Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103021
Name   oriT_LM6771 in_silico
Organism   Escherichia coli strain 06K2206
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KX009507 (73330..73435 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_LM6771
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2260 GenBank   WP_050868819
Name   Relaxase_HTH91_RS00460_LM6771 insolico UniProt ID   A0A142BMF1
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75234.98 Da        Isoelectric Point: 10.0285

>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A142BMF1


T4CP

ID   1953 GenBank   WP_011091092
Name   t4cp2_HTH91_RS00170_LM6771 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79491.81 Da        Isoelectric Point: 4.8270

>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 76231..87722

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTH91_RS00440 71315..71626 + 312 WP_011091065 hypothetical protein -
HTH91_RS00445 71759..72295 + 537 WP_011091066 hypothetical protein -
HTH91_RS00450 72796..73161 - 366 WP_011091067 hypothetical protein -
HTH91_RS00545 73176..73754 + 579 WP_223811519 plasmid mobilization protein MobA -
HTH91_RS00460 73741..75720 + 1980 WP_050868819 TraI/MobA(P) family conjugative relaxase -
HTH91_RS00465 75734..76234 + 501 WP_172688371 DotD/TraH family lipoprotein -
HTH91_RS00470 76231..77010 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
HTH91_RS00475 77021..78184 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
HTH91_RS00480 78174..78434 + 261 WP_004187310 IcmT/TraK family protein traK
HTH91_RS00550 78459..81671 + 3213 WP_011091070 LPD7 domain-containing protein -
HTH91_RS00490 81595..82149 + 555 WP_011154467 hypothetical protein traL
HTH91_RS00495 82150..82347 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
HTH91_RS00500 82334..82744 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
HTH91_RS00505 82743..83525 + 783 WP_011091073 DotI/IcmL family type IV secretion protein traM
HTH91_RS00510 83534..84685 + 1152 WP_011091074 DotH/IcmK family type IV secretion protein traN
HTH91_RS00515 84697..86046 + 1350 WP_011091075 conjugal transfer protein TraO traO
HTH91_RS00520 86058..86762 + 705 WP_011091076 conjugal transfer protein TraP traP
HTH91_RS00525 86786..87316 + 531 WP_011091077 conjugal transfer protein TraQ traQ
HTH91_RS00530 87333..87722 + 390 WP_011091078 DUF6750 family protein traR
HTH91_RS00535 87768..88262 + 495 WP_011091080 hypothetical protein -

Host bacterium

ID   3464 GenBank   NZ_KX009507
Plasmid name   LM6771 Incompatibility group   IncL/M
Plasmid size   89090 bp Coordinate of oriT [Strand]   73330..73435 [+]
Host baterium   Escherichia coli strain 06K2206

Cargo genes

Drug resistance gene   blaTEM-1B, aac(6')-Ib-cr, blaOXA-1, catB3, ARR-3, qacE, sul1, armA, msr(E), mph(E), blaCTX-M-3
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -