Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103041
Name   oriT_pH226B in_silico
Organism   Escherichia coli strain H226B
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KX129784 (135719..136003 [-], 285 nt)
oriT length   285 nt
IRs (inverted repeats)      184..189, 191..196  (AAAAGT..ACTTTT)
Location of nic site      109..110
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 285 nt

>oriT_pH226B
GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCACATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTACAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2273 GenBank   WP_124035526
Name   TraI_2_HTI46_RS00655_pH226B insolico UniProt ID   _
Length   1011 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1011 a.a.        Molecular weight: 112979.96 Da        Isoelectric Point: 4.5108

>WP_124035526.1 MobH family relaxase [Escherichia coli]
MNFRALFLSIQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKFIEYPTRPAGI
QTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLL
SHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPS
SQSLLDWARENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDG
NDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVY
LNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVLRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVN
DAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQ
VEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAAFCNGPFFQLTYRDGS
LDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLE
LPQPRLNAPVAEEASSVAEQTNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYSATD
PQSSDFAQLPRETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA
RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDGEVTELEYVEI
ATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSGAVVNTKCI
AFNIPTLVAASDRAKVDIELIINKLKEVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-301)


  Protein structure



No available structure.




T4CP

ID   1974 GenBank   WP_000167418
Name   traD_HTI46_RS00660_pH226B insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77969.74 Da        Isoelectric Point: 8.7275

>WP_000167418.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLLVCLYRCKTAH
PYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKNNRPVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARH
YGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKPAGKVTLRQNVGLSYLNKYLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-245)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(410-638)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 198136..207304

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTI46_RS00995 193673..193873 - 201 WP_000357613 hypothetical protein -
HTI46_RS01000 193891..194967 - 1077 WP_001015069 hypothetical protein -
HTI46_RS01005 195502..196377 + 876 WP_001282731 RepB family plasmid replication initiator protein -
HTI46_RS01010 196824..197249 - 426 WP_154166887 hypothetical protein -
HTI46_RS01015 197731..198084 + 354 WP_000424120 pili assembly chaperone -
HTI46_RS01020 198136..198453 + 318 WP_000203871 type IV conjugative transfer system protein TraL traL
HTI46_RS01025 198465..199250 + 786 WP_000771920 TraE/TraK family type IV conjugative transfer system protein traE
HTI46_RS01030 199253..200485 + 1233 WP_000202110 type-F conjugative transfer system secretin TraK traK
HTI46_RS01035 200487..200927 + 441 WP_001224094 plasmid transfer protein HtdO -
HTI46_RS01040 200917..202275 + 1359 WP_000351840 IncHI-type conjugal transfer protein TrhB traB
HTI46_RS01045 202284..202796 + 513 WP_000429933 hypothetical protein -
HTI46_RS01050 202902..203654 + 753 WP_010892304 protein-disulfide isomerase HtdT -
HTI46_RS01055 203664..204614 + 951 WP_001022266 TraV family lipoprotein traV
HTI46_RS01060 204623..207304 + 2682 WP_001255537 IncHI-type conjugal transfer ATPase TrhC virb4
HTI46_RS01065 207913..208140 - 228 WP_172688232 hypothetical protein -
HTI46_RS01070 208949..209254 - 306 WP_172688233 hypothetical protein -

Host bacterium

ID   3484 GenBank   NZ_KX129784
Plasmid name   pH226B Incompatibility group   IncFIA
Plasmid size   209401 bp Coordinate of oriT [Strand]   135719..136003 [-]
Host baterium   Escherichia coli strain H226B

Cargo genes

Drug resistance gene   mcr-1.1
Virulence gene   -
Metal resistance gene   silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -