Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103029 |
| Name | oriT_pABC149-MCR-1  |
| Organism | Escherichia coli strain ABC149 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KX013538 (13631..13683 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pABC149-MCR-1
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1958 | GenBank | WP_015059539 |
| Name | t4cp2_HTI75_RS00235_pABC149-MCR-1 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 33039..56496
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTI75_RS00170 | 28440..29093 | - | 654 | WP_170855322 | hypothetical protein | - |
| HTI75_RS00175 | 29105..30229 | - | 1125 | WP_000486716 | site-specific integrase | - |
| HTI75_RS00180 | 30552..30707 | - | 156 | WP_001358489 | hypothetical protein | - |
| HTI75_RS00415 | 30783..31014 | + | 232 | Protein_39 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTI75_RS00185 | 31011..32387 | - | 1377 | WP_000750519 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTI75_RS00190 | 32400..33035 | - | 636 | WP_000934977 | A24 family peptidase | - |
| HTI75_RS00195 | 33039..33521 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTI75_RS00200 | 33587..34144 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| HTI75_RS00205 | 34189..35298 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTI75_RS00210 | 35289..36827 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTI75_RS00215 | 36852..37346 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTI75_RS00220 | 37330..38652 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTI75_RS00225 | 38691..40334 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| HTI75_RS00230 | 40327..40863 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HTI75_RS00235 | 40910..42868 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HTI75_RS00240 | 42884..43939 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTI75_RS00245 | 43958..45097 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| HTI75_RS00250 | 45087..45788 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTI75_RS00255 | 45854..46588 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTI75_RS00260 | 46754..49111 | - | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| HTI75_RS00265 | 49117..49437 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTI75_RS00400 | 49508..49798 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HTI75_RS00275 | 49798..50382 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| HTI75_RS00280 | 50403..50801 | - | 399 | WP_001153665 | hypothetical protein | - |
| HTI75_RS00285 | 50920..51357 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HTI75_RS00290 | 51363..52598 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| HTI75_RS00295 | 52601..52900 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTI75_RS00300 | 52968..53249 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTI75_RS00305 | 53239..53490 | - | 252 | WP_000121741 | hypothetical protein | - |
| HTI75_RS00310 | 53590..54225 | - | 636 | WP_015059536 | hypothetical protein | - |
| HTI75_RS00315 | 54298..54585 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| HTI75_RS00320 | 54598..54852 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| HTI75_RS00325 | 54854..55495 | - | 642 | WP_001425343 | type IV secretion system protein | - |
| HTI75_RS00330 | 55501..56496 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| HTI75_RS00335 | 56500..56757 | - | 258 | WP_000739144 | hypothetical protein | - |
| HTI75_RS00340 | 56754..57107 | - | 354 | WP_223286767 | hypothetical protein | - |
| HTI75_RS00345 | 57327..57773 | - | 447 | WP_001243165 | hypothetical protein | - |
| HTI75_RS00350 | 57784..57954 | - | 171 | WP_000550720 | hypothetical protein | - |
| HTI75_RS00355 | 57958..58401 | - | 444 | WP_000964330 | NfeD family protein | - |
| HTI75_RS00360 | 58775..59728 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| HTI75_RS00365 | 59755..59931 | - | 177 | WP_000753050 | hypothetical protein | - |
| HTI75_RS00370 | 59924..60139 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| HTI75_RS00375 | 60132..60638 | - | 507 | WP_001326595 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 3472 | GenBank | NZ_KX013538 |
| Plasmid name | pABC149-MCR-1 | Incompatibility group | IncI2 |
| Plasmid size | 61228 bp | Coordinate of oriT [Strand] | 13631..13683 [-] |
| Host baterium | Escherichia coli strain ABC149 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |