Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103032
Name   oriT_FWSEC0112|unnamed1 in_silico
Organism   Escherichia coli strain FWSEC0112
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRGD01000118 (36191..36279 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_FWSEC0112|unnamed1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2266 GenBank   WP_001303304
Name   nikB_C9Z62_RS26615_FWSEC0112|unnamed1 insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103960.41 Da        Isoelectric Point: 7.3474

>WP_001303304.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRNRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure



No available structure.




Auxiliary protein

ID   928 GenBank   WP_001283947
Name   WP_001283947_FWSEC0112|unnamed1 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1961 GenBank   WP_001289276
Name   trbC_C9Z62_RS26610_FWSEC0112|unnamed1 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1435..30813

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9Z62_RS26460 (C9Z62_26520) 130..1284 + 1155 WP_001139957 site-specific integrase -
C9Z62_RS26465 (C9Z62_26525) 1435..2259 + 825 WP_001238939 conjugal transfer protein TraE traE
C9Z62_RS26470 (C9Z62_26530) 2344..3546 + 1203 WP_000976351 conjugal transfer protein TraF -
C9Z62_RS26475 (C9Z62_26535) 3606..4190 + 585 WP_000977522 histidine phosphatase family protein -
C9Z62_RS26480 (C9Z62_26540) 4585..5043 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
C9Z62_RS26485 (C9Z62_26545) 5040..5858 + 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
C9Z62_RS26490 (C9Z62_26550) 5855..7003 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
C9Z62_RS26495 (C9Z62_26555) 7000..7290 + 291 WP_001299214 hypothetical protein traK
C9Z62_RS26500 (C9Z62_26560) 7305..7856 + 552 WP_000014583 phospholipase D family protein -
C9Z62_RS26505 (C9Z62_26565) 7946..11713 + 3768 WP_044707317 LPD7 domain-containing protein -
C9Z62_RS26510 (C9Z62_26570) 11731..12077 + 347 Protein_10 conjugal transfer protein -
C9Z62_RS26515 (C9Z62_26575) 12074..12766 + 693 WP_000138548 DotI/IcmL family type IV secretion protein traM
C9Z62_RS26520 (C9Z62_26580) 12777..13760 + 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
C9Z62_RS26525 (C9Z62_26585) 13763..15052 + 1290 WP_001272005 conjugal transfer protein TraO traO
C9Z62_RS26530 (C9Z62_26590) 15052..15756 + 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
C9Z62_RS26535 (C9Z62_26595) 15756..16283 + 528 WP_001055569 conjugal transfer protein TraQ traQ
C9Z62_RS26540 (C9Z62_26600) 16334..16738 + 405 WP_000086957 IncI1-type conjugal transfer protein TraR traR
C9Z62_RS26545 (C9Z62_26605) 16802..16990 + 189 WP_001277255 putative conjugal transfer protein TraS -
C9Z62_RS26550 (C9Z62_26610) 16974..17774 + 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
C9Z62_RS26555 (C9Z62_26615) 17864..20908 + 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
C9Z62_RS26560 (C9Z62_26620) 20908..21522 + 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
C9Z62_RS26565 (C9Z62_26625) 21489..22691 + 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
C9Z62_RS26570 (C9Z62_26630) 22720..23304 + 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
C9Z62_RS26575 (C9Z62_26635) 23401..25563 + 2163 WP_000698351 DotA/TraY family protein traY
C9Z62_RS26580 (C9Z62_26640) 25628..26290 + 663 WP_000653333 plasmid IncI1-type surface exclusion protein ExcA -
C9Z62_RS26585 (C9Z62_26645) 26362..26571 - 210 WP_000062602 HEAT repeat domain-containing protein -
C9Z62_RS27615 26914..27090 + 177 WP_001054898 hypothetical protein -
C9Z62_RS28470 27155..27250 - 96 WP_001303310 DinQ-like type I toxin DqlB -
C9Z62_RS26590 (C9Z62_26650) 27749..28000 + 252 WP_001291967 hypothetical protein -
C9Z62_RS26595 (C9Z62_26655) 28072..28224 - 153 WP_001303307 Hok/Gef family protein -
C9Z62_RS26600 (C9Z62_26660) 28516..29724 + 1209 WP_001303305 IncI1-type conjugal transfer protein TrbA trbA
C9Z62_RS26605 (C9Z62_26665) 29743..30813 + 1071 WP_086260038 IncI1-type conjugal transfer protein TrbB trbB
C9Z62_RS26610 (C9Z62_26670) 30806..33097 + 2292 WP_001289276 F-type conjugative transfer protein TrbC -

Host bacterium

ID   3475 GenBank   NZ_RRGD01000118
Plasmid name   FWSEC0112|unnamed1 Incompatibility group   IncI1
Plasmid size   75824 bp Coordinate of oriT [Strand]   36191..36279 [+]
Host baterium   Escherichia coli strain FWSEC0112

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -