Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103025 |
| Name | oriT_pBA76-MCR-1  |
| Organism | Escherichia coli strain BA76 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KX013540 (17113..17165 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pBA76-MCR-1
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1956 | GenBank | WP_015059539 |
| Name | t4cp2_HTI67_RS00265_pBA76-MCR-1 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 36293..59771
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTI67_RS00195 | 31515..32639 | - | 1125 | WP_000486716 | site-specific integrase | - |
| HTI67_RS00200 | 32962..33117 | - | 156 | WP_001358489 | hypothetical protein | - |
| HTI67_RS00425 | 33126..33497 | + | 372 | WP_236919413 | pilus assembly protein | - |
| HTI67_RS00435 | 34130..34348 | - | 219 | Protein_44 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTI67_RS00215 | 34367..35641 | - | 1275 | WP_235883108 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTI67_RS00220 | 35654..36289 | - | 636 | WP_000934977 | A24 family peptidase | - |
| HTI67_RS00225 | 36293..36775 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTI67_RS00230 | 36841..37404 | - | 564 | WP_034169414 | type 4 pilus major pilin | - |
| HTI67_RS00235 | 37454..38563 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTI67_RS00240 | 38554..40092 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTI67_RS00245 | 40117..40611 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTI67_RS00250 | 40595..41917 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTI67_RS00255 | 41956..43599 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| HTI67_RS00260 | 43592..44128 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HTI67_RS00265 | 44175..46133 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HTI67_RS00270 | 46149..47204 | - | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTI67_RS00275 | 47223..48362 | - | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| HTI67_RS00280 | 48352..49053 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTI67_RS00285 | 49119..49853 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTI67_RS00290 | 50019..52376 | - | 2358 | WP_172688158 | VirB4 family type IV secretion system protein | virb4 |
| HTI67_RS00295 | 52382..52702 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTI67_RS00430 | 52773..53063 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HTI67_RS00305 | 53063..53647 | - | 585 | WP_001177114 | lytic transglycosylase domain-containing protein | virB1 |
| HTI67_RS00310 | 53668..54066 | - | 399 | WP_001153669 | hypothetical protein | - |
| HTI67_RS00315 | 54185..54622 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HTI67_RS00320 | 54628..55863 | - | 1236 | WP_039022941 | toxin co-regulated pilus biosynthesis Q family protein | - |
| HTI67_RS00325 | 55866..56165 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTI67_RS00330 | 56232..56867 | - | 636 | WP_022645136 | hypothetical protein | - |
| HTI67_RS00335 | 56915..57706 | + | 792 | WP_022645137 | DUF5710 domain-containing protein | - |
| HTI67_RS00340 | 57870..58124 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| HTI67_RS00345 | 58126..58767 | - | 642 | WP_103736540 | type IV secretion system protein | - |
| HTI67_RS00350 | 58773..59771 | - | 999 | WP_103736541 | type IV secretion system protein | virB6 |
| HTI67_RS00355 | 59775..60032 | - | 258 | WP_000739144 | hypothetical protein | - |
| HTI67_RS00360 | 60029..60331 | - | 303 | WP_001360345 | hypothetical protein | - |
| HTI67_RS00365 | 60347..60613 | - | 267 | WP_228299791 | hypothetical protein | - |
| HTI67_RS00370 | 60834..61487 | - | 654 | WP_053899054 | hypothetical protein | - |
| HTI67_RS00375 | 61498..61668 | - | 171 | WP_000550721 | hypothetical protein | - |
| HTI67_RS00380 | 61672..62115 | - | 444 | WP_103736542 | NfeD family protein | - |
| HTI67_RS00385 | 62489..63442 | - | 954 | WP_072109880 | SPFH domain-containing protein | - |
| HTI67_RS00390 | 63469..63645 | - | 177 | WP_000753050 | hypothetical protein | - |
| HTI67_RS00395 | 63638..63853 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| HTI67_RS00400 | 63846..64352 | - | 507 | WP_039022938 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 3468 | GenBank | NZ_KX013540 |
| Plasmid name | pBA76-MCR-1 | Incompatibility group | IncI2 |
| Plasmid size | 64942 bp | Coordinate of oriT [Strand] | 17113..17165 [-] |
| Host baterium | Escherichia coli strain BA76 |
Cargo genes
| Drug resistance gene | blaCTX-M-64, mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |