62_27160) | 19562..19999 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| C9Z62_RS27105 (C9Z62_27165) | 20005..21240 | - | 1236 | WP_063112255 | TcpQ domain-containing protein | - |
| C9Z62_RS27110 (C9Z62_27170) | 21243..21542 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| C9Z62_RS27115 (C9Z62_27175) | 21609..22244 | - | 636 | WP_077135686 | hypothetical protein | - |
| C9Z62_RS27120 (C9Z62_27180) | 22325..23083 | + | 759 | WP_224482110 | DUF5710 domain-containing protein | - |
| C9Z62_RS27125 (C9Z62_27185) | 23161..23397 | - | 237 | WP_000750964 | EexN family lipoprotein | - |
| C9Z62_RS27130 (C9Z62_27190) | 23401..24048 | - | 648 | WP_086260090 | type IV secretion system protein | - |
| C9Z62_RS27135 (C9Z62_27195) | 24054..25040 | - | 987 | WP_001028544 | type IV secretion system protein | virB6 |
| C9Z62_RS27140 (C9Z62_27200) | 25044..25301 | - | 258 | WP_000739144 | hypothetical protein | - |
| C9Z62_RS27145 (C9Z62_27205) | 25298..25600 | - | 303 | WP_077135687 | hypothetical protein | - |
| C9Z62_RS27150 (C9Z62_27210) | 25581..25838 | - | 258 | WP_001542015 | hypothetical protein | - |
| C9Z62_RS27155 (C9Z62_27215) | 25872..26525 | - | 654 | WP_021580487 | hypothetical protein | - |
| C9Z62_RS27625 | 26536..26706 | - | 171 | WP_000550721 | hypothetical protein | - |
| C9Z62_RS27160 (C9Z62_27220) | 26710..27153 | - | 444 | WP_000498521 | NfeD family protein | - |
| C9Z62_RS27165 (C9Z62_27225) | 27215..28168 | - | 954 | WP_072113668 | SPFH domain-containing protein | - |
| C9Z62_RS27170 (C9Z62_27230) | 28214..28408 | - | 195 | WP_086260091 | DUF1187 family protein | - |
| C9Z62_RS27175 (C9Z62_27235) | 28401..28853 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 3487 | GenBank | NZ_RRGD01000120 |
| Plasmid name | FWSEC0112|unnamed2 | Incompatibility group | IncI2 |
| Plasmid size | 57781 bp | Coordinate of oriT [Strand] | 43756..43809 [-] |
| Host baterium | Escherichia coli strain FWSEC0112 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |